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antidsred  (TaKaRa)


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    Structured Review

    TaKaRa antidsred
    Antidsred, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antidsred/Living+Colors+mCherry+Monoclonal+Antibody/pmc12775026-360-23-30
    Average 97 stars, based on 255 article reviews
    antidsred - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: In vivo analysis of cardiomyocyte proliferation during trabeculation.
    Article Snippet: Cardiomyocyte proliferation is crucial for cardiac growth, patterning and regeneration; however, few studies have investigated the behavior of dividing cardiomyocytes in vivo.. Here, we use timelapse imaging of beating hearts in combination with the FUCCI system to monitor the behavior of proliferating cardiomyocytes in developing zebrafish.. Confirming in vitro observations, sarcomere disassembly, as well as changes in cell shape and volume, precede cardiomyocyte cytokinesis.

    Article Title: DNA damage modulates sleep drive in basal cnidarians with divergent chronotypes
    Article Snippet: .. After blocking, in N. vectensis , tissues were incubated with a primary antibody: rabbit anti-γH2AX (GTX127342, GeneTex, Irvine, CA, USA) 1:200 dilution and antiDsRED (Living Colors® mCherry Monoclonal mouse Antibody, Clontech, Cat# 632543) 1:200 dilution in blocking buffer for 72 h at 4 °C, while in C. andromeda , tissues were incubated with the rabbit anti-γH2AX 1:200 dilution. .. Then, for both species, samples were washed in PBS + 0.1% Triton 3 x 15 min. N. vectensis were incubated for 1 h with a goat polyclonal secondary anti-rabbit IgG H&L (Alexa Fluor® 646 nm, 2 mg/ml, Invitrogen, Waltham, MA, USA) and anti-mouse IgG H&L (Alexa Fluor® 488 nm, 2 mg/ml, Invitrogen) and with DAPI at 1:1000 dilution in blocking buffer at room temperature.

    Article Title: Ectopic expression of the cation-chloride cotransporter KCC2 in blood exosomes as a biomarker for functional rehabilitation.
    Article Snippet: Location of the blood collection Approximate volume Mandibular vein 100–200 μL Cardiac puncture 0.5–1.0 mL Frontiers in Molecular Neuroscience 04 frontiersin.org Bis-Tris plus, Invitrogen by Thermo Fisher Scientific) and transferred to a nitrocellulose membrane (GE Healthcare Life Science). .. Following the application of a blocking solution containing Tris-buffered saline, 0.1% Tween and 5% bovine serum albumin (BSA), the membranes were incubated overnight at 4°C with primary antibodies diluted in a blocking solution (Tris-buffered saline/ 0.1% tween/ 2.5% BSA): antiDsred (Clontech, Living Color Ds-Red Polyclonal Antibody, 632,496), anti-KCC2 [home-made antibody, (Ludwig et al., 2003)], and Tubuline-β3 (Biolegend, 802001). .. Horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Agilent Dako) was used as secondary antibodies, diluted in 5% bovine albumin at room temperature for 2 h. Bands were then detected using SuperSignal West Pico (Thermo Fisher Scientific, #34080) and analyzed with the G:Box imaging system (Syngene).

    Incubation:

    Article Title: In vivo analysis of cardiomyocyte proliferation during trabeculation.
    Article Snippet: Cardiomyocyte proliferation is crucial for cardiac growth, patterning and regeneration; however, few studies have investigated the behavior of dividing cardiomyocytes in vivo.. Here, we use timelapse imaging of beating hearts in combination with the FUCCI system to monitor the behavior of proliferating cardiomyocytes in developing zebrafish.. Confirming in vitro observations, sarcomere disassembly, as well as changes in cell shape and volume, precede cardiomyocyte cytokinesis.

    Article Title: DNA damage modulates sleep drive in basal cnidarians with divergent chronotypes
    Article Snippet: .. After blocking, in N. vectensis , tissues were incubated with a primary antibody: rabbit anti-γH2AX (GTX127342, GeneTex, Irvine, CA, USA) 1:200 dilution and antiDsRED (Living Colors® mCherry Monoclonal mouse Antibody, Clontech, Cat# 632543) 1:200 dilution in blocking buffer for 72 h at 4 °C, while in C. andromeda , tissues were incubated with the rabbit anti-γH2AX 1:200 dilution. .. Then, for both species, samples were washed in PBS + 0.1% Triton 3 x 15 min. N. vectensis were incubated for 1 h with a goat polyclonal secondary anti-rabbit IgG H&L (Alexa Fluor® 646 nm, 2 mg/ml, Invitrogen, Waltham, MA, USA) and anti-mouse IgG H&L (Alexa Fluor® 488 nm, 2 mg/ml, Invitrogen) and with DAPI at 1:1000 dilution in blocking buffer at room temperature.

    Article Title: A Requirement for Nuclear Factor- B in Developmental and Plasticity-Associated Synaptogenesis
    Article Snippet: .. DIV 16 neurons were fixed with 4% paraformaldehyde with 4% sucrose for 30 min, permeabilized with 0.2% Triton X-100 for 30 min, blocked for 1 h in 10% BSA, and incubated with primary antibodies: anti-GFP (Invitrogen; A11120, A11122; 1:500), antidsRED (Clontech; 632496; 1:600), anti-Bassoon (Nventa Biopharmaceuticals; VAM-PS003; 1:600), and anti-vesicular GABA transporter (VGAT) (Synaptic Systems; 151003; 1:1000). .. Alexa Fluor 488 and 568 secondary antibodies (Invitrogen; 1:1000) were incubated for 1 h at room temperature in 10% BSA and mounted with 0.1 M n-propyl gallate in 50% glycerol.

    Article Title: Ectopic expression of the cation-chloride cotransporter KCC2 in blood exosomes as a biomarker for functional rehabilitation.
    Article Snippet: Location of the blood collection Approximate volume Mandibular vein 100–200 μL Cardiac puncture 0.5–1.0 mL Frontiers in Molecular Neuroscience 04 frontiersin.org Bis-Tris plus, Invitrogen by Thermo Fisher Scientific) and transferred to a nitrocellulose membrane (GE Healthcare Life Science). .. Following the application of a blocking solution containing Tris-buffered saline, 0.1% Tween and 5% bovine serum albumin (BSA), the membranes were incubated overnight at 4°C with primary antibodies diluted in a blocking solution (Tris-buffered saline/ 0.1% tween/ 2.5% BSA): antiDsred (Clontech, Living Color Ds-Red Polyclonal Antibody, 632,496), anti-KCC2 [home-made antibody, (Ludwig et al., 2003)], and Tubuline-β3 (Biolegend, 802001). .. Horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Agilent Dako) was used as secondary antibodies, diluted in 5% bovine albumin at room temperature for 2 h. Bands were then detected using SuperSignal West Pico (Thermo Fisher Scientific, #34080) and analyzed with the G:Box imaging system (Syngene).

    other:

    Article Title: Loss of Action via Neurotensin-Leptin Receptor Neurons Disrupts Leptin and Ghrelin-Mediated Control of Energy Balance
    Article Snippet: dsRed/Tomato , AntidsRed , 632496, Clonetech , Rabbit, polyclonal , 1:1000 , AB_10013483.

    Saline:

    Article Title: Ectopic expression of the cation-chloride cotransporter KCC2 in blood exosomes as a biomarker for functional rehabilitation.
    Article Snippet: Location of the blood collection Approximate volume Mandibular vein 100–200 μL Cardiac puncture 0.5–1.0 mL Frontiers in Molecular Neuroscience 04 frontiersin.org Bis-Tris plus, Invitrogen by Thermo Fisher Scientific) and transferred to a nitrocellulose membrane (GE Healthcare Life Science). .. Following the application of a blocking solution containing Tris-buffered saline, 0.1% Tween and 5% bovine serum albumin (BSA), the membranes were incubated overnight at 4°C with primary antibodies diluted in a blocking solution (Tris-buffered saline/ 0.1% tween/ 2.5% BSA): antiDsred (Clontech, Living Color Ds-Red Polyclonal Antibody, 632,496), anti-KCC2 [home-made antibody, (Ludwig et al., 2003)], and Tubuline-β3 (Biolegend, 802001). .. Horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG (Agilent Dako) was used as secondary antibodies, diluted in 5% bovine albumin at room temperature for 2 h. Bands were then detected using SuperSignal West Pico (Thermo Fisher Scientific, #34080) and analyzed with the G:Box imaging system (Syngene).



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