bay 876  (MedChemExpress)

Journal: iScience

Article Title: Disulfidptosis in PCOS pathogenesis: Multi-omics identification of LRPPRC as a diagnostic biomarker and therapeutic target

doi: 10.1016/j.isci.2026.116561

Figure Lengend Snippet: Synergistic induction of disulfidptosis in granulosa cells by LRPPRC deficiency and pharmacological GLUT1 inhibition (A) Representative F-actin (red) and DAPI (blue) fluorescence staining images of KGN cells. Cells with control knockdown (Sh NC) or LRPPRC knockdown (Sh LRPPRC) were treated with DMSO (vehicle control) or the GLUT1-specific inhibitor BAY-876 (BAY), and subsequently cultured under glucose-sufficient (+Glc), glucose-deprivation (-Glc), or 2-mercaptoethanol-rescued (2ME/-Glc) conditions. The severe actin cytoskeletal collapse induced by the combination of LRPPRC deficiency, BAY-876, and glucose starvation is completely rescued by the disulfide-reducing agent 2 ME. Images are representative of n = 3 independent biological replicates. Scale bars, 50 μm. (B) Flow cytometry analysis quantifying cell death. Upper and middle panels display representative flow histograms showing the percentage of dead cells (PI-positive population) for the indicated treatment groups under +Glc and -Glc conditions, respectively. The lower panel is a quantitative bar graph summarizing the PI-positive percentage. The results demonstrate a significant synergistic lethal effect when combining LRPPRC knockdown with BAY-876 treatment under glucose deprivation. Data are presented as the mean ± SD of n = 3 independent biological replicates. ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001 for the indicated pairwise comparisons.

Article Snippet: BAY-876 , MedChemExpress , Cat# HY-100017.

Techniques: Inhibition, Fluorescence, Staining, Control, Knockdown, Cell Culture, Flow Cytometry

Journal: Metabolites

Article Title: Lactate Metabolism Dysregulation Drives the Pathogenesis of Acute Kidney Injury

doi: 10.3390/metabo16060434

Figure Lengend Snippet: BAY876 alleviates IRI- and LPS-induced acute kidney injury by inhibiting lactate metabolism. ( A ) Immunofluorescence staining of kidney injury markers (KIM-1, NGAL) in renal tissues from each group (Control, IRI, IRI+BAY876, LPS, LPS+BAY876). ( B , C ) Representative H&E-stained kidney sections and corresponding acute tubular necrosis (ATN) scores in the IRI ( B ) and LPS ( C ) model. ( D – F ) Serum creatinine ( D ), blood urea nitrogen (BUN) ( E ), and lactate ( F ) levels in the IRI model. ( G – I ) Serum levels of inflammatory cytokines IL-1β ( G ), IL-6 ( H ), and TNF-α ( I ) in the IRI model. ( J – L ) Serum creatinine ( J ), BUN ( K ), and lactate ( L ) levels in the LPS model. ( M – O ) Serum levels of inflammatory cytokines IL-1β ( M ), IL-6 ( N ), and TNF-α ( O ) in the LPS model. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001.

Article Snippet: BAY876 (BAY-876, MedChemExpress, Monmouth Junction, NJ, USA, HY-100017), a selective GLUT1 inhibitor, was administered via oral gavage (5 mg/kg) 24 h before surgery.

Techniques: Immunofluorescence, Staining, Control

Journal: Metabolites

Article Title: Lactate Metabolism Dysregulation Drives the Pathogenesis of Acute Kidney Injury

doi: 10.3390/metabo16060434

Figure Lengend Snippet: Inhibition of lactate metabolism mitigates inflammatory response and oxidative stress in renal tubular epithelial cells. ( A , B ) mRNA expression levels of LDHB, IL-6, IL-1β, and TNF-α in HK2 cells treated with increasing concentrations of BAY876 under LPS stimulation ( A ) or hypoxia/reoxygenation (H/R) ( B ). ( C , D ) mRNA expression levels of LDHB, IL-6, IL-1β, and TNF-α in 293T cells treated with BAY876 under LPS ( C ) or H/R ( D ) conditions. ( E , F ) Levels of reactive oxygen species (ROS) in 293T cells treated with BAY876 under LPS ( E ) or H/R ( F ) stimulation. ( G – L ) mRNA expression levels of IL-6 ( G , J ), IL-1β ( H , K ), and TNF-α ( I , L ) in HK2 cells transfected with siLDHB or scramble control, followed by LPS ( G – I ) or H/R ( J – L ) stimulation. ( M , N ) ROS levels in HK2 cells transfected with siLDHB under LPS ( M ) or H/R ( N ) stimulation. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001.

Article Snippet: BAY876 (BAY-876, MedChemExpress, Monmouth Junction, NJ, USA, HY-100017), a selective GLUT1 inhibitor, was administered via oral gavage (5 mg/kg) 24 h before surgery.

Techniques: Inhibition, Expressing, Transfection, Control