bacteria escherichia coli  (ATCC)

Journal: Materials Today Bio

Article Title: MnO x -armored magnesium implants for anti-osteosarcoma and biofilm eradication by charge-transfer interference

doi: 10.1016/j.mtbio.2026.102817

Figure Lengend Snippet: Antibacterial tests of various samples with and without NIR irradiation (1.00 W cm −2 , 5 min). (A) Photos of bacterial colonies and (B) (i, v) the corresponding antibacterial rates, (ii, vi) absorbance values, (ii, vii) cell membrane potential and (iv, vii) intracellular ROS against E. coli and S. aureus inoculated on sample surfaces. (C) SEM image of bacteria on the sample surface surfaces. (n = 3, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001).

Article Snippet: The antibacterial properties of the samples were evaluated using Gram-negative bacteria Escherichia coli ( E. coli, ATCC 35218) and Gram-positive bacteria S. aureus (ATCC 43300 ).

Techniques: Irradiation, Membrane, Bacteria

Journal: Nature Communications

Article Title: TCL1A mediates DNA methylation defects in recurrent hydatidiform mole with NLRP7 pathogenic variants

doi: 10.1038/s41467-026-69744-y

Figure Lengend Snippet: a Imaging of endogenous TCL1A in the human abnormal oocytes/embryos. Blocked, a pre-blocked TCL1A antibody. The total number of oocytes/embryos in 3 independent experiments is indicated. Scale bar, 20 μm. b Relative mean fluorescence intensity of TCL1A and NLRP7 in nucleus (nc) and subcortex (sc) of GV oocytes and blastomeres. The subcortical fluorescence intensity in GV-stage oocytes was set to 1.0. Error bars, SEM. P -values by an unpaired two-tailed Student’s t -test. c Live-cell imaging of GFP-TCL1A in mouse GV oocytes injected with GFP-TCL1A and NLRP7 variant-mCherry mRNAs. The proportion of oocytes exhibiting a similar GFP pattern is indicated. Scale bar, 20 μm. N ≥ 2 replicates for each group. d Co-IP assays detecting the interactions between TCL1A and human DNMT1, DMT3A, DNMT3B3, or DNMT3L. N = 3 independent replicates. e An assay detecting the effect of TCL1A on the DNMT3A activity. The activity of DNMT3A was set to 1.0. Error bars, SEM. N = 3 independent replicates. P -values by an unpaired two-tailed Student’s t -test. f Co-IP assays detecting the effects of overexpressed TCL1A on DNMT3A-DNMT3B3 or DNMT3A-DNMT3L formation. g Generating dual-inducible mESCs and inducing them into fPSCs. S1, Shield1; Dox, doxycycline. See the Methods section for details. h Violin plots displaying the global DNA methylation levels in the four polyclonal fPSCs. N = 5327 bins. The box spans the 25th–75th percentiles, with whiskers indicating 1.5× interquartile range. Central lines, the median. P -values by an unpaired two-tailed Student’s t -test. i Cumulative bar plot of the methylation levels. High, medium, and low methylation levels are defined as >75%, 10–50%, and <10%, respectively. j The box plots showing the DNA methylation levels in genomic Features. The number of each features is indicated. The box spans the 25th–75th percentiles, with whiskers indicating 1.5× interquartile range. Central lines, the median. k A hypothetical model illustrating how pathogenic NLRP7 variants lead to the failure of de novo DNA methylation in oocytes. PDB: hSCMCcore (8X7V); DNMT3A (6PA7). The image was created in BioRender ( https://BioRender.com/lh55y4f ).

Article Snippet: The genomic DNA of fPSCs cultured in 6-well plates was isolated with a DNA isolation mini kit (Vazyme, DC112).

Techniques: Imaging, Fluorescence, Two Tailed Test, Live Cell Imaging, Injection, Variant Assay, Co-Immunoprecipitation Assay, Activity Assay, DNA Methylation Assay, Methylation