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Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and <t>BIRC3</t> ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Birc3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/birc3/product/Proteintech
Average 93 stars, based on 27 article reviews

birc3 - by Bioz Stars, 2026-03

93/100 stars

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1) Product Images from "Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35"

Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

Journal: BMC Cancer

doi: 10.1186/s12885-025-14925-z

Figure Legend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Techniques Used: Transfection

Image Search Results

Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC3 and XAF1 genes depending on PD-1 expression, The U Mann Whitney Test.

Article Snippet: The following TaqMan probes were used for the reaction: NAIP (Hs03037952_m1), BIRC2 (Hs00357350_m1), BIRC3 (Hs00154109_m1), XIAP (Hs00236913_m1), BIRC5 (Hs00153353_m1), BIRC6 (Hs00212288_m1), BIRC8 (Hs01057786), XAF1 (Hs01550138_m1), DIABLO (Hs00219876_m1), CASP3 (Hs00234387_m1) and CASP9 (Hs00962278_m1).

Techniques: Expressing, MANN-WHITNEY

Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 (p = 0.025) , BIRC3 and BIRC5 genes depending on survival rate (< 20 months and > 20 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on survival rate (< 12 months and > 12 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and survival rate ( r = -0.478 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and survival rate ( r = -0.536 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression free survival (< 20 months and > 20 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC2 gene expression (logRQ) and progression-free survival ( r =-0.481 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Mean (logRQ ± SE) expression of BIRC2 and BIRC3 genes depending on progression-free survival (< 12 months and > 12 months), (The U Mann Whitney Test)

Techniques: Expressing, MANN-WHITNEY

Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Scatterplot between BIRC3 gene expression (logRQ) and progression-free survival ( r =-0.536 p < 0.05 Spearman’s rank correlations).

Techniques: Gene Expression

Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Forest plot of odds ratios (OR) from univariate logistic regression for dichotomized survival endpoints (OS/PFS) in the intersection set, using BIRC2 expression (logRQ) as predictor.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Techniques: Expressing

Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Kaplan–Meier overall survival stratified by BIRC2 expression group (high vs. low). Log-rank p = 0.023* BIRC2 group (high = logRQ BIRC2 >-0.077794 (median) /low = logRQ BIRC2 <=-0.077794).

Techniques: Expressing

Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

Journal: Scientific Reports

Article Title: Reduced expression of BIRC2 and BIRC3 associated with longer survival in pediatric high-grade gliomas

doi: 10.1038/s41598-026-35887-7

Figure Lengend Snippet: Kaplan–Meier overall survival stratified by BIRC3 expression group (high vs. low). Log-rank p = 0.1824. BIRC3 group (high = logRQ BIRC3 >-0.316210 (median) /low = logRQ BIRC3 <=-0.316210).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Chidamide impedes glycolysis but increases ferroptosis and cisplatin sensitivity of lung cancer cells through downregulating USP35

doi: 10.1186/s12885-025-14925-z

Figure Lengend Snippet: Chidamide regulated the proteins associated with glycolysis, ferroptosis and DDP sensitivity by down-regulating USP35. A549 cells with treatment of 0.8 µM chidamide and H460 cells with treatment of 3.2 µM chidamide or not were transfected with oe-NC or oe-USP35. A - D WB analysis ( A ) was administrated for protein examination of PKM2 ( B ), FPN ( C ), and BIRC3 ( D ) in A549 cells. E - H WB results ( E ) for PKM2 ( F ), FPN ( G ), and BIRC3 ( H ) in H460 cells. */# P < 0.05, **/##/&& P < 0.01, ***/###/&&& P < 0.001. * : compared to oe-NC group, # : compared to oe-USP35 group, & : compared to chidamide + oe-NC group

Article Snippet: The primary antibodies against USP35 (proteintech, Wuhan, China, 24559-1-AP), PKM2 (proteintech, 15822-1-AP), FPN (proteintech, 26601-1-AP), BIRC3 (proteintech, 24304-1-AP), β-actin (proteintech, 20536-1-AP) were incubated overnight at 4°C.

Techniques: Transfection

BIRC3 was the key regulator of 6-TG against EV71 infection. ( A ) HeLa cells were infected with EV71 at an MOI of 1 in the presence of NSC23766 at different concentrations (0, 5, 10, 30, 50, 100 µM). After 24 h, the intracellular EV71 VP1 (green) was examined via ICW assay and normalized by DRAQ5 (red). The percent of EV71 inhibition was analyzed by Odyssey software, and the intracellular EV71 VP1 of EV71-infected cells without NSC23766 treatment were considered as a negative control. ( B ) Identification of 14 common differential expressed genes (DEG) from untreated or 6-TG-treated HUVEC database (Control vs. 6-TG) and uninfected or EV71-infected mice database (GSE123550) (Mock vs. EV71) through SangerBox 3.0 online tools. Advanced Pie plot was performed using the OmicStudio tools at https://www.omicstudio.cn/tool . ( C ) The heatmap of the 14 common DEGs from Control vs. 6-TG group and Mock vs. EV71 group. Advanced Heatmap Plots was performed using the OmicStudio tools at https://www.omicstudio.cn . ( D and E ) HeLa cells were incubated with 1 or 3 µM 6-TG for 2 h. Then the BIRC3 mRNA was quantified by quantitative real-time PCR, normalized to GAPDH ( D ). The BIRC3 protein levels were quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( E ). ( F , G and H ) HeLa cells were infected with EV71 at an MOI of 0.5 for 24 h, and the EV71 mRNA and BIRC3 mRNA was quantified via quantitative real-time PCR, normalized against GAPDH ( F and G ). The BIRC3 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( H ). ( I , J and K ) HeLa cells were infected with EV71 at an MOI of 0.5 in the presence of 6-TG at 1 µM. The BIRC3 and EV71 VP1 expression was quantified via western blot analysis, normalized against β-actin. The intensity of BIRC3 was quantified by ImageJ software ( I ). The EV71 mRNA and BIRC3 mRNA was quantified via quantitative real-time PCR, normalized against GAPDH ( J and K ). Data were pooled from three ( D - K ) independent experiments, and were shown as the mean ± SEM (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet: BIRC3 was the key regulator of 6-TG against EV71 infection. ( A ) HeLa cells were infected with EV71 at an MOI of 1 in the presence of NSC23766 at different concentrations (0, 5, 10, 30, 50, 100 µM). After 24 h, the intracellular EV71 VP1 (green) was examined via ICW assay and normalized by DRAQ5 (red). The percent of EV71 inhibition was analyzed by Odyssey software, and the intracellular EV71 VP1 of EV71-infected cells without NSC23766 treatment were considered as a negative control. ( B ) Identification of 14 common differential expressed genes (DEG) from untreated or 6-TG-treated HUVEC database (Control vs. 6-TG) and uninfected or EV71-infected mice database (GSE123550) (Mock vs. EV71) through SangerBox 3.0 online tools. Advanced Pie plot was performed using the OmicStudio tools at https://www.omicstudio.cn/tool . ( C ) The heatmap of the 14 common DEGs from Control vs. 6-TG group and Mock vs. EV71 group. Advanced Heatmap Plots was performed using the OmicStudio tools at https://www.omicstudio.cn . ( D and E ) HeLa cells were incubated with 1 or 3 µM 6-TG for 2 h. Then the BIRC3 mRNA was quantified by quantitative real-time PCR, normalized to GAPDH ( D ). The BIRC3 protein levels were quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( E ). ( F , G and H ) HeLa cells were infected with EV71 at an MOI of 0.5 for 24 h, and the EV71 mRNA and BIRC3 mRNA was quantified via quantitative real-time PCR, normalized against GAPDH ( F and G ). The BIRC3 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( H ). ( I , J and K ) HeLa cells were infected with EV71 at an MOI of 0.5 in the presence of 6-TG at 1 µM. The BIRC3 and EV71 VP1 expression was quantified via western blot analysis, normalized against β-actin. The intensity of BIRC3 was quantified by ImageJ software ( I ). The EV71 mRNA and BIRC3 mRNA was quantified via quantitative real-time PCR, normalized against GAPDH ( J and K ). Data were pooled from three ( D - K ) independent experiments, and were shown as the mean ± SEM (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Rabbit anti-BIRC3 antibody Mouse anti-Flag tag antibody , ABclonal (Wuhan, China) Servicebio (Wuhan, China) , A0833 GB15938-100.

Techniques: Infection, Inhibition, Software, Negative Control, Control, Incubation, Real-time Polymerase Chain Reaction, Western Blot, Expressing

BIRC3 promoted EV71 replication and reversed the anti-EV71 effect of 6-TG. ( A and B ) HeLa cells were transfected with three specific siRNAs targeting BIRC3 (siBIRC3 #1, siBIRC3 #2, siBIRC3 #3) or control siRNA (siNC) for 30 h. The cells were harvested and the BIRC3 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( A ), the BIRC3 protein levels were evaluated by western blot assay, normalized to β-actin ( B ). ( C and D ) HeLa cells were transfected with siBIRC3 or siNC for 30 h, and then infected with EV71 at an MOI of 0.5 for 24 h. The cells were harvested and the EV71 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( C ). The EV71 VP1 protein expression was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( D ). ( E and F ) HeLa cells were transfected with 1 µg plasmid encoding BIRC3 (pBIRC3) or control plasmid (pVector). After 30 h, the cells were collected, and the BIRC3 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( E ), the flag-BIRC3 protein expression was evaluated by western blot assay, normalized to β-actin ( F ). ( G and H ) HeLa cells were transfected with 1 µg pBIRC3 or pVector for 30 h, and then infected with EV71 at an MOI of 0.5 for 24 h. The cells were harvested and EV71 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( G ). The EV71 VP1 protein expression in harvested cells was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( H ). ( I ) HeLa cells were transfected with 1 µg pBIRC3 or pVector for 30 h, and next infected with EV71 at an MOI of 0.5 for 2 h, then the cells were washed with PBS and incubated with clear culture medium. After 24 h, the culture supernatant was collected and added to Vero cells, the viral titers were calculated as the TCID 50 after 72 h incubation. ( J and K ) HeLa cells were transfected with 1 µg pBIRC3 or an empty Vector for 30 h, then the cells were infected with EV71 at an MOI of 0.5 in the presence of 1 µM 6-TG for 24 h. The EV71 RNA levels and the BIRC3 RNA levels in harvested cells were quantified via quantitative real-time PCR, normalized against GAPDH. ( L ) HeLa cells were transfected with 1 µg pBIRC3 or an empty Vector for 30 h, then the cells were infected with EV71 at an MOI of 0.5 for 2 h. After washing with PBS, the cells were incubated with 1 µM 6-TG for 24 h. The culture supernatant was collected and added to Vero cells, the viral titers were calculated as the TCID 50 after 72 h incubation. Data were pooled from three ( A , C-E and G-L ) or two ( B and F ) independent experiments, and were shown as the mean ± SEM (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet: BIRC3 promoted EV71 replication and reversed the anti-EV71 effect of 6-TG. ( A and B ) HeLa cells were transfected with three specific siRNAs targeting BIRC3 (siBIRC3 #1, siBIRC3 #2, siBIRC3 #3) or control siRNA (siNC) for 30 h. The cells were harvested and the BIRC3 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( A ), the BIRC3 protein levels were evaluated by western blot assay, normalized to β-actin ( B ). ( C and D ) HeLa cells were transfected with siBIRC3 or siNC for 30 h, and then infected with EV71 at an MOI of 0.5 for 24 h. The cells were harvested and the EV71 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( C ). The EV71 VP1 protein expression was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( D ). ( E and F ) HeLa cells were transfected with 1 µg plasmid encoding BIRC3 (pBIRC3) or control plasmid (pVector). After 30 h, the cells were collected, and the BIRC3 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( E ), the flag-BIRC3 protein expression was evaluated by western blot assay, normalized to β-actin ( F ). ( G and H ) HeLa cells were transfected with 1 µg pBIRC3 or pVector for 30 h, and then infected with EV71 at an MOI of 0.5 for 24 h. The cells were harvested and EV71 RNA levels were quantified via quantitative real-time PCR, normalized against GAPDH ( G ). The EV71 VP1 protein expression in harvested cells was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals was quantified by ImageJ software ( H ). ( I ) HeLa cells were transfected with 1 µg pBIRC3 or pVector for 30 h, and next infected with EV71 at an MOI of 0.5 for 2 h, then the cells were washed with PBS and incubated with clear culture medium. After 24 h, the culture supernatant was collected and added to Vero cells, the viral titers were calculated as the TCID 50 after 72 h incubation. ( J and K ) HeLa cells were transfected with 1 µg pBIRC3 or an empty Vector for 30 h, then the cells were infected with EV71 at an MOI of 0.5 in the presence of 1 µM 6-TG for 24 h. The EV71 RNA levels and the BIRC3 RNA levels in harvested cells were quantified via quantitative real-time PCR, normalized against GAPDH. ( L ) HeLa cells were transfected with 1 µg pBIRC3 or an empty Vector for 30 h, then the cells were infected with EV71 at an MOI of 0.5 for 2 h. After washing with PBS, the cells were incubated with 1 µM 6-TG for 24 h. The culture supernatant was collected and added to Vero cells, the viral titers were calculated as the TCID 50 after 72 h incubation. Data were pooled from three ( A , C-E and G-L ) or two ( B and F ) independent experiments, and were shown as the mean ± SEM (* P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Rabbit anti-BIRC3 antibody Mouse anti-Flag tag antibody , ABclonal (Wuhan, China) Servicebio (Wuhan, China) , A0833 GB15938-100.

Techniques: Transfection, Control, Real-time Polymerase Chain Reaction, Western Blot, Infection, Expressing, Software, Plasmid Preparation, Incubation

BIRC3 knockdown affected the cleavage function of EV71 2A pro and the expression of EV71 3AB. HeLa cells were transfected with plasmid GFP-2A pro , GFP-2B, GFP-3AB, GFP-3C, GFP-3D and HA-2C for 24 h, then were transfected with siBIRC3 or siNC for 30 h. The cells were harvested and the expression of eIF4G, GFP-tag, HA-tag was evaluated by western blot assay, normalized to β-actin or GAPDH. The intensity of western blot bands signals of cleaved eIF4G, GFP-2B/3AB/3 C/3D, HA-2 C, β-actin and GAPDH were quantified by ImageJ software. Data were pooled from three independent experiments, and were shown as the mean ± SEM (ns, no significant, * P < 0.05, ** P < 0.01)

Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet: BIRC3 knockdown affected the cleavage function of EV71 2A pro and the expression of EV71 3AB. HeLa cells were transfected with plasmid GFP-2A pro , GFP-2B, GFP-3AB, GFP-3C, GFP-3D and HA-2C for 24 h, then were transfected with siBIRC3 or siNC for 30 h. The cells were harvested and the expression of eIF4G, GFP-tag, HA-tag was evaluated by western blot assay, normalized to β-actin or GAPDH. The intensity of western blot bands signals of cleaved eIF4G, GFP-2B/3AB/3 C/3D, HA-2 C, β-actin and GAPDH were quantified by ImageJ software. Data were pooled from three independent experiments, and were shown as the mean ± SEM (ns, no significant, * P < 0.05, ** P < 0.01)

Article Snippet: Rabbit anti-BIRC3 antibody Mouse anti-Flag tag antibody , ABclonal (Wuhan, China) Servicebio (Wuhan, China) , A0833 GB15938-100.

Techniques: Knockdown, Expressing, Transfection, Plasmid Preparation, Western Blot, Software

6-TG suppressed EV71 replication by diminishing BIRC3-mediated autophagy. ( A ) HeLa cells were Mock-infected or EV71 (MOI = 0.5) infected for 12 h, then cells were fixed for immunostaining using EV71 2C antibody (red). Nuclei were labeled with DAPI (blue). Scale bars, 10 μm. The white arrows showed the formed GFP-LC3 puncta. ( B ) HeLa cells were infected with EV71 at an MOI of 0.5, and the cells were harvested at 12, 24 hpi (hours post infection). The EV71 VP1, LC3 I, LC3 II and P62 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( C ) HeLa cells were infected with EV71 at an MOI of 0.5 in the presence of 1 µM 6-TG. After 24 h, the EV71 VP1, LC3 I, LC3 II and P62 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( D ) HeLa cells were transfected with siBIRC3 or siNC for 30 h, then the cells were infected with EV71 at an MOI of 0.5 for 24 h. The BIRC3, LC3 I, LC3 II, P62 and EV71 VP1 expression in harvested cells was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. Data were pooled from three ( B-D ) or two ( A ) independent experiments, and were shown as the mean ± SEM (ns, no significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet: 6-TG suppressed EV71 replication by diminishing BIRC3-mediated autophagy. ( A ) HeLa cells were Mock-infected or EV71 (MOI = 0.5) infected for 12 h, then cells were fixed for immunostaining using EV71 2C antibody (red). Nuclei were labeled with DAPI (blue). Scale bars, 10 μm. The white arrows showed the formed GFP-LC3 puncta. ( B ) HeLa cells were infected with EV71 at an MOI of 0.5, and the cells were harvested at 12, 24 hpi (hours post infection). The EV71 VP1, LC3 I, LC3 II and P62 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( C ) HeLa cells were infected with EV71 at an MOI of 0.5 in the presence of 1 µM 6-TG. After 24 h, the EV71 VP1, LC3 I, LC3 II and P62 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( D ) HeLa cells were transfected with siBIRC3 or siNC for 30 h, then the cells were infected with EV71 at an MOI of 0.5 for 24 h. The BIRC3, LC3 I, LC3 II, P62 and EV71 VP1 expression in harvested cells was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. Data were pooled from three ( B-D ) or two ( A ) independent experiments, and were shown as the mean ± SEM (ns, no significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001)

Article Snippet: Rabbit anti-BIRC3 antibody Mouse anti-Flag tag antibody , ABclonal (Wuhan, China) Servicebio (Wuhan, China) , A0833 GB15938-100.

Techniques: Infection, Immunostaining, Labeling, Expressing, Western Blot, Software, Transfection

6-TG addition diminished BIRC3-mediated autophagy. ( A ) HeLa cells were incubated with 1 or 3 µM 6-TG for 24 h, the LC3 I, LC3 II, P62 and BIRC3 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( B ) HeLa cells were stimulated with 100 nM rapamycin for 12 h, then they were incubated with 1 µM 6-TG for 24 h. The LC3 I, LC3 II and P62 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( C ) HeLa cells were transfected with 0.5–1 µg pBIRC3 or pVector for 30 h. The cells were harvested and Fag-BIRC3, LC3 I, LC3 II and P62 expression was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( D ) HeLa cells were transfected with siBIRC3 or siNC for 30 h, respectively. The cells were harvested and BIRC3, LC3 I, LC3 II and P62 expression was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. Data were pooled from three ( A-D ) independent experiments, and were shown as the mean ± SEM (ns, no significant, * P < 0.05, ** P < 0.01, *** P < 0.001)

Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet: 6-TG addition diminished BIRC3-mediated autophagy. ( A ) HeLa cells were incubated with 1 or 3 µM 6-TG for 24 h, the LC3 I, LC3 II, P62 and BIRC3 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( B ) HeLa cells were stimulated with 100 nM rapamycin for 12 h, then they were incubated with 1 µM 6-TG for 24 h. The LC3 I, LC3 II and P62 expression was quantified via western blot analysis, normalized against β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( C ) HeLa cells were transfected with 0.5–1 µg pBIRC3 or pVector for 30 h. The cells were harvested and Fag-BIRC3, LC3 I, LC3 II and P62 expression was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. ( D ) HeLa cells were transfected with siBIRC3 or siNC for 30 h, respectively. The cells were harvested and BIRC3, LC3 I, LC3 II and P62 expression was evaluated by western blot assay, normalized to β-actin. The intensity of western blot bands signals of LC3 II/LC3 I and P62/β-actin was quantified by ImageJ software. Data were pooled from three ( A-D ) independent experiments, and were shown as the mean ± SEM (ns, no significant, * P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Rabbit anti-BIRC3 antibody Mouse anti-Flag tag antibody , ABclonal (Wuhan, China) Servicebio (Wuhan, China) , A0833 GB15938-100.

Techniques: Incubation, Expressing, Western Blot, Software, Transfection

Journal: BMC Microbiology

Article Title: 6-thioguanine inhibits EV71 replication by reducing BIRC3-mediated autophagy

doi: 10.1186/s12866-025-03752-8

Figure Lengend Snippet:

Article Snippet: Rabbit anti-BIRC3 antibody Mouse anti-Flag tag antibody , ABclonal (Wuhan, China) Servicebio (Wuhan, China) , A0833 GB15938-100.

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