Review




Structured Review

Proteintech anticxcl9
Anticxcl9, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anticxcl9/MIG+Antibody/pmc10861991__mmc2-345-23-25
Average 93 stars, based on 29 article reviews
anticxcl9 - by Bioz Stars, 2026-10
93/100 stars

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Related Articles

Blocking Assay:

Article Title: Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8 Tcell activity
Article Snippet: After measuring the total protein concentration using a BCA protein kit (P0009; Beyotime), the proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes. .. After blocking with 5% skimmed milk at room temperature for 2 h, the membrane was incubated with 1:1,000 dilution of anti-C5aR (21316-1-AP; Proteintech), antiCXCL9 (22355-1-AP; Proteintech), anti-p-Erk (28733-1-AP; Proteintech), anti-Erk (11257-1-AP; Proteintech), anti-p-Akt (66444-1-Ig; Proteintech), anti-Akt (60203-2-Ig; Proteintech), anti-p-NF-kB/p65 (3033T; Cell Signaling Technology, Danvers, MA, USA), anti-NFkB/p65 (80979-1-RR; Proteintech), anti-p-C/EBPb (AP1055; ABclonal, Wuhan, China), anti-C/EBPb (A19538; ABclonal), anti-pSTAT1 (AF330; ABclonal), anti-STAT1 (10144-2-AP; Proteintech), anti-p-STAT6 (AF3301; ABclonal), anti-STAT6 (51073-1-AP; Proteintech), or anti-b-actin (81115-1-RR; Proteintech) overnight. .. Thereafter it was washed and incubated with the corresponding secondary antibody (Proteintech) for 2 h. A chemiluminescent kit was used to detect the antibody-bound protein.

Article Title: Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8 + T cell activity.
Article Snippet: After measuring the total protein concentration using a BCA protein kit (P0009; Beyotime), the proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes. .. After blocking with 5% skimmed milk at room temperature for 2 h, the membrane was incubated with 1:1,000 dilution of anti-C5aR (21316-1-AP; Proteintech), antiCXCL9 (22355-1-AP; Proteintech), anti-p-Erk (28733-1-AP; Proteintech), anti-Erk (11257-1-AP; Proteintech), anti-p-Akt (66444-1-Ig; Proteintech), anti-Akt (60203-2-Ig; Proteintech), anti-p-NF-kB/p65 (3033T; Cell Signaling Technology, Danvers, MA, USA), anti-NFkB/p65 (80979-1-RR; Proteintech), anti-p-C/EBPb (AP1055; ABclonal, Wuhan, China), anti-C/EBPb (A19538; ABclonal), anti-pSTAT1 (AF330; ABclonal), anti-STAT1 (10144-2-AP; Proteintech), anti-p-STAT6 (AF3301; ABclonal), anti-STAT6 (51073-1-AP; Proteintech), or anti-b-actin (81115-1-RR; Proteintech) overnight. .. Thereafter it was washed and incubated with the corresponding secondary antibody (Proteintech) for 2 h. A chemiluminescent kit was used to detect the antibody-bound protein.

Membrane:

Article Title: Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8 Tcell activity
Article Snippet: After measuring the total protein concentration using a BCA protein kit (P0009; Beyotime), the proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes. .. After blocking with 5% skimmed milk at room temperature for 2 h, the membrane was incubated with 1:1,000 dilution of anti-C5aR (21316-1-AP; Proteintech), antiCXCL9 (22355-1-AP; Proteintech), anti-p-Erk (28733-1-AP; Proteintech), anti-Erk (11257-1-AP; Proteintech), anti-p-Akt (66444-1-Ig; Proteintech), anti-Akt (60203-2-Ig; Proteintech), anti-p-NF-kB/p65 (3033T; Cell Signaling Technology, Danvers, MA, USA), anti-NFkB/p65 (80979-1-RR; Proteintech), anti-p-C/EBPb (AP1055; ABclonal, Wuhan, China), anti-C/EBPb (A19538; ABclonal), anti-pSTAT1 (AF330; ABclonal), anti-STAT1 (10144-2-AP; Proteintech), anti-p-STAT6 (AF3301; ABclonal), anti-STAT6 (51073-1-AP; Proteintech), or anti-b-actin (81115-1-RR; Proteintech) overnight. .. Thereafter it was washed and incubated with the corresponding secondary antibody (Proteintech) for 2 h. A chemiluminescent kit was used to detect the antibody-bound protein.

Article Title: Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8 + T cell activity.
Article Snippet: After measuring the total protein concentration using a BCA protein kit (P0009; Beyotime), the proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes. .. After blocking with 5% skimmed milk at room temperature for 2 h, the membrane was incubated with 1:1,000 dilution of anti-C5aR (21316-1-AP; Proteintech), antiCXCL9 (22355-1-AP; Proteintech), anti-p-Erk (28733-1-AP; Proteintech), anti-Erk (11257-1-AP; Proteintech), anti-p-Akt (66444-1-Ig; Proteintech), anti-Akt (60203-2-Ig; Proteintech), anti-p-NF-kB/p65 (3033T; Cell Signaling Technology, Danvers, MA, USA), anti-NFkB/p65 (80979-1-RR; Proteintech), anti-p-C/EBPb (AP1055; ABclonal, Wuhan, China), anti-C/EBPb (A19538; ABclonal), anti-pSTAT1 (AF330; ABclonal), anti-STAT1 (10144-2-AP; Proteintech), anti-p-STAT6 (AF3301; ABclonal), anti-STAT6 (51073-1-AP; Proteintech), or anti-b-actin (81115-1-RR; Proteintech) overnight. .. Thereafter it was washed and incubated with the corresponding secondary antibody (Proteintech) for 2 h. A chemiluminescent kit was used to detect the antibody-bound protein.

Incubation:

Article Title: Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8 Tcell activity
Article Snippet: After measuring the total protein concentration using a BCA protein kit (P0009; Beyotime), the proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes. .. After blocking with 5% skimmed milk at room temperature for 2 h, the membrane was incubated with 1:1,000 dilution of anti-C5aR (21316-1-AP; Proteintech), antiCXCL9 (22355-1-AP; Proteintech), anti-p-Erk (28733-1-AP; Proteintech), anti-Erk (11257-1-AP; Proteintech), anti-p-Akt (66444-1-Ig; Proteintech), anti-Akt (60203-2-Ig; Proteintech), anti-p-NF-kB/p65 (3033T; Cell Signaling Technology, Danvers, MA, USA), anti-NFkB/p65 (80979-1-RR; Proteintech), anti-p-C/EBPb (AP1055; ABclonal, Wuhan, China), anti-C/EBPb (A19538; ABclonal), anti-pSTAT1 (AF330; ABclonal), anti-STAT1 (10144-2-AP; Proteintech), anti-p-STAT6 (AF3301; ABclonal), anti-STAT6 (51073-1-AP; Proteintech), or anti-b-actin (81115-1-RR; Proteintech) overnight. .. Thereafter it was washed and incubated with the corresponding secondary antibody (Proteintech) for 2 h. A chemiluminescent kit was used to detect the antibody-bound protein.

Article Title: Blockade of C5a receptor unleashes tumor-associated macrophage antitumor response and enhances CXCL9-dependent CD8 + T cell activity.
Article Snippet: After measuring the total protein concentration using a BCA protein kit (P0009; Beyotime), the proteins were separated by electrophoresis and transferred to polyvinylidene fluoride membranes. .. After blocking with 5% skimmed milk at room temperature for 2 h, the membrane was incubated with 1:1,000 dilution of anti-C5aR (21316-1-AP; Proteintech), antiCXCL9 (22355-1-AP; Proteintech), anti-p-Erk (28733-1-AP; Proteintech), anti-Erk (11257-1-AP; Proteintech), anti-p-Akt (66444-1-Ig; Proteintech), anti-Akt (60203-2-Ig; Proteintech), anti-p-NF-kB/p65 (3033T; Cell Signaling Technology, Danvers, MA, USA), anti-NFkB/p65 (80979-1-RR; Proteintech), anti-p-C/EBPb (AP1055; ABclonal, Wuhan, China), anti-C/EBPb (A19538; ABclonal), anti-pSTAT1 (AF330; ABclonal), anti-STAT1 (10144-2-AP; Proteintech), anti-p-STAT6 (AF3301; ABclonal), anti-STAT6 (51073-1-AP; Proteintech), or anti-b-actin (81115-1-RR; Proteintech) overnight. .. Thereafter it was washed and incubated with the corresponding secondary antibody (Proteintech) for 2 h. A chemiluminescent kit was used to detect the antibody-bound protein.



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