au  (ATCC)

Journal: Molecular Biology Reports

Article Title: Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂–COX-2–NF-κB pathway

doi: 10.1007/s11033-026-11835-6

Figure Lengend Snippet: Viability of HER2-positive breast cancer and normal cell lines following treatment with ibuprofen and nimesulide derivatives. Cell viability was assessed by WST-1 assay after 72 h of treatment with selected ibuprofen and nimesulide derivatives in HER2-positive AU565 and SKBR3 breast cancer cells and normal HDF and MCF-12 A cells. ( a ) AU565 breast cancer cells. ( b ) HDF fibroblast cells. ( c ) SKBR3 breast cancer cells. ( d ) MCF-12 A mammary epithelial cells. Data are presented as mean ± SD from three independent experiments (**** P ≤ 0.0001 vs. control)

Article Snippet: AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described previously [ ].

Techniques: WST-1 Assay, Control

Journal: Molecular Biology Reports

Article Title: Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂–COX-2–NF-κB pathway

doi: 10.1007/s11033-026-11835-6

Figure Lengend Snippet: Effects of ibuprofen and nimesulide derivatives on cell cycle distribution in AU565 and SKBR3 cells. ( a ) AU565 and ( b ) SKBR3 cells were treated with D1 , D7 , or D8 for 24 h, and cell cycle phase distributions were determined by Cytell Cell Imaging System (GE Healthcare, UK). Data are presented as mean ± SD from three independent experiments (* P ≤ 0.05, *** P ≤ 0.001, **** P ≤ 0.0001 vs. control)

Article Snippet: AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described previously [ ].

Techniques: Imaging, Control

Journal: Molecular Biology Reports

Article Title: Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂–COX-2–NF-κB pathway

doi: 10.1007/s11033-026-11835-6

Figure Lengend Snippet: Induction of apoptosis and caspase-3/7 activation by ibuprofen and nimesulide derivatives in HER2-positive breast cancer cells. Apoptosis was evaluated in AU565 and SKBR3 cells following 72 h of treatment with D1 , D7 , or D8 . Annexin V–FITC/7-AAD staining was used to quantify apoptotic cell populations by Guava ® Muse ® Cell Analyzer (Merck, Germany). ( a ) Representative dot plots showing the apoptotic profiles of control, vehicle control and drug-treated AU565 and SKBR3 cells. Distribution of viable, early-apoptotic, late-apoptotic, and necrotic cell fractions in AU565 ( b ) and SKBR3 ( c ) cells. Caspase-3/7 activity was measured by colorimetric assay in AU565 ( d ) cells at 24 and 48 h, and in SKBR3 ( e ) cells at 24, 36, and 48 h. Results are expressed as fold-change relative to vehicle-treated controls. Data are presented as mean ± SD from three independent experiments (** P ≤ 0.01, **** P ≤ 0.0001 vs. control)

Article Snippet: AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described previously [ ].

Techniques: Activation Assay, Staining, Control, Activity Assay, Colorimetric Assay

Journal: Molecular Biology Reports

Article Title: Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂–COX-2–NF-κB pathway

doi: 10.1007/s11033-026-11835-6

Figure Lengend Snippet: Relative mRNA expression of PLA2G2A and PTGS2 in HER2-positive breast cancer cells following treatment with ibuprofen and nimesulide derivatives. Quantitative real-time PCR (qRT-PCR) analysis was performed on AU565 and SKBR3 cells after 24 h exposure to D1 , D7 , and D8 . ( a ) PLA2G2A expression and ( b ) PTGS2 expression were determined relative to vehicle-treated controls. Gene expression values were normalized to 18sRNA housekeeping gene. Data are presented as mean ± SD from three independent experiments (**** P ≤ 0.0001 vs. control)

Article Snippet: AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described previously [ ].

Techniques: Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Gene Expression, Control

Journal: Molecular Biology Reports

Article Title: Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂–COX-2–NF-κB pathway

doi: 10.1007/s11033-026-11835-6

Figure Lengend Snippet: Inhibition of NF-κB nuclear translocation by ibuprofen and nimesulide derivatives in HER2-positive breast cancer cells. Immunocytochemical analysis of NF-κB localization was performed in AU565 and SKBR3 cells after 24 h treatment with D1 , D7 , and D8 . ( a ) Representative confocal fluorescence images showing NF-κB (green, FITC channel) and nuclear counterstaining with PI (red, CY3 channel). Violin plots showing the nuclear fraction of NF-κB fluorescence intensity in AU565 ( b ) and SKBR3 ( c ) cells, quantified on a single-cell basis ( n ≥ 100 cells per condition, pooled from three independent experiments). Scale bar = 20 μm

Article Snippet: AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described previously [ ].

Techniques: Inhibition, Translocation Assay, Fluorescence, Single Cell

Journal: Molecular Biology Reports

Article Title: Ibuprofen and nimesulide derivatives selectively induce apoptosis in HER2-positive breast cancer via inhibition of the PLA₂–COX-2–NF-κB pathway

doi: 10.1007/s11033-026-11835-6

Figure Lengend Snippet: Reduction of intracellular ROS generation by ibuprofen and nimesulide derivatives. ( a ) Representative fluorescence microscopy images for DCFDA-based detection of reactive oxygen species in AU565 and SKBR3 cells treated for 24 h with D1 , D7 , and D8 . ( b ) Quantitative fluorometric evaluation of intracellular ROS expressed as fold change compared to control in AU565 and SKBR3 cells. Scale bar = 100 μm. Data are presented as mean ± SD from three independent experiments (**** P ≤ 0.0001 vs. control)

Article Snippet: AU565 (CRL-2351, ATCC), SKBR3 (HTB-30, ATCC), HDF (CS-201-012, ATCC) and MCF-12A (CRL-3598, ATCC) cells were cultured in RPMI-1640 as described previously [ ].

Techniques: Fluorescence, Microscopy, Control