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resource source identifier antibodies gfp proteintech  (Proteintech)


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    Structured Review

    Proteintech resource source identifier antibodies gfp proteintech
    Resource Source Identifier Antibodies Gfp Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atg7/ATG7+Antibody/pm41932333-276-2-7
    Average 96 stars, based on 284 article reviews
    resource source identifier antibodies gfp proteintech - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Ubiquitin Proteomics:

    Article Title: OTUD3-mediated deubiquitination licenses TEX264 to orchestrate ER-phagy for KDM5B degradation in teniposide lung cancer therapy.
    Article Snippet: High expression of Lysine-Specific Demethylase 5B (KDM5B) in lung cancer drives tumorigenesis and immunosuppression.. KDM5B is negatively correlated with endoplasmic reticulum (ER)-phagy receptors such as TEX264, indicating that selective induction of ER-phagy may degrade KDM5B.. Our work revealed that chemotherapeutic drug Teniposide (Ten) was a potent anti-lung cancer agent, which could increase the stability of TEX264.

    Saline:

    Article Title: KDM6B induces demethylation of H3K27me3 in MFN1 to modulate aberrant mitophagy in sepsis-induced acute lung injury.
    Article Snippet: Proteins were transferred onto polyvinylidene difluoride membranes (Cat. No. E801-01; Vazyme Biotech Co., Ltd.). .. The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 for 1 h. Membranes were then incubated with primary antibodies against H3K27me3 (ab192985, Abcam, Cambridge, MA, USA), H3 (17168-1-AP, Proteintech, Wuhan, China), MFN1 (13798-1-AP, Proteintech), MFN2 (12186-1-AP, Proteintech), Drp1 (12957-1-AP, Proteintech), ATG7 (10088-2-AP, Proteintech), PINK1 (23274-1-AP, Proteintech), Parkin (14060-1-AP, Proteintech), TOM20 (11802-1-AP, Proteintech), LC3 (14600- 1-AP, Proteintech), and GAPDH (10494-1-AP, Proteintech) at 4 °C overnight. .. Subsequently, membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (ab6721, Abcam) at room temperature for 1 h. Protein bands were visualized using a BeyoECL chemiluminescence detection system (Cat. No. P0018S; Beyotime Biotechnology).

    Incubation:

    Article Title: KDM6B induces demethylation of H3K27me3 in MFN1 to modulate aberrant mitophagy in sepsis-induced acute lung injury.
    Article Snippet: Proteins were transferred onto polyvinylidene difluoride membranes (Cat. No. E801-01; Vazyme Biotech Co., Ltd.). .. The membranes were blocked with 5% skim milk in Tris-buffered saline with Tween 20 for 1 h. Membranes were then incubated with primary antibodies against H3K27me3 (ab192985, Abcam, Cambridge, MA, USA), H3 (17168-1-AP, Proteintech, Wuhan, China), MFN1 (13798-1-AP, Proteintech), MFN2 (12186-1-AP, Proteintech), Drp1 (12957-1-AP, Proteintech), ATG7 (10088-2-AP, Proteintech), PINK1 (23274-1-AP, Proteintech), Parkin (14060-1-AP, Proteintech), TOM20 (11802-1-AP, Proteintech), LC3 (14600- 1-AP, Proteintech), and GAPDH (10494-1-AP, Proteintech) at 4 °C overnight. .. Subsequently, membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (ab6721, Abcam) at room temperature for 1 h. Protein bands were visualized using a BeyoECL chemiluminescence detection system (Cat. No. P0018S; Beyotime Biotechnology).

    Article Title: Vericiguat Alleviates Atrial Electrical and Structural Remodeling in Rats with Atrial Fibrillation
    Article Snippet: Proteins were then transferred to PVDF membranes (Millipore, USA) at 200 mA for 90 minutes. .. Membranes were blocked with a protein-free rapid sealing solution (PS108, Epizyme) and incubated overnight at 4°C with primary antibodies against: Collagen I (1:500, AF7001, Affinity), CaMKII (1:4000, 12666-2-AP, Proteintech), Cx43 (1:4000, 26980-1-AP, Proteintech), ATG7 (1:1000, ET1610-53, HuaAn), P62 (1:1000, A7785, ABclonal), LC3B (1:2000, ab192890, Abcam), GAPDH (1:30,000, AC001, ABclonal), and Tubulin (1:3000, 11224-1- AP; Proteintech). .. After washing with Tris-buffered saline with Tween 20, membranes were incubated with horseradish peroxidase–conjugated goat anti-rabbit IgG (1:10,000, bs-0295G-HRP, Bioss) for 1 hour at room temperature.

    Article Title: Disruption of FGL2 induces TFEB-dependent lysosomal degradation of PD-L1 and enhances the efficacy of anti-PD1 therapy in hepatocellular carcinoma
    Article Snippet: .. Next, membranes were incubated with the following antibodies: FGL2 (1:500) (sc-100276, Santa Cruz Biotechnology), PD-L1 (1:4000) (66248-1-Ig, Proteintech), DDDDK-Tag (1:5000) (AE063, Abclonal), LAMP1 (1:10000) (67300-1-Ig, Proteintech), CTSD (1:10000) (21327-1-AP, Proteintech), TFEB (1:5000) (68632-1-Ig, Proteintech), phospho-TFEB (1:1000) (37681, CST), TFE3 (1:500) (A7936, Abclonal), MITF (1:1000) (13092-1-AP, Proteintech), GAPDH (1:50000) (60004-1-Ig, Proteintech), Histone H3 (1:5000) (17168-1-AP, Proteintech), p70S6K (1:1000) (2708, CST), phospho-p70S6K (1:1000) (9234, CST), 4EBP1 (1:1000) (9644, CST), phospho-4EBP1 (1:1000) (2855, CST), AKT (1:1000) (9272, CST), phospho-AKT (1:1000) (4060, CST), GSK3β(1:1000) (12456, CST), phospho-GSK3β (1:1000) (5558, CST), PKC (1:4000) (12919-1-AP, Proteintech), phospho-PKC (1:1000) (38938, CST), ERK1/2 (1:1000) (16443-1-AP, Proteintech), phospho-p44/42 MAPK (Erk1/2) (1:2000) (4370, CST), ATG5 (1:2000) (10181-2-AP, Proteintech), ATG7 (1:1000) (10088-2-AP, Proteintech), HRP goat anti-rabbit IgG (1:10000) (A21020, Abbkine) and HRP goat anti-mouse IgG (1:10000) (A21010, Abbkine). ..

    Western Blot:

    Article Title: VDAC1 protein derived from extracellular vesicles promotes paclitaxel resistance in gastric cancer through autophagy and mitophagy
    Article Snippet: .. The primary antibodies used in western blots were as follows: β-actin (1:1000, T002; Affinity, Cincinnati, OH, USA); GAPDH (1:5000, 104941-AP; Proteintech, Wuhan, China); P-gp (1:3000, 22336-1-AP; Proteintech); CD9 (1:1000, sc-13118; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), HSP90 (1:5000, 13171-1-AP; Proteintech); calnexin (1:1000; c-23954; Santa Cruz Biotechnology, Inc. USA); TSG101 (1:1000, c-7964; Santa Cruz Biotechnology, Inc.); VDAC1 (1:1000, PTM-6157; PTM Biolabs, Chicago, IL, USA); p62 (1:1000, #5114; Cell Signaling Technology, Danvers, Massachusetts, USA); LC3B (1:1000, L7543; Sigma); ULK1 (1:1000, 20986-1-AP; Proteintech); p-ULK1 (1:1000, #14202; Cell Signaling Technology); AMPK (1:1000, #5831; Cell Signaling Technology); p-AMPK (1:1000, #2535; Cell Signaling Technology); mTOR (1:5000, 20657-1-AP; Proteintech); p-mTOR (1:2000, 67778-1-Ig; Proteintech); p70S6K (1:2000, 14485-1-AP; Proteintech); p-p70S6K (1:1000, #AF3228; Affinity); ATG5 (1:1000, 10181-2-AP; Proteintech); ATG7 (1:1000, 10088-2-AP; Proteintech); HTRA1 (1:1000, 55011-1-AP, Proteintech); and STEAP4 (1:1000, 11944-1-AP; Proteintech). .. Cells pretreated under specified conditions were harvested and plated in 96-well plates (Corning, NY, USA) at a density of 4000 cells per well.



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