Recombinant:Article Title: CellBouncer, a unified toolkit for single-cell demultiplexing and ambient RNA analysis, reveals hominid mitochondrial incompatibilities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning DMEM/F12 With L-Glutamine and 15 mM HEPES ThermoFisher Cat# MT10092CM MEM alpha, nucleosides, GlutaMAX Supplement (α-MEM) ThermoFisher Cat# 32571-036 Corning Fetal Bovine Serum (FBS) ThermoFisher Cat# 35015CV MEM Non-Essential Amino Acids Solution (100×) ThermoFisher Cat# 11140050 Glutamax I Supplement ThermoFisher Cat# 15140122 DPBS, no calcium, no magnesium ThermoFisher Cat# 14190250 Accutase ThermoFisher Cat# AT104-500 Primocin Invivogen Cat# ant-pm-2 Puromycin ThermoFisher Cat# A1113803 Zeocin ThermoFisher Cat# J67140-8EQ Insulin Gendepot Cat# I4600-010 Holo-Transferrin Invitria Cat# 777TRF029 Recombinant human TGFβ1 Peprotech Cat# 100-21C Recombinant human NRG1 Peprotech Cat# 100-03 FGF2-G3 (RPPC), Northwestern University Derived from Addgene: #135521 Bovine Serum Albumin (BSA) Gendepot Cat# A0100-100 Vitronectin (VTN-N) Recombinant Human Protein ThermoFisher Cat# A14700 Corning Growth Factor Reduced Matrigel ThermoFisher Cat# CB40234B L-ascorbic acid 2-phosphate trisodium salt Fujifilm Cat# 011-2847 Heparin Sigma Cat# H3393 Sodium Selenite Sigma Cat# S5261 Poly(Vinyl Alcohol), 87–90% hydrolyzed Sigma Cat# P8136 Adenosine Sigma Cat# A4036 Guanosine Sigma Cat# G6264 Cytidine Sigma Cat# C4654 Uridine Sigma Cat# U3003 Thymidine Sigma Cat# T1895 Sodium butyrate Selleck Chemicals Cat# S1999 Hydrocortisone Sigma Aldrich Cat# H0888 Dorsomorphin Selleck Chemicals Cat# S7306 Thiazovivin MedChemExpress Cat# HY-13257 IWR1-Endo AxonMedchem Cat# 2510 Emricassan (CEPT component) MedKoo Cat# 510230 Trans-ISRIB (CEPT component) MedChemExpress Cat# HY-12495 Chroman-1 (CEPT component) MedChemExpress Cat# HY-15392 Spermine (CEPT component) Sigma Aldrich Cat# S4264 Putrescine dihydrochloride (CEPT component) Sigma Aldrich Cat# P5780 Spermidine (CEPT component) Sigma Aldrich Cat# S0266 Roche PEG 1500 Sigma Aldrich Cat# 10783641001 (Continued on next page) e1 Cell Genomics 6, 101275, July 8, 2026 Resource ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Corning DMSO ThermoFisher Cat# 25-950-CQC Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#1000268 Deposited data Composite iPSC single-cell RNA-seq reads This study BioProject: PRJNA1358605 Whole genome sequencing data from cell lines This study BioProject: PRJNA1358606 Single-cell gene-expression matrices from composite iPSCs This study GEO: GSE329917 Human/chimpanzee/bonobo ancestor genome and annotation This study, with data from Mao73 https://doi.org/10.6084/m9.figshare.
Article Title: Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Mix, *LDEV-free Corning Cat# 354230 OPTI-MEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# 11965092 Fetal Bovine Serum (FBS) Sigma Aldrich Cat# F2442 Trypsin-EDTA (0.05%) Thermo Fisher Cat# 25300054 Polybrene Sigma Aldrich Cat# TR-1003 TRIzol Reagent Thermo Fisher Cat# 15596018 M-PER Extraction Reagent Thermo Fisher Cat# 78501 Advanced DMEM/F12 Thermo Fisher Cat# 12634010 N2 Supplement Thermo Fisher Cat# 17502048 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat# 12587010 Primocin Invivogen Cat# ant-pm-1 N-Acetyl-L-cysteine Sigma Aldrich Cat# A9165 L-glutamine Thermo Fisher Cat# 25030024 Nicotinamide Sigma Aldrich Cat# N0636 ALK-4,-5,-7 inhibitor, A83-01 Tocris Cat# 2939 Recombinant human EGF Peprotech Cat# AF-100-15 (Continued on next page) 2 STAR Protocols 7, 104558, June 19, 2026 Note: If human Noggin, Wnt3a, and R-spondin conditioned media are unavailable, you can instead directly add recombinant human Noggin protein (100 ng/mL; R&D Systems Cat#6057-NG), Wnt3a protein (200 ng/mL; R&D Systems Cat#5036-WN), and R-Spondin 1 protein (200 ng/mL; R&D Systems Cat#4645-RS). .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Mix, *LDEV-free Corning Cat# 354230 OPTI-MEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# 11965092 Fetal Bovine Serum (FBS) Sigma Aldrich Cat# F2442 Trypsin-EDTA (0.05%) Thermo Fisher Cat# 25300054 Polybrene Sigma Aldrich Cat# TR-1003 TRIzol Reagent Thermo Fisher Cat# 15596018 M-PER Extraction Reagent Thermo Fisher Cat# 78501 Advanced DMEM/F12 Thermo Fisher Cat# 12634010 N2 Supplement Thermo Fisher Cat# 17502048 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat# 12587010 Primocin Invivogen Cat# ant-pm-1 N-Acetyl-L-cysteine Sigma Aldrich Cat# A9165 L-glutamine Thermo Fisher Cat# 25030024 Nicotinamide Sigma Aldrich Cat# N0636 ALK-4,-5,-7 inhibitor, A83-01 Tocris Cat# 2939 Recombinant human EGF Peprotech Cat# AF-100-15 (Continued on next page) 2 STAR Protocols 7, 104558, June 19, 2026 Note: If human Noggin, Wnt3a, and R-spondin conditioned media are unavailable, you can instead directly add recombinant human Noggin protein (100 ng/mL; R&D Systems Cat#6057-NG), Wnt3a protein (200 ng/mL; R&D Systems Cat#5036-WN), and R-Spondin 1 protein (200 ng/mL; R&D Systems Cat#4645-RS).
Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Derivative Assay:Article Title: CellBouncer, a unified toolkit for single-cell demultiplexing and ambient RNA analysis, reveals hominid mitochondrial incompatibilities.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning DMEM/F12 With L-Glutamine and 15 mM HEPES ThermoFisher Cat# MT10092CM MEM alpha, nucleosides, GlutaMAX Supplement (α-MEM) ThermoFisher Cat# 32571-036 Corning Fetal Bovine Serum (FBS) ThermoFisher Cat# 35015CV MEM Non-Essential Amino Acids Solution (100×) ThermoFisher Cat# 11140050 Glutamax I Supplement ThermoFisher Cat# 15140122 DPBS, no calcium, no magnesium ThermoFisher Cat# 14190250 Accutase ThermoFisher Cat# AT104-500 Primocin Invivogen Cat# ant-pm-2 Puromycin ThermoFisher Cat# A1113803 Zeocin ThermoFisher Cat# J67140-8EQ Insulin Gendepot Cat# I4600-010 Holo-Transferrin Invitria Cat# 777TRF029 Recombinant human TGFβ1 Peprotech Cat# 100-21C Recombinant human NRG1 Peprotech Cat# 100-03 FGF2-G3 (RPPC), Northwestern University Derived from Addgene: #135521 Bovine Serum Albumin (BSA) Gendepot Cat# A0100-100 Vitronectin (VTN-N) Recombinant Human Protein ThermoFisher Cat# A14700 Corning Growth Factor Reduced Matrigel ThermoFisher Cat# CB40234B L-ascorbic acid 2-phosphate trisodium salt Fujifilm Cat# 011-2847 Heparin Sigma Cat# H3393 Sodium Selenite Sigma Cat# S5261 Poly(Vinyl Alcohol), 87–90% hydrolyzed Sigma Cat# P8136 Adenosine Sigma Cat# A4036 Guanosine Sigma Cat# G6264 Cytidine Sigma Cat# C4654 Uridine Sigma Cat# U3003 Thymidine Sigma Cat# T1895 Sodium butyrate Selleck Chemicals Cat# S1999 Hydrocortisone Sigma Aldrich Cat# H0888 Dorsomorphin Selleck Chemicals Cat# S7306 Thiazovivin MedChemExpress Cat# HY-13257 IWR1-Endo AxonMedchem Cat# 2510 Emricassan (CEPT component) MedKoo Cat# 510230 Trans-ISRIB (CEPT component) MedChemExpress Cat# HY-12495 Chroman-1 (CEPT component) MedChemExpress Cat# HY-15392 Spermine (CEPT component) Sigma Aldrich Cat# S4264 Putrescine dihydrochloride (CEPT component) Sigma Aldrich Cat# P5780 Spermidine (CEPT component) Sigma Aldrich Cat# S0266 Roche PEG 1500 Sigma Aldrich Cat# 10783641001 (Continued on next page) e1 Cell Genomics 6, 101275, July 8, 2026 Resource ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Corning DMSO ThermoFisher Cat# 25-950-CQC Critical commercial assays Chromium Next GEM Single Cell 3ʹ Kit v3.1 10× Genomics Cat#1000268 Deposited data Composite iPSC single-cell RNA-seq reads This study BioProject: PRJNA1358605 Whole genome sequencing data from cell lines This study BioProject: PRJNA1358606 Single-cell gene-expression matrices from composite iPSCs This study GEO: GSE329917 Human/chimpanzee/bonobo ancestor genome and annotation This study, with data from Mao73 https://doi.org/10.6084/m9.figshare.
other:Article Title: Human models of GLE1-associated LCCS1 reveal neural crest deficiency and multisystem developmental failure accompanied by altered RNA metabolism
Article Snippet: MN differentiation base media (MNb) containing DMEM/F12 (11320033, Thermo Fisher), Neurobasal medium (21103049, Thermo Fisher) 1:1, 0.5× N2 supplement (17502048, Thermo fisher), 0.5× B27 (17504044, Thermo Fisher), L-ascorbic acid 0.1 mM (CAS 50-81-7, Santa Cruz) and Primocin 100 μg/ml (ant-pm-05, Invivogen) were used throughout the differentiation.
Membrane:Article Title: Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Mix, *LDEV-free Corning Cat# 354230 OPTI-MEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# 11965092 Fetal Bovine Serum (FBS) Sigma Aldrich Cat# F2442 Trypsin-EDTA (0.05%) Thermo Fisher Cat# 25300054 Polybrene Sigma Aldrich Cat# TR-1003 TRIzol Reagent Thermo Fisher Cat# 15596018 M-PER Extraction Reagent Thermo Fisher Cat# 78501 Advanced DMEM/F12 Thermo Fisher Cat# 12634010 N2 Supplement Thermo Fisher Cat# 17502048 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat# 12587010 Primocin Invivogen Cat# ant-pm-1 N-Acetyl-L-cysteine Sigma Aldrich Cat# A9165 L-glutamine Thermo Fisher Cat# 25030024 Nicotinamide Sigma Aldrich Cat# N0636 ALK-4,-5,-7 inhibitor, A83-01 Tocris Cat# 2939 Recombinant human EGF Peprotech Cat# AF-100-15 (Continued on next page) 2 STAR Protocols 7, 104558, June 19, 2026 Note: If human Noggin, Wnt3a, and R-spondin conditioned media are unavailable, you can instead directly add recombinant human Noggin protein (100 ng/mL; R&D Systems Cat#6057-NG), Wnt3a protein (200 ng/mL; R&D Systems Cat#5036-WN), and R-Spondin 1 protein (200 ng/mL; R&D Systems Cat#4645-RS). .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Mix, *LDEV-free Corning Cat# 354230 OPTI-MEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# 11965092 Fetal Bovine Serum (FBS) Sigma Aldrich Cat# F2442 Trypsin-EDTA (0.05%) Thermo Fisher Cat# 25300054 Polybrene Sigma Aldrich Cat# TR-1003 TRIzol Reagent Thermo Fisher Cat# 15596018 M-PER Extraction Reagent Thermo Fisher Cat# 78501 Advanced DMEM/F12 Thermo Fisher Cat# 12634010 N2 Supplement Thermo Fisher Cat# 17502048 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat# 12587010 Primocin Invivogen Cat# ant-pm-1 N-Acetyl-L-cysteine Sigma Aldrich Cat# A9165 L-glutamine Thermo Fisher Cat# 25030024 Nicotinamide Sigma Aldrich Cat# N0636 ALK-4,-5,-7 inhibitor, A83-01 Tocris Cat# 2939 Recombinant human EGF Peprotech Cat# AF-100-15 (Continued on next page) 2 STAR Protocols 7, 104558, June 19, 2026 Note: If human Noggin, Wnt3a, and R-spondin conditioned media are unavailable, you can instead directly add recombinant human Noggin protein (100 ng/mL; R&D Systems Cat#6057-NG), Wnt3a protein (200 ng/mL; R&D Systems Cat#5036-WN), and R-Spondin 1 protein (200 ng/mL; R&D Systems Cat#4645-RS).
Extraction:Article Title: Protocol for lentiviral generation and transduction of human-derived endometrial epithelial organoids to study uterine function and pathologies.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Mix, *LDEV-free Corning Cat# 354230 OPTI-MEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# 11965092 Fetal Bovine Serum (FBS) Sigma Aldrich Cat# F2442 Trypsin-EDTA (0.05%) Thermo Fisher Cat# 25300054 Polybrene Sigma Aldrich Cat# TR-1003 TRIzol Reagent Thermo Fisher Cat# 15596018 M-PER Extraction Reagent Thermo Fisher Cat# 78501 Advanced DMEM/F12 Thermo Fisher Cat# 12634010 N2 Supplement Thermo Fisher Cat# 17502048 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat# 12587010 Primocin Invivogen Cat# ant-pm-1 N-Acetyl-L-cysteine Sigma Aldrich Cat# A9165 L-glutamine Thermo Fisher Cat# 25030024 Nicotinamide Sigma Aldrich Cat# N0636 ALK-4,-5,-7 inhibitor, A83-01 Tocris Cat# 2939 Recombinant human EGF Peprotech Cat# AF-100-15 (Continued on next page) 2 STAR Protocols 7, 104558, June 19, 2026 Note: If human Noggin, Wnt3a, and R-spondin conditioned media are unavailable, you can instead directly add recombinant human Noggin protein (100 ng/mL; R&D Systems Cat#6057-NG), Wnt3a protein (200 ng/mL; R&D Systems Cat#5036-WN), and R-Spondin 1 protein (200 ng/mL; R&D Systems Cat#4645-RS). .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Mix, *LDEV-free Corning Cat# 354230 OPTI-MEM Thermo Fisher Cat# 31985070 DMEM Thermo Fisher Cat# 11965092 Fetal Bovine Serum (FBS) Sigma Aldrich Cat# F2442 Trypsin-EDTA (0.05%) Thermo Fisher Cat# 25300054 Polybrene Sigma Aldrich Cat# TR-1003 TRIzol Reagent Thermo Fisher Cat# 15596018 M-PER Extraction Reagent Thermo Fisher Cat# 78501 Advanced DMEM/F12 Thermo Fisher Cat# 12634010 N2 Supplement Thermo Fisher Cat# 17502048 B-27 Supplement (50X), minus vitamin A Thermo Fisher Cat# 12587010 Primocin Invivogen Cat# ant-pm-1 N-Acetyl-L-cysteine Sigma Aldrich Cat# A9165 L-glutamine Thermo Fisher Cat# 25030024 Nicotinamide Sigma Aldrich Cat# N0636 ALK-4,-5,-7 inhibitor, A83-01 Tocris Cat# 2939 Recombinant human EGF Peprotech Cat# AF-100-15 (Continued on next page) 2 STAR Protocols 7, 104558, June 19, 2026 Note: If human Noggin, Wnt3a, and R-spondin conditioned media are unavailable, you can instead directly add recombinant human Noggin protein (100 ng/mL; R&D Systems Cat#6057-NG), Wnt3a protein (200 ng/mL; R&D Systems Cat#5036-WN), and R-Spondin 1 protein (200 ng/mL; R&D Systems Cat#4645-RS).
Virus:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Modification:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Produced:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
Blocking Assay:Article Title: A dual-adjuvanted HA stem nanoparticle vaccine elicits a multifunctional antibody response that is associated with protection in newborn monkeys.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FI6v3 NIH Vaccine Research Center 75 N/A Sheep anti-mouse IgG-HRP Cytiva Cat# GENA931V Goat anti-monkey IgM-HRP Fitzgerald Cat# 43C-CB1603; RRID:AB_1287155 Goat anti-monkey IgA-Biotin Fitzgerald Cat# 43R-IG002bt; RRID:AB_1286557 NeutrAvidin-HRP Thermo Fisher Scientific Cat# 31001 PE anti-humanCD107a (LAMP-1) Biolegend Cat# 328608; Clone H4A3; RRID:AB_1186040 FITC IgG Goat Anti-Guinea Pig Complement C3 MP Biomedicals Cat# 855385 Bacterial and virus strains R3ΔPB1 H1N1 subtypes (A/California/07/09 & A/New Caledonia/20/99) NIH Vaccine Research Center 59 N/A R3ΔPB1 (R4ΔPB1) virus for H5N1 (A/Vietnam/1203/04) NIH Vaccine Research Center 59 N/A Chemicals, peptides, and recombinant proteins H1ssF S111C NIH Vaccine Research Center N/A SM(PEG) 4 Thermo Fisher Scientific Cat# 22104 Amine derivative of R848 that was modified to contain a primary amine Produced in house by Dr. B King 31 N/A A/NewCaledonia/20/1999 (H1N1) stabilized HA stem protein NIH Vaccine Research Center 24 N/A Casein blocking buffer 10X Sigma-Aldrich Cat# B6429 Goat serum Lampire Biologicals Cat# 50-413-254 3,3 ′ ,5,5 ′ -Tetramethylbenzidine dihydrochloride Sigma-Aldrich Cat# T3405-100TAB H1ssF-100XR-S Produced in house ‘‘this paper’’ N/A H1ssF-300XR-S Produced in house ‘‘this paper’’ N/A H1ssF-900XR-S Produced in house ‘‘this paper’’ N/A A/New Caledonia/20/1999 (H1) BEI Resources Cat# NR-48873 A/California/07/2009 (H1) BEI Resources Cat# NR-42635 A/Singapore/1/1957 (H2) BEI Resources Cat# NR-52249 A/Wisconsin/67/2005 (H3) BEI Resources Cat# NR-49237 A/Vietnam/1204/2004 (H5) NIH Vaccine Research Center N/A A/Anhui/1/2013 (H7) BEI Resources Cat# NR-44365 A/HongKong/33982/2009 (H9) BEI Resources Cat# NR-41792 H1-N27 NIH Vaccine Research Center 51 N/A H1-N45 NIH Vaccine Research Center 50 N/A H1-N45/27 NIH Vaccine Research Center 51 N/A NaSCN Acros Organics Cat# 196762500 AddaVax InvivoGen Cat# Vac-adx-10 Gill’s Hematoxylin II Poly Scientific R&D Corp. Cat# S210-1GL GAL Permount Mounting Medium Thermo Fisher Scientific Cat# SP15-500 Bovine serum albumin Sigma Cat# A9418-50G RPMI 1640 Gibco Thermo Fisher Scientific Cat# 11875093 Fetal Bovine Serum Atlanta Biologicals Cat# S11550 L-Glutamine Gibco Thermo Fisher Scientific Cat# 25030081 Primocin InvivoGen Cat# ant-pm-1 Recombinant human IL-2 Biolegend Cat# 589104 (Continued on next page) e1 Cell Reports Medicine 7, 102746, May 19, 2026 OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Biotinylated HA A/California/07/2009 DCVC, Duke University N/A FluoSpheres NeutrAvidin (yellow-green fluorescent (505/515)) Thermo Fisher Scientific Cat# F8776 FluoSpheres NeutrAvidin (non-fluorescent) Thermo Fisher Scientific Cat# F8777 Guinea pig complement Cedarlane Cat# CL4051 Veronal buffer with 0.1% gelatin GVB++.
|
InvivoGen is a verified supplier 
InvivoGen manufactures this product 