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bamhi  (TaKaRa)


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    Structured Review

    TaKaRa bamhi
    Bamhi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14728 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bamhi/BamH+I/10__3390_slash_biom16101439-64-10-11
    Average 99 stars, based on 14728 article reviews
    bamhi - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: ABA-Induced Cargo Proteins Loading in Extracellular Vesicles for Gene Editing.
    Article Snippet: .. The resulting product was digested with BamHI (Takara, Cat#1010, Dalian, China) and ligated into the BamHI-treated pCS2-LbCpf1-3xHA vector [30] using T4 DNA ligase(Thermo Fisher, Cat#EL0011, Waltham, MA, USA)to generate the pCS2-BASP1-PYL1-HA plasmid. ..

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. The purified luxS-eGFP fusion product was double-digested with EcoRI and BamHI (Takara Bio Inc.) at 37 ◦C for 3 h, and the pUC-19 vector was similarly digested at the corresponding sites. .. The digested fragments were ligated into pUC-19 using T4 DNA ligase (Takara Bio Inc., Cat. No. 2011B) following the manufacturer’s protocol.

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Amplification:

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Polymerase Chain Reaction:

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Cloning:

    Article Title: PrimPol toggled by ATR modulates fork slowing and repriming under oncogenic KRAS-induced replication stress
    Article Snippet: .. To establish doxycycline-inducible PrimPol H358 cells, PrimPol-myc-FLAG cDNA was amplified by PCR using Pfu Turbo DNA Polymerase, and inserted into the BamHI and NotI sites of the pENTR GateWay vector using the In-Fusion HD cloning protocol (TaKaRa, Cat# 638948). .. pENTRPrimPol-myc-FLAG was recombined into CSIV-TRE-RfA-UbC-Puro (a kind gift from Dr. Nakanishi) using the GateWay system (Invitrogen, Cat# 11791-020).

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Incubation:

    Article Title: Age-dependent accumulation of RAD51 on non-damaged chromosomes prevents chromosome segregation in mammalian oocytes
    Article Snippet: .. When indicated, BamHI (15 units, TaKaRa) was added and the reaction was further incubated for an additional 5 min. ..

    other:


    Purification:

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. The purified luxS-eGFP fusion product was double-digested with EcoRI and BamHI (Takara Bio Inc.) at 37 ◦C for 3 h, and the pUC-19 vector was similarly digested at the corresponding sites. .. The digested fragments were ligated into pUC-19 using T4 DNA ligase (Takara Bio Inc., Cat. No. 2011B) following the manufacturer’s protocol.

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Expressing:

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Agarose Gel Electrophoresis:

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Positive Control:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Over Expression:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Construct:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Sequencing:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..



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