bms193885  (Tocris)

Journal: Molecular Brain

Article Title: Identification of VGLUT3-expressing LTMRs-recruited spinal circuits for itch inhibition

doi: 10.1186/s13041-025-01245-3

Figure Lengend Snippet: VGLUT3 + sensory fibers alleviate mechanical and chemical itch via spinal NPY-Y1R and DYN-KOR-signaling pathway, respectively. A, F Schematic of experimental design. B, C Pretreatment of Y1 receptor antagonist (BMS193885) does not alter the inhibitory effect of VGLUT3 + fibers on both histamine and chloroquine itch models. D, E Pretreatment of BMS193885 abrogates the inhibitory effect of VGLUT3 + fibers on mechanical itch and reduces the degree of inhibition. G, H Pretreatment of KOR antagonist (norBNI) attenuates the degree of itch inhibition mediated by VGLUT3 + fibers on both histamine and chloroquine itch models. I,J Pretreatment of KOR antagonist (norBNI) does not alter VGLUT3 + fiber-mediated suppression of mechanical itch. Data are represented as mean with SEM and assessed using two-tailed unpaired t test, exact p-values are reported for all statistical comparisons

Article Snippet: The nor-Binaltorphimine (nor-BNI) (Cat# AB120078 , Abcam) and BMS193885 (Cat#HY-120619, MCE) were dissolved in saline.

Techniques: Inhibition, Two Tailed Test

Journal: Molecular Brain

Article Title: Identification of VGLUT3-expressing LTMRs-recruited spinal circuits for itch inhibition

doi: 10.1186/s13041-025-01245-3

Figure Lengend Snippet: Schematic model of the spinal inhibitory circuit for DRG VGLUT3 -mediated suppression of itch. A Optogenetic activation of VGLUT3-expressing LTMR terminals inhibits itch signaling in the spinal cord through distinct pathways. Chemical itch is suppressed via a dynorphin (DYN)-mediated inhibitory pathway, where VGLUT3⁺ LTMR activation recruits DYN⁺ inhibitory interneurons to suppress κ-opioid receptor (KOR)-expressing pruriceptive neurons. This anti-pruritic effect is abolished by the selective KOR antagonist norBNI. B Mechanical itch is inhibited through a distinct neuropeptide Y (NPY)-dependent pathway, where VGLUT3⁺ LTMR activation recruits NPY⁺ inhibitory interneurons to suppress itch-transmission neurons via Y1 receptor (Y1R) signaling. This anti-pruritic effect is selectively blocked by the Y1R antagonist BMS193885

Article Snippet: The nor-Binaltorphimine (nor-BNI) (Cat# AB120078 , Abcam) and BMS193885 (Cat#HY-120619, MCE) were dissolved in saline.

Techniques: Activation Assay, Expressing, Transmission Assay