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kpni und bamhi  (TaKaRa)


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  • 99

    Structured Review

    TaKaRa kpni und bamhi
    Kpni Und Bamhi, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14661 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bamhi/BamH+I/pm42127640-51-19-17
    Average 99 stars, based on 14661 article reviews
    kpni und bamhi - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: ABA-Induced Cargo Proteins Loading in Extracellular Vesicles for Gene Editing.
    Article Snippet: .. The resulting product was digested with BamHI (Takara, Cat#1010, Dalian, China) and ligated into the BamHI-treated pCS2-LbCpf1-3xHA vector [30] using T4 DNA ligase(Thermo Fisher, Cat#EL0011, Waltham, MA, USA)to generate the pCS2-BASP1-PYL1-HA plasmid. ..

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Incubation:

    Article Title: Age-dependent accumulation of RAD51 on non-damaged chromosomes prevents chromosome segregation in mammalian oocytes
    Article Snippet: .. When indicated, BamHI (15 units, TaKaRa) was added and the reaction was further incubated for an additional 5 min. ..

    Amplification:

    Article Title: Two ferric uptake regulator proteins in Ralstonia pseudosolanacearum strain OE1-1 function cooperatively in response to the extracellular iron level under ferrous iron-rich conditions
    Article Snippet: .. Amplified delta-fur1-1 and delta-fur1-2 sequences, along with primers delta-fur1-1-FW and delta-fur1-2-RV, were used for a PCR amplification of a 1,384 bp DNA fragment, which was then digested with BamHI (Takara Bio, Ohtsu, Japan) and HindIII (Takara Bio). ..

    Article Title: Two ferric uptake regulator proteins in Ralstonia pseudosolanacearum strain OE1-1 function cooperatively in response to the extracellular iron level under ferrous iron-rich conditions.
    Article Snippet: .. Amplified delta-fur1-1 and delta-fur1-2 sequences, along with primers delta-fur1-1-FW Month XXXX Volume 0 Issue 0 10.1128/aem.02276-2511 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a em o n 08 M ar ch 2 02 6 by 2 a0 9: ba c5 :5 5f c: 1d 0f ::2 e5 :5 c. and delta-fur1-2-RV, were used for a PCR amplification of a 1,384 bp DNA fragment, which was then digested with BamHI (Takara Bio, Ohtsu, Japan) and HindIII (Takara Bio). ..

    Polymerase Chain Reaction:

    Article Title: Two ferric uptake regulator proteins in Ralstonia pseudosolanacearum strain OE1-1 function cooperatively in response to the extracellular iron level under ferrous iron-rich conditions
    Article Snippet: .. Amplified delta-fur1-1 and delta-fur1-2 sequences, along with primers delta-fur1-1-FW and delta-fur1-2-RV, were used for a PCR amplification of a 1,384 bp DNA fragment, which was then digested with BamHI (Takara Bio, Ohtsu, Japan) and HindIII (Takara Bio). ..

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Article Title: Two ferric uptake regulator proteins in Ralstonia pseudosolanacearum strain OE1-1 function cooperatively in response to the extracellular iron level under ferrous iron-rich conditions.
    Article Snippet: .. Amplified delta-fur1-1 and delta-fur1-2 sequences, along with primers delta-fur1-1-FW Month XXXX Volume 0 Issue 0 10.1128/aem.02276-2511 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a em o n 08 M ar ch 2 02 6 by 2 a0 9: ba c5 :5 5f c: 1d 0f ::2 e5 :5 c. and delta-fur1-2-RV, were used for a PCR amplification of a 1,384 bp DNA fragment, which was then digested with BamHI (Takara Bio, Ohtsu, Japan) and HindIII (Takara Bio). ..

    other:


    Purification:

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Expressing:

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Agarose Gel Electrophoresis:

    Article Title: Analysis of metabolic rewiring in MDR1-overexpressing drug-resistant glioblastoma
    Article Snippet: The amplified PCR product was purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel, Germany, 740609.50) according to the manufacturer’s protocol. .. The purified PCR product and plvxIRES Puro lenti expression vector were digested with BamHI (Takara, Japan, 1,605) and XhoI (Takara, Japan, Cat No #1635) restriction enzymes and resolved by agarose gel electrophoresis. .. The gel-purified insert and vector were ligated in a 3:1 insert-to-vector molar ratio using T4 DNA ligase (Takara, Japan, 2011A) and incubated at 16 °C for 2 h. The ligation mixture was transformed into E. coli DH5α competent cells.

    Positive Control:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Over Expression:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Construct:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Cloning:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..

    Sequencing:

    Article Title: Genome-Wide CRISPR Screening Identifies Genetic Modulators of Amyloid Precursor Protein Processing
    Article Snippet: .. For the positive control of amyloidogenic processing, a human BACE1 overexpression vector was constructed by cloning the BACE1 coding sequence into the pHAGE-EF1α-Blast backbone using AgeI (NEB, #R3552) and BamHI (Takara, #1605) restriction sites. ..



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