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myeloperoxidase mpo inhibitor azd5904  (MedChemExpress)


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    Structured Review

    MedChemExpress myeloperoxidase mpo inhibitor azd5904
    Myeloperoxidase Mpo Inhibitor Azd5904, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/azd5904/AZD5904/pm41698066-116-33-42
    Average 94 stars, based on 18 article reviews
    myeloperoxidase mpo inhibitor azd5904 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Control:

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies.
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Blocking Assay:

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies.
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Activity Assay:

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies.
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Activation Assay:

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies.
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies
    Article Snippet: The macrophages were labeled with an anti-CD68 antibody (565594; BD Biosciences, Franklin Lakes, USA) and identified via flow cytometry. .. To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively. ..

    Mouse Assay:

    Article Title: Small-molecule myeloperoxidase (MPO) inhibition prevents neutrophil entry into the meninges and improves late spatial memory deficits in a murine model of delayed cerebral injury (DCI) after subarachnoid hemorrhage (SAH)
    Article Snippet: .. Behavior- Mice were randomized to receive either AZD5904 (MedChemExpress, Monmouth Junction, NJ), a non-reversable, small molecule MPO inhibitor (MPOi) (20 μmol/kg in phosphate buffered saline in dimethyl sulfoxide (PBS/DMSO)) or PBS/DMSO (placebo) given by the intraperitoneal route (IP). ..

    Saline:

    Article Title: Small-molecule myeloperoxidase (MPO) inhibition prevents neutrophil entry into the meninges and improves late spatial memory deficits in a murine model of delayed cerebral injury (DCI) after subarachnoid hemorrhage (SAH)
    Article Snippet: .. Behavior- Mice were randomized to receive either AZD5904 (MedChemExpress, Monmouth Junction, NJ), a non-reversable, small molecule MPO inhibitor (MPOi) (20 μmol/kg in phosphate buffered saline in dimethyl sulfoxide (PBS/DMSO)) or PBS/DMSO (placebo) given by the intraperitoneal route (IP). ..



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    Inhibition of JNK phosphorylation reduces ANCA-induced inflammasome activation (A) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then incubated with control IgG or MPO-ANCA IgG (500 μg/mL) for 1 h. Representative western blot images and quantification were used to examine the MAPK signaling pathway in macrophages incubated with control IgG or MPO-ANCA IgG. (B,C) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the JNK signaling pathway inhibitor <t>SP600125</t> (20 μM, 1 h prior to ANCAs). (B) Representative western blot images and quantification of phosphorylated JNK, NLRP3, cleaved caspase-1 and N-terminal GSDMD expressions. (C) Real-time PCR analysis of IL1B mRNA levels in PBMC-derived macrophages was used to evaluate the effects of the JNK inhibitor SP600125 on ANCA-induced pyroptosis. (D–F) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the MPO inhibitor AZD5904 (20 μM, 1 h prior to ANCAs). (D) Representative western blot images and quantification of phosphorylated JNK demonstrated that an MPO-specific inhibitor reduced ANCA-induced JNK phosphorylation. (E) PBMC-derived macrophage death was assessed by PI staining (100× magnification). (F) PBMC-derived macrophage death was assessed by measuring LDH levels in culture supernatants, as determined by LDH cytotoxicity assays. Data are expressed as the mean ± SD of at least three independent experiments. P: phosphorylated. #P < 0.05, ##P < 0.01, ###P < 0.001.
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    Inhibition of JNK phosphorylation reduces ANCA-induced inflammasome activation (A) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then incubated with control IgG or MPO-ANCA IgG (500 μg/mL) for 1 h. Representative western blot images and quantification were used to examine the MAPK signaling pathway in macrophages incubated with control IgG or MPO-ANCA IgG. (B,C) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the JNK signaling pathway inhibitor <t>SP600125</t> (20 μM, 1 h prior to ANCAs). (B) Representative western blot images and quantification of phosphorylated JNK, NLRP3, cleaved caspase-1 and N-terminal GSDMD expressions. (C) Real-time PCR analysis of IL1B mRNA levels in PBMC-derived macrophages was used to evaluate the effects of the JNK inhibitor SP600125 on ANCA-induced pyroptosis. (D–F) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the MPO inhibitor AZD5904 (20 μM, 1 h prior to ANCAs). (D) Representative western blot images and quantification of phosphorylated JNK demonstrated that an MPO-specific inhibitor reduced ANCA-induced JNK phosphorylation. (E) PBMC-derived macrophage death was assessed by PI staining (100× magnification). (F) PBMC-derived macrophage death was assessed by measuring LDH levels in culture supernatants, as determined by LDH cytotoxicity assays. Data are expressed as the mean ± SD of at least three independent experiments. P: phosphorylated. #P < 0.05, ##P < 0.01, ###P < 0.001.
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    Image Search Results


    Inhibition of JNK phosphorylation reduces ANCA-induced inflammasome activation (A) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then incubated with control IgG or MPO-ANCA IgG (500 μg/mL) for 1 h. Representative western blot images and quantification were used to examine the MAPK signaling pathway in macrophages incubated with control IgG or MPO-ANCA IgG. (B,C) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the JNK signaling pathway inhibitor SP600125 (20 μM, 1 h prior to ANCAs). (B) Representative western blot images and quantification of phosphorylated JNK, NLRP3, cleaved caspase-1 and N-terminal GSDMD expressions. (C) Real-time PCR analysis of IL1B mRNA levels in PBMC-derived macrophages was used to evaluate the effects of the JNK inhibitor SP600125 on ANCA-induced pyroptosis. (D–F) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the MPO inhibitor AZD5904 (20 μM, 1 h prior to ANCAs). (D) Representative western blot images and quantification of phosphorylated JNK demonstrated that an MPO-specific inhibitor reduced ANCA-induced JNK phosphorylation. (E) PBMC-derived macrophage death was assessed by PI staining (100× magnification). (F) PBMC-derived macrophage death was assessed by measuring LDH levels in culture supernatants, as determined by LDH cytotoxicity assays. Data are expressed as the mean ± SD of at least three independent experiments. P: phosphorylated. #P < 0.05, ##P < 0.01, ###P < 0.001.

    Journal: Acta Biochimica et Biophysica Sinica

    Article Title: NLRP3 inflammasome activity and pyroptosis are involved in CD206 + macrophage activation by MPO anti-neutrophil cytoplasmic antibodies

    doi: 10.3724/abbs.2025080

    Figure Lengend Snippet: Inhibition of JNK phosphorylation reduces ANCA-induced inflammasome activation (A) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then incubated with control IgG or MPO-ANCA IgG (500 μg/mL) for 1 h. Representative western blot images and quantification were used to examine the MAPK signaling pathway in macrophages incubated with control IgG or MPO-ANCA IgG. (B,C) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the JNK signaling pathway inhibitor SP600125 (20 μM, 1 h prior to ANCAs). (B) Representative western blot images and quantification of phosphorylated JNK, NLRP3, cleaved caspase-1 and N-terminal GSDMD expressions. (C) Real-time PCR analysis of IL1B mRNA levels in PBMC-derived macrophages was used to evaluate the effects of the JNK inhibitor SP600125 on ANCA-induced pyroptosis. (D–F) PBMC-derived macrophages were pretreated with LPS (100 ng/mL) for 5 h and then with control IgG or MPO-ANCA IgG with or without the MPO inhibitor AZD5904 (20 μM, 1 h prior to ANCAs). (D) Representative western blot images and quantification of phosphorylated JNK demonstrated that an MPO-specific inhibitor reduced ANCA-induced JNK phosphorylation. (E) PBMC-derived macrophage death was assessed by PI staining (100× magnification). (F) PBMC-derived macrophage death was assessed by measuring LDH levels in culture supernatants, as determined by LDH cytotoxicity assays. Data are expressed as the mean ± SD of at least three independent experiments. P: phosphorylated. #P < 0.05, ##P < 0.01, ###P < 0.001.

    Article Snippet: To investigate the effect of ANCA on macrophages, adherent PBMC-derived macrophages were pretreated with 100 ng/mL lipopolysaccharide (LPS) (L2630; Sigma-Aldrich) for 5 h, followed by exposure to 500 μg/mL MPO-ANCA IgG or control IgG for 1 h. In the different experimental groups, AZD5904, MCC950 or SP600125 (HY-111341, HY-12815 and HY-12041; MedChemExpress, Monmouth Junction, USA) was added 1 h before the addition of IgG to block MPO activity and inhibit NLRP3 activation or the JNK signaling pathway, respectively.

    Techniques: Inhibition, Phospho-proteomics, Activation Assay, Derivative Assay, Incubation, Control, Western Blot, Real-time Polymerase Chain Reaction, Staining