lamp2  (Proteintech)

Journal: bioRxiv

Article Title: Heparan sulfate promotes autoactivation of pro-Cathepsin K by destabilizing the propeptide-catalytic domain interaction

doi: 10.64898/2026.01.18.700217

Figure Lengend Snippet: Mouse femur section was co-stained with anti-CtsK (green), HS20 (purple) and anti-LAMP2 (red) and visualized with anti-rabbit Alexa488, anti-human Alexa594, and anti-rat Alexa647 secondary antibodies, respectively. Area shown is the cortical bone. The approximate boundary of the bone is shown with white dashed line. A blood vessel within the bone is indicated with yellow dashed line. Red blood cells in the vessel shown strong green autofluorescence. A closeup view of the large osteoclast is shown in the right bottom panel. Another osteoclast (likely only the tip of the osteoclast) is indicated with a yellow arrow in the merged image. Nuclei of several osteocytes are indicated with white arrow head. Images representative of three separate experiments.

Article Snippet: Following deparaffinization and citric acid-based antigen retrieval (pH7), sectioned were blocked and incubated overnight at 4 °C with the following primary antibodies: 0.2 μg/ml rabbit anti-mCtsK polyclonal antibody (described previously) , 1ug/ml human anti-HS mAb (HS20, from Bio X cell) , and 1ug/ml Rat anti-mouse Lamp2 (GL2A7, Developmental Studies Hybridoma Bank).

Techniques: Staining

Journal: Acta Neuropathologica Communications

Article Title: Pathobiology of the autophagy-lysosomal pathway in the Huntington’s disease brain

doi: 10.1186/s40478-025-02131-8

Figure Lengend Snippet: Correlation between marker expression levels and HD progression stages across different brain regions, grouped by marker category. The heatmap illustrates Spearman’s rank correlation coefficients (ρ) between the relative mRNA expression levels from qPCR of selected molecular markers and Huntington’s disease (HD) severity. Analyses were performed on human post-mortem brain tissue from control (Ctl) and HD patients (grades HD2, HD3, HD4), with severity numerically encoded for correlation (Ctl = 0, HD2 = 1, HD3 = 2, HD4 = 3). Correlations are shown for STR, CTX, and CBM). Markers are grouped into functional categories, displayed from top to bottom: Induction Markers (Autophagy induction): ATG7, LC3, p62. Lysosomal Markers (Lysosomal biogenesis, hydrolases and structural elements): TFEB, TFE3, CTSB, CTSD, HEXA, LAMP1, LAMP2. Cellular Markers (cellular processes): ENO2, TUBB3, GFAP, AIF1, MBP. The color of each cell represents the Spearman ρ value, with a continuous gradient from blue (strong negative correlation, ρ = −1) through white (no correlation, ρ = 0) to red (strong positive correlation, ρ = + 1). Nominal p-values for the correlations are indicated by asterisks: * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Antibodies for immunohistochemistry (IHC), western blotting (WB): The following primary antibodies were used in this study. (1) from Cell Signaling Technology: tHTT rabbit mAb (clone D7F7, #5656, targeting residues surrounding Pro1220 of human HTT and detecting total HTTs), p70S6K pAb (#9202), p-p70S6K (T389) pAb (#9205), ULK1 pAb (#4773), p-ULK1 (S757) pAb (#6888, #14202; detecting S757 or S758 of mouse or human ULK1, respectively), ATG5 rabbit mAb (#12994), ATG7 pAb (#2631), ATG13 rabbit mAb (#13273), p-ATG13 (S355) rabbit mAb (#26839), VPS34 rabbit mAb (#81453), TRAF6 rabbit mAb (#8028), Calnexin rabbit mAb (#2679). (2) from Millipore-Sigma: ntHTT mAb (N-Terminus-specific, mEM48, #MAB5374, preferentially recognizing aggregated HTT) , ATG5 pAb (#ABC14), K48- or K63-specific ubiquitin mAb (#05–1307, #05–1308, respectively), βIII-tubulin mAb (#SAB4700544), β–actin mAb (#A1978). (3) from other vendors: BECN1 mAb (BD Biosciences, #612113); LC3 pAb (Novus Biologics, #NB100-2220), ATG9 (Novus Biologics, #B-110–56893); p62 mAb (BD Biosciences, #610832) or C-term-specific p62 Guinea Pig pAb (Progen Biotechnik, #C-1620); total ubiquitin pAb (Dako Agilent, #Z0458), LAMP1 or LAMP2 rat mAb (Developmental Studies Hybridoma Bank, University of Iowa, #H4A3 or #H4B4, respectively); CTSD sheep pAb (D-2–3, in-house made) ( ); CTSD pAb (Scripps Laboratories, #RC245), CTSD mAb (CD1.1, in-house made) ( ); CTSB pAb (Cortex Biochemicals, #CR6009RP), CTSB goat pAb (Neuromics, #GT15047).

Techniques: Marker, Expressing, Control, Functional Assay