Journal: Cell Reports Methods
Article Title: A 3D multi-compartment assembloid to study combined immune cell infiltration and cytotoxicity
doi: 10.1016/j.crmeth.2026.101307
Figure Lengend Snippet: Collagen-rich environments restrict CAR T cell migration, limiting cytotoxicity (A) Trajectories of CAR T cells embedded in 2 mg/mL (low) and 4 mg/mL (high) collagen gels. Scale bars, 50 μm. (B and C) Speed and displacement of CAR T cells seeded at low and high collagen densities. N = 3, n = 50 cells, donors = 2. Data are mean ± SEM. Statistical test: unpaired t test, ∗∗∗∗ p < 0.0001. (D) Cartoon depicting an assembloid with PDAC cells in the core surrounded by a stromal compartment composed of a low or high density of collagen. CAR T cells seeded in medium on top of the bulk matrix can more readily invade compartments composed of a low collagen density compared to a high collagen density. (E). ASPC1 cells (gold) and CAR T cells (green) in medium coculture of varying collagen densities. Images are maximum intensity projections of stacks of fluorescence confocal images. 10X magnification. Scale bars, 250 μm. (F) Cytotoxicity of CAR T cells against PDAC 3D in varying collagen densities of the medium coculture. E:T ratio, 1. N = 3, n = 3+, donors = 2. Data are mean ± SEM. Statistical test: unpaired t test, ∗∗∗∗ p < 0.0001.
Article Snippet: OVCAR3 ovarian cancer cells (ATCC HTB-161), MDA-MB-231 breast cancer cells (ATCC HTB-26), JAR gestational trophoblastic neoplasia cells (ATCC HTB-144), 293T (ATCC CRL-3216) as well as PANC-1 and ASPC1 pancreatic ductal adenocarcinoma cells (ATCC CRL-1469) were cultured according to ATCC recommendations.
Techniques: Migration, Fluorescence
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