Journal: Nature Communications
Article Title: MCARE enhances SERCA1 activity in fast-twitch muscle to maintain calcium handling and muscle integrity
doi: 10.1038/s41467-025-67358-4
Figure Lengend Snippet: In WT muscle fibers, MCARE enhances SERCA1 activity by displacing its inhibitory micropeptide MRLN, thereby maintaining Ca 2+ homeostasis. In contrast, Mcare -deficient fast-twitch fibers exhibit impaired Ca 2+ reuptake, leading to Ca 2+ homeostasis disruption. This dysfunction results in muscle atrophy, rippling contractions, and increased vulnerability to physical stress.
Article Snippet: The membrane was blocked with 5% BSA (Fujifilm Wako Chemicals) for 60 min at RT and incubated overnight at 4 °C with primary antibody, anti-MCARE antibody (1:1,000, generated by immunizing rabbits with the peptide CPEAYTEDDKTEED), anti-Caveolin-3 antibody (1:200, Santa Cruz Biotechnology, Cat. No. sc-5310), anti-N-Cadherin antibody (1:1000, Cell Signaling Technology, Cat. No. 4061), anti-Lamin A/C antibody (1:1000, Cell Signaling Technology, Cat. No. 2032), anti-Tom20 antibody (1:1000, Cell Signaling Technology, Cat. No. 42406), anti-GM130 antibody (1:1000, BD Bioscience, Cat. No. 610823) and anti-HSP90 antibody (1:200, Santa Cruz Biotechnology, Cat. No. sc-13119), or incubated for 60 min at RT with other primary antibodies, anti-RyR antibody (1:300, Santa Cruz Biotechnology, Cat. No. sc-376507), anti-SERCA1 antibody (1:1000, Cell Signaling Technology, Cat. No. 4219), anti-SERCA2 antibody (1:200, Santa Cruz Biotechnology, Cat. No. sc-376235), anti-FLAG M2 antibody (1:2000, Sigma-Aldrich, Cat. No. F3165), anti-Myc antibody (1:1000, Sigma-Aldrich, Cat. No. C3956), anti-GFP antibody (1:1000, Cell Signaling Technology, Cat. No. 2956) and anti-GAPDH antibody (1:5000, Proteintech.
Techniques: Activity Assay, Disruption
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