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paper n a thp1 lucia isg cells invivogen thpl isg thp1 lucia nf κb cells invivogen thpl nfkbv2 raw lucia isg cells invivogen rawl isg bmdms  (InvivoGen)


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    InvivoGen paper n a thp1 lucia isg cells invivogen thpl isg thp1 lucia nf κb cells invivogen thpl nfkbv2 raw lucia isg cells invivogen rawl isg bmdms
    Paper N A Thp1 Lucia Isg Cells Invivogen Thpl Isg Thp1 Lucia Nf κb Cells Invivogen Thpl Nfkbv2 Raw Lucia Isg Cells Invivogen Rawl Isg Bmdms, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdms/THP1-Lucia+NF-%CE%BAB+Cells/pm42313565-218-9-14
    Average 95 stars, based on 78 article reviews
    paper n a thp1 lucia isg cells invivogen thpl isg thp1 lucia nf κb cells invivogen thpl nfkbv2 raw lucia isg cells invivogen rawl isg bmdms - by Bioz Stars, 2026-11
    95/100 stars

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    Article Title: Pharmacological targeting of the NLRP3 LRR domain with isothiazolinones overcomes CRID3-resistant inflammation.
    Article Snippet: For activation of the canonical NLRP3 inflammasome, cells were primed for 4 h with 100 ng/mL LPS (InvivoGen, tlrlsmlps) and were stimulated with 5 mM ATP (Roche, 10127531001) or 20 μM nigericin (Cayman, 11437) for indicated time with or without 5 μM LOC14 (Selleckchem, S0321), 1 μM CRID3 (Selleckchem, S7809), 5 μM 1,2-Benzisothiazol-3(2H)-one (Tokyo Chemical Industry, B3767), 5 μM 1-(cyclopropylcarbonyl)piperazine (Tokyo Chemical Industry, C3112), 5 μM Isothiazol-3(2H)one (Tokyo Chemical Industry, I1172), 5 μM Isothiazol (Tokyo Chemical Industry, I0982), 5 μM Thiazole (Tokyo Chemical Industry, T0185), 5 μM Imidazole (Tokyo Chemical Industry, I001), 1, 5, and 10 μM of 4,5-dichloro-2-n-octyl-4-isothiazoline-3one (MedChemExpress, HY-W041308), and 1, 5, and 10 μM of methylisothiazolinone (TargetMol, T19774).

    Article Title: K27-linked ubiquitination restrains the NOD2-RIP2 signaling pathway
    Article Snippet: HEK293 cells (ATCC) were maintained in DMEM (1×) +Glutamax-I (Gibco, Life technology) supplemented with 10% FCS (Gibco, Life technology) and 100 U/ml penicillin/streptomycin (Sigma-Aldrich).

    Article Title: Independent mechanisms of inflammation and myeloid bias in VEXAS syndrome
    Article Snippet: The cell pellets were then resuspended in 400 μl Triton lysis buffer (20 mM Tris HCl pH 7.4, 135 mM NaCl, 1.5 mM MgCl 2 , 1 mM EGTA, 1% (v/v) Triton X-100, 10% (v/v) glycerol) containing complete protease and phosphatase inhibitor cocktail and 10 mM N -ethylmaleimide and lysed for 15 min on ice followed by centrifugation at 20,000 g for 10 min. 7.5% of input was set aside, and 25 μl of prewashed magnetic linear-polyubiquitin-specific (Lifesensors, UM406M) or Lys63-polyubiquitin-specific (Lifesensors, UM404M) TUBE beads were added to the remaining lysate.

    Article Title: Extinguishing the flames of inflammation: retardant effect of chlorquinaldol on NLRP3-driven diseases.
    Article Snippet: The luminescence were recorded using Microplate Reader (Tecan, Infinite 200Pro).

    Concentration Assay:

    Article Title: Methylglyoxal is an antibacterial effector produced by macrophages during infection.
    Article Snippet: .. BMDMs were stimulated for 18-24 hours with PAM3CSK4 (Invivogen) at a final concentration of 100ng/mL or recombinant mouse IFN-γ (R&D Systems, 485-MI) and used at 6.25 ng/mL. ..

    Article Title: Methylglyoxal is an antibacterial effector produced by macrophages during infection.
    Article Snippet: .. Briefly, BMDMs were stimulated with PAM3CSK4 (Invivogen) at a final concentration of 100ng/mL, recombinant mouse IFN-γ (R&D Systems, 485-MI) and used at 6.25 ng/mL or TBHP positive control for 4 hours. ..

    Recombinant:

    Article Title: Methylglyoxal is an antibacterial effector produced by macrophages during infection.
    Article Snippet: .. BMDMs were stimulated for 18-24 hours with PAM3CSK4 (Invivogen) at a final concentration of 100ng/mL or recombinant mouse IFN-γ (R&D Systems, 485-MI) and used at 6.25 ng/mL. ..

    Article Title: Methylglyoxal is an antibacterial effector produced by macrophages during infection.
    Article Snippet: .. Briefly, BMDMs were stimulated with PAM3CSK4 (Invivogen) at a final concentration of 100ng/mL, recombinant mouse IFN-γ (R&D Systems, 485-MI) and used at 6.25 ng/mL or TBHP positive control for 4 hours. ..

    Positive Control:

    Article Title: Methylglyoxal is an antibacterial effector produced by macrophages during infection.
    Article Snippet: .. Briefly, BMDMs were stimulated with PAM3CSK4 (Invivogen) at a final concentration of 100ng/mL, recombinant mouse IFN-γ (R&D Systems, 485-MI) and used at 6.25 ng/mL or TBHP positive control for 4 hours. ..



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    Co-culture of BMDMs and MEFs in stiff 3D hydrogels increases CD54 and CD140a expression on macrophages. BMDMs and MEFs were co-cultured in 3D collagen-vitronectin, collagen-vitronectin-5% alginate scaffolds, or 2D controls for 24 hours. The viability, proportion of each cell type and surface expression of key markers was determined by flow cytometry. (A) Representative gating strategy for the separation and analysis of co-cultured BMDMs and MEFs by flow cytometry. Sample shown are BMDMs and MEFs cultured in stiff collagen-vitronectin-5% alginate hydrogels. (B) Overall cell viability. Note that this represents a mixture of BMDMs and MEFs. (C) Proportion of BMDMs (CD45 + CD11b + F4/80 + ) and MEFs (CD45 - CD11b - F4/80 - ) in the indicated co-culture conditions. (D, E) Geometric mean fluorescence intensity (gMFI) of CD11b and F4/80 expression on co-cultured BMDMs. (F) Representative histograms for CD54 and CD140a expression on BMDMs (left) and MEFs (right). (G–J) The relative expression of CD54 (G, H) and CD140a (I, J) on BMDMs and MEFs after co-culture (G, I) or single-culture (H–J) in the indicated models. Some single-cultured BMDMs were also activated with LPS and IFN-γ or IL-4 and IL-13, as indicated. Data in G-J are the relative gMFI, calculated by dividing gMFI values by the mean gMFI of unactivated BMDMs cultured in 2D. B-E and G-J show the pooled means of 3–4 independent experiments. Errors are SEM. Groups in B, D, E, H and J were tested by one-way ANOVA; C, G and I by two-way ANOVA where for the latter overall ANOVA results are indicated above each graph. Post-hoc analyses were performed for one- and two-way ANOVAs where significance between groups is indicated as **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant.

    Journal: Frontiers in Immunology

    Article Title: Co-culture with fibroblasts in stiff 3D scaffolds increases CD54 and CD140a expression on macrophages

    doi: 10.3389/fimmu.2026.1771248

    Figure Lengend Snippet: Co-culture of BMDMs and MEFs in stiff 3D hydrogels increases CD54 and CD140a expression on macrophages. BMDMs and MEFs were co-cultured in 3D collagen-vitronectin, collagen-vitronectin-5% alginate scaffolds, or 2D controls for 24 hours. The viability, proportion of each cell type and surface expression of key markers was determined by flow cytometry. (A) Representative gating strategy for the separation and analysis of co-cultured BMDMs and MEFs by flow cytometry. Sample shown are BMDMs and MEFs cultured in stiff collagen-vitronectin-5% alginate hydrogels. (B) Overall cell viability. Note that this represents a mixture of BMDMs and MEFs. (C) Proportion of BMDMs (CD45 + CD11b + F4/80 + ) and MEFs (CD45 - CD11b - F4/80 - ) in the indicated co-culture conditions. (D, E) Geometric mean fluorescence intensity (gMFI) of CD11b and F4/80 expression on co-cultured BMDMs. (F) Representative histograms for CD54 and CD140a expression on BMDMs (left) and MEFs (right). (G–J) The relative expression of CD54 (G, H) and CD140a (I, J) on BMDMs and MEFs after co-culture (G, I) or single-culture (H–J) in the indicated models. Some single-cultured BMDMs were also activated with LPS and IFN-γ or IL-4 and IL-13, as indicated. Data in G-J are the relative gMFI, calculated by dividing gMFI values by the mean gMFI of unactivated BMDMs cultured in 2D. B-E and G-J show the pooled means of 3–4 independent experiments. Errors are SEM. Groups in B, D, E, H and J were tested by one-way ANOVA; C, G and I by two-way ANOVA where for the latter overall ANOVA results are indicated above each graph. Post-hoc analyses were performed for one- and two-way ANOVAs where significance between groups is indicated as **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant.

    Article Snippet: Where indicated, BMDM monocultures were activated with 10 ng/mL lipopolysaccharide (LPS; tlrl-3pelps, Invivogen) and 10 ng/mL murine interferon gamma (IFNγ; Abcam), or 10 ng/mL murine interleukin (IL-4; PeproTech) and 10 ng/mL IL-13 (PeproTech), as indicated.

    Techniques: Co-culture Assay, Expressing, Cell Culture, Flow Cytometry, Analysis, Fluorescence