Journal: Advanced Science
Article Title: Artesunate Ameliorates APAP‐induced Liver Injury by Promoting NEDD4L‐Mediated Ubiquitination and Degradation of TXNIP
doi: 10.1002/advs.202521818
Figure Lengend Snippet: Artesunate (ART) alleviates APAP‐induced liver injury. (A) Schematic diagram of the experimental procedure. The mice were fasted for 16 h, then intraperitoneally injected with 400 mg/kg APAP, and 1 h later, they were injected with ART. The samples were collected 24 h later (n = 8). (B,C) The levels of ALT and AST in the serum of the mice (n = 8). (D–F) The levels of TNF‐α, IL‐6, and IL‐1β in the serum of the mice (n = 8). (G) LDH levels in the serum of the mice (n = 8). (H) Representative images of H&E and TUNEL staining of the livers of the mice. (I) Quantification of necrotic area in the livers of the mice (n = 8). (J) Schematic diagram of the experimental procedure. The mice were fasted for 16 h, then intraperitoneally injected with 400 mg/kg APAP, and 10 h later, they were injected with ART. The samples were collected 24 h later (n = 8). (K,L) The levels of ALT and AST in the serum of the mice (n = 8). (M) Representative images of HE‐stained livers from the mice. (N) Quantification of the necrotic area in the livers of the mice (n = 8). (O) LDH levels in the serum of the mice (n = 8). (P) Schematic diagram of the experimental procedure. The mice were fasted for 16 h, then intraperitoneally injected with 650 mg/kg APAP, and 10 h later, they were injected with ART (n = 10). (Q) Survival curve of mice treated with ART after the administration of a lethal dose of APAP (n = 10). Data are presented as mean ± SEM. ** P < 0.01.
Article Snippet: Artesunate (S24000;98%/ B20992 ) and Acetaminophen ( S31044 ;99%, AR/ B20338 ) were purchased from Shanghai Yuanye Bio‐Technology Co., Ltd. (Shanghai, China).
Techniques: Injection, TUNEL Assay, Staining