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actin related protein 2 3 complex subunit 1b  (Proteintech)


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    Structured Review

    Proteintech actin related protein 2 3 complex subunit 1b
    Actin Related Protein 2 3 Complex Subunit 1b, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/arpc1b/ARPC1B+Antibody/10__1096_slash_fj__202502867rr-146-17-40
    Average 93 stars, based on 12 article reviews
    actin related protein 2 3 complex subunit 1b - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer
    Article Snippet: .. After blocking endogenous peroxidase activity and tissue blocking, sections were incubated with primary antibodies against AP2M1 (1:200, #ET1612-33, Proteintech, Wuhan, China), TERF2IP (1:50, #14595-1-AP; Proteintech, Wuhan, China), ARPC1B (1:500; #28368-1-AP; Proteintech, Wuhan, China), LY6E (1:200; #ab300399; abcam, England), ARL4C (1:200; #10202-1-AP; Proteintech, Wuhan, China) at room temperature for 1 h. Subsequently, biotin-conjugated secondary reagents were applied for 45 min. ..

    Article Title: A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer.
    Article Snippet: .. After blocking endogenous peroxidase activity and tissue blocking, sections were incubated with primary antibodies against AP2M1 (1:200, #ET1612-33, Proteintech, Wuhan, China), TERF2IP (1:50, #14595- 1-AP; Proteintech, Wuhan, China), ARPC1B (1:500; #28368-1-AP; Proteintech, Wuhan, China), LY6E (1:200; #ab300399; abcam, England), ARL4C (1:200; #10202-1- AP; Proteintech, Wuhan, China) at room temperature for 1 h. Subsequently, biotin-conjugated secondary reagents were applied for 45 min. ..

    Activity Assay:

    Article Title: A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer
    Article Snippet: .. After blocking endogenous peroxidase activity and tissue blocking, sections were incubated with primary antibodies against AP2M1 (1:200, #ET1612-33, Proteintech, Wuhan, China), TERF2IP (1:50, #14595-1-AP; Proteintech, Wuhan, China), ARPC1B (1:500; #28368-1-AP; Proteintech, Wuhan, China), LY6E (1:200; #ab300399; abcam, England), ARL4C (1:200; #10202-1-AP; Proteintech, Wuhan, China) at room temperature for 1 h. Subsequently, biotin-conjugated secondary reagents were applied for 45 min. ..

    Article Title: A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer.
    Article Snippet: .. After blocking endogenous peroxidase activity and tissue blocking, sections were incubated with primary antibodies against AP2M1 (1:200, #ET1612-33, Proteintech, Wuhan, China), TERF2IP (1:50, #14595- 1-AP; Proteintech, Wuhan, China), ARPC1B (1:500; #28368-1-AP; Proteintech, Wuhan, China), LY6E (1:200; #ab300399; abcam, England), ARL4C (1:200; #10202-1- AP; Proteintech, Wuhan, China) at room temperature for 1 h. Subsequently, biotin-conjugated secondary reagents were applied for 45 min. ..

    Incubation:

    Article Title: A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer
    Article Snippet: .. After blocking endogenous peroxidase activity and tissue blocking, sections were incubated with primary antibodies against AP2M1 (1:200, #ET1612-33, Proteintech, Wuhan, China), TERF2IP (1:50, #14595-1-AP; Proteintech, Wuhan, China), ARPC1B (1:500; #28368-1-AP; Proteintech, Wuhan, China), LY6E (1:200; #ab300399; abcam, England), ARL4C (1:200; #10202-1-AP; Proteintech, Wuhan, China) at room temperature for 1 h. Subsequently, biotin-conjugated secondary reagents were applied for 45 min. ..

    Article Title: A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer.
    Article Snippet: .. After blocking endogenous peroxidase activity and tissue blocking, sections were incubated with primary antibodies against AP2M1 (1:200, #ET1612-33, Proteintech, Wuhan, China), TERF2IP (1:50, #14595- 1-AP; Proteintech, Wuhan, China), ARPC1B (1:500; #28368-1-AP; Proteintech, Wuhan, China), LY6E (1:200; #ab300399; abcam, England), ARL4C (1:200; #10202-1- AP; Proteintech, Wuhan, China) at room temperature for 1 h. Subsequently, biotin-conjugated secondary reagents were applied for 45 min. ..

    Electrophoresis:

    Article Title: Integrated Mendelian Randomization and Single-Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy.
    Article Snippet: The secondary antibodies used were Anti- Rabbit IgG (H + L) Secondary Antibody, HRP (#31460; 1:3000, Thermo Fisher), and Anti- Mouse IgG (H + L) Secondary Antibody, HRP (#13–4813- 85; 1:3000, Thermo Fisher). .. For protein capillary electrophoresis, the primary antibodies used were IQGAP1 (#ab133490, 1:50, Abcam), BKRB2 (#YN2508; 1:50, Immunoway), PAK2 (#2608S, 1:50, CST), ARPC1B (#28368- 1- AP, 1:50, Proteintech), and RAC2 (#10735- 1- AP; 1:50, Proteintech). .. The secondary antibodies used followed the manufacturer's instructions: Anti- Rabbit Secondary HRP Antibody (#042–206, 1:50, ProteinSimple), and Anti- Mouse Secondary HRP Antibody (#042–205, 1:50, ProteinSimple).

    Article Title: Integrated Mendelian Randomization and Single‐Cell Transcriptomics Analysis Identifies Critical Blood Biomarkers and Potential Mechanisms in Epilepsy
    Article Snippet: The secondary antibodies used were Anti‐Rabbit IgG (H + L) Secondary Antibody, HRP (#31460; 1:3000, Thermo Fisher), and Anti‐Mouse IgG (H + L) Secondary Antibody, HRP (#13–4813‐85; 1:3000, Thermo Fisher). .. For protein capillary electrophoresis, the primary antibodies used were IQGAP1 (#ab133490, 1:50, Abcam), BKRB2 (#YN2508; 1:50, Immunoway), PAK2 (#2608S, 1:50, CST), ARPC1B (#28368‐1‐AP, 1:50, Proteintech), and RAC2 (#10735‐1‐AP; 1:50, Proteintech). .. The secondary antibodies used followed the manufacturer's instructions: Anti‐Rabbit Secondary HRP Antibody (#042–206, 1:50, ProteinSimple), and Anti‐Mouse Secondary HRP Antibody (#042–205, 1:50, ProteinSimple).

    Western Blot:

    Article Title: ARPC1B Promotes Clear Cell Renal Cell Carcinoma Progression via the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Antibodies utilized in this research included: β-actin (Cat# 66009-1-Ig; WB 1:10,000), ARPC1B (Cat# 13835-1-AP; WB 1:5,000, IHC 1:1000), c-Myc (Cat# 10828-1-AP; WB 1:5,000, IHC 1:2000), Cyclin D1 (Cat# 60186-1-Ig; WB 1:10,000, IHC 1:3000), GSK3β (Cat# 22104-1-AP; WB 1:5,000, IHC 1:400), Phospho-GSK3β (Ser9) (Cat# 14850-1-AP; WB 1:5,000, IHC 1:600), β-catenin (Cat# 51067-2-AP; WB 1:10,000, IHC 1:200), Vimentin (Cat# 60330-1-Ig; WB 1:10,000, IHC 1:3000), N-cadherin (Cat# 22018-1-AP; WB 1:6000, IHC 1:1000), E-cadherin (Cat# 20874-1-AP; WB 1:6000, IHC 1:100), ZEB1 (Cat# 21544-1-AP; WB 1:2000, IHC 1:100), and Ki-67 (Cat# 27309-1-AP; IHC 1:2000) can from Proteintech. .. The Wnt/β-catenin agonist 3 (Cat# CM20349, 30 μmol/mL) was obtained from Proteintech (Wuhan, China, Cat# CM20349).

    Immunohistochemistry:

    Article Title: ARPC1B Promotes Clear Cell Renal Cell Carcinoma Progression via the Wnt/β-Catenin Signaling Pathway
    Article Snippet: .. Antibodies utilized in this research included: β-actin (Cat# 66009-1-Ig; WB 1:10,000), ARPC1B (Cat# 13835-1-AP; WB 1:5,000, IHC 1:1000), c-Myc (Cat# 10828-1-AP; WB 1:5,000, IHC 1:2000), Cyclin D1 (Cat# 60186-1-Ig; WB 1:10,000, IHC 1:3000), GSK3β (Cat# 22104-1-AP; WB 1:5,000, IHC 1:400), Phospho-GSK3β (Ser9) (Cat# 14850-1-AP; WB 1:5,000, IHC 1:600), β-catenin (Cat# 51067-2-AP; WB 1:10,000, IHC 1:200), Vimentin (Cat# 60330-1-Ig; WB 1:10,000, IHC 1:3000), N-cadherin (Cat# 22018-1-AP; WB 1:6000, IHC 1:1000), E-cadherin (Cat# 20874-1-AP; WB 1:6000, IHC 1:100), ZEB1 (Cat# 21544-1-AP; WB 1:2000, IHC 1:100), and Ki-67 (Cat# 27309-1-AP; IHC 1:2000) can from Proteintech. .. The Wnt/β-catenin agonist 3 (Cat# CM20349, 30 μmol/mL) was obtained from Proteintech (Wuhan, China, Cat# CM20349).



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