Journal: Journal of Ginseng Research
Article Title: Panaxadiol promotes angiogenesis against chronic cerebral hypoperfusion injury through the VEGF-A/p38 MAPK/Src signaling pathway
doi: 10.1016/j.jgr.2025.10.001
Figure Lengend Snippet: PD treatment promotes the cell proliferation, migration, and tube formation in vitro . (A) The bEnd.3 cells viability after PD treatment for 24 h. (B) The cytotoxicity of PD treatment for 24 h. (C and D) Representative immunofluorescence images and quantitative graph of bEnd.3 cells. The ratio of BrdU positive cells was determined by immunofluorescence staining and expressed as a percentage of total cells. Proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). Scale bar: 100 μm. (E and F) Representative transwell migration images and quantitative graph of bEnd.3 cells. Scale bar: 250 μm. (G and H) Representative horizontal migration images and quantitative graph of bEnd.3 cells. The wound healing of bEnd.3 cells was expressed as a percentage of the 16 h wound area compare to 0 h wound area. Scale bar: 150 μm. (I–K) Representative tube formation images and quantitative graph of bEnd.3 cells. Scale bar: 250 μm. One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n is 3–5 in per group, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 vs PD (0 μM) group or control group.
Article Snippet: bEnd.3 cells (ATCC, CRL-2299) were cultured in complete medium at 37 °C with 5 % CO 2 [ ].
Techniques: Migration, In Vitro, Immunofluorescence, Staining, Labeling, Control