Journal: Nature Communications
Article Title: Shade-induced RTFL/DVL peptides negatively regulate the shade response by directly interacting with BSKs in Arabidopsis
doi: 10.1038/s41467-023-42618-3
Figure Lengend Snippet: a Pictures of Col-0, bsk3 , bsk6 , 35 S::BSK3ox-Flag (# 4 , #5 ) ( BSK3ox #4, #5 ), and 35 S :: BSK6ox-Flag ( #6, #20 ) ( BSK6ox #6, #20 ) seedlings grown under white light (WL) and low R:FR conditions. b The petiole lengths of the cotyledons in Col-0, bsk3 , bsk6 , BSK3ox ( #4 , #5 ), and BSK6ox ( #6 , #20 ) lines. c The petiole lengths of the first and second leaves in the Col-0, bsk3 , bsk6 , BSK3ox ( #4, #5 ), and BSK6ox ( #6 , #20 ) lines. d Pictures of Col-0, dvl1-1D , dvl1-1D * 35 S::BSK3ox-Flag ( #8 , #16 ) ( dvl1-1D * BSK3ox #8 , #16 ) and dvl1-1D * 35 S::BSK6ox-Flag ( #26 , #25 ) ( dvl1-1D * BSK6ox #26 , #25 ) seedlings grown under WL and low R:FR conditions. e The petiole lengths of the cotyledons in the Col-0, dvl1-1D , dvl1-1D * BSK3ox ( #8 , #16 ), and dvl1-1D * BSK6ox ( #26 , #25 ) seedlings. f The petiole lengths of the first and second leaves in the Col-0, dvl1-1D , dvl1-1D * BSK3ox ( #8 , #16 ), and dvl1-1D * BSK6ox ( #26 , #25 ) seedlings. g , h The petiole lengths of the cotyledons in the Col-0, dvl1-1D , 35 S::RTFL18 ( #7 , #8 ), rtfl18*rtfl21 , bri1-301 , and bsk3 lines treated with 0, 0.1, or 1.0 μM eBL ( g ) or 0, 15, or 30 μM bikinin ( h ). The left panel represents the lengths and the right panel represents the response to eBL ( g ) or bikinin ( h ). In the right panel, the cotyledon petiole lengths in seedlings treated with 0 μM eBL ( g ) or 0 μM bikinin ( h ) were standardized to be “100%”. The asterisks indicate significant differences to Col-0 (Student’s two-sided t test, * P < 0.05, ** P < 0.01, *** P < 0.001, ns indicates no significance). The numbers in the bar charts represent n of each sample. Data are presented as mean values +/− SEM. Letters in b , c , e – h indicate significant differences between mean values (two-way ANOVA: Tukey’s multiple comparisons test, P < 0.01), and groups with the same letters are not significantly different. Scale bars represent 5 mm. Source data are provided as a Source Data file.
Article Snippet: We referred to methods in the literature , and made improvements. eBL (Aladdin, E128317-10mg) and bikinin (Sigma, SML0094-5 MG) were each dissolved to 100 mM in dimethyl sulfoxide as stock solutions and stored at −20 °C.
Techniques:
