anti-map2 antibody  (Millipore)

Journal: bioRxiv

Article Title: The biochemical nature of senile plaques: intracellular destabilized-lysosome-enriched neural processes catabolizing extracellular hemorrhagic amyloid materials

doi: 10.64898/2025.12.30.696965

Figure Lengend Snippet: Eight representative images were shown. Quantitation of the intensity of MAP2 in senile plaques vs. regions outside of senile plaques showed that senile plaque region has greatly reduced MAP2 intensity, which is around 0.69±0.08 (N=8) comparing to the immediate surrounding region MAP2 levels. The yellow arrows indicated the beading-type MAP2 staining of the degenerative neuronal processes. The white arrows indicated the weak diffusive type of MAP2 staining in degenerating neurites. The white dashed circles indicated senile plaques. The yellow dashed lines indicate several relatively long neurites with both Aβ deposition and beading-type degenerative MAP2 staining. The blue arrows indicated granule-type Aβ staining in the senile plaques. Scale bar, 25 µm.

Article Snippet: The following primary antibodies and dilutions have been used in this study: Aβ (Abcam ab201061, 1:200, Cambridge, UK), Aβ/AβPP (CST #2450, 1:200, Danvers, MA, USA), phos-Tau (Abcam ab151559, 1:200), MAP2 (Proteintech 17490-1-AP, 1:200, Rosemont, IL, USA), Sortilin1 (Abcam ab263864, 1:200), HBA (Abcam ab92492, 1:200), Cathepsin D (Abcam ab75852, 1:200), Lamp2 (Abcam ab199946, 1:200), Cathepsin D (Proteintech 66534-1-Ig, 1:100), Ceruloplasmin (Proteintech, 21131-1-AP, 1:200), Hemin (Absolute Antibody, 1D3, 1: 100, Redcar, UK), ApoE (Abcam ab183597, 1: 200), HbA1c (OkayBio K5a2, 1: 100, Nanjing, Jiangsu, China).

Techniques: Quantitation Assay, Staining

Journal: bioRxiv

Article Title: The biochemical nature of senile plaques: intracellular destabilized-lysosome-enriched neural processes catabolizing extracellular hemorrhagic amyloid materials

doi: 10.64898/2025.12.30.696965

Figure Lengend Snippet: Five samples were shown. Aβ was stained with green fluorescence while MAP2 was stained with red fluorescence. Microaneurysms were clearly identified with Phase Contrast microscopy. The average Aβ staining intensity in microaneurysms is around 3.03±0.97 (N=5) times of the average Aβ intensity of the immediate tissue environment. White dashed lines indicated the regions of microaneurysms. White arrows point to neighboring neurons with MAP2 immunostaining. Yellow dashed lines indicated two long neurites showing beading type degenerative MAP2 staining. Scale bar, 50 µm.

Article Snippet: The following primary antibodies and dilutions have been used in this study: Aβ (Abcam ab201061, 1:200, Cambridge, UK), Aβ/AβPP (CST #2450, 1:200, Danvers, MA, USA), phos-Tau (Abcam ab151559, 1:200), MAP2 (Proteintech 17490-1-AP, 1:200, Rosemont, IL, USA), Sortilin1 (Abcam ab263864, 1:200), HBA (Abcam ab92492, 1:200), Cathepsin D (Abcam ab75852, 1:200), Lamp2 (Abcam ab199946, 1:200), Cathepsin D (Proteintech 66534-1-Ig, 1:100), Ceruloplasmin (Proteintech, 21131-1-AP, 1:200), Hemin (Absolute Antibody, 1D3, 1: 100, Redcar, UK), ApoE (Abcam ab183597, 1: 200), HbA1c (OkayBio K5a2, 1: 100, Nanjing, Jiangsu, China).

Techniques: Staining, Fluorescence, Microscopy, Immunostaining