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appendix  (Proteintech)


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    Proteintech appendix
    Appendix, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/appendix/product/Proteintech
    Average 93 stars, based on 7 article reviews
    appendix - by Bioz Stars, 2026-03
    93/100 stars

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    Anillin promotes epithelial-mesenchymal transition (EMT), proliferation, migration, invasion, and angiogenesis in BxPC-3 cells. (A) Western blot analysis confirming the knockdown efficiency of anillin (ANLN) in BxPC-3 cells transfected with siRNA1 and siRNA2. Knockdown of anillin resulted in increased E-cadherin levels and decreased expression of N-cadherin, Vimentin, Snail, and TGF-β, indicating inhibition of the EMT process. (A) EdU incorporation assay showing a significant reduction in proliferation in anillin-knockdown BxPC-3 cells compared to the control group (p < 0.001). (C) Transwell migration and invasion assays demonstrating a marked decrease in the migratory and invasive capabilities of anillin-knockdown BxPC-3 cells (p < 0.001). (D) Tube formation assay revealing a significant reduction in angiogenic potential following anillin knockdown (p < 0.001). All experiments were performed in triplicate, and statistical significance was determined using appropriate tests (p < 0.001). ***p < 0.001

    Journal: Frontiers in Immunology

    Article Title: Metabolic reprogramming shapes the immune microenvironment in pancreatic adenocarcinoma: prognostic implications and therapeutic targets

    doi: 10.3389/fimmu.2025.1555287

    Figure Lengend Snippet: Anillin promotes epithelial-mesenchymal transition (EMT), proliferation, migration, invasion, and angiogenesis in BxPC-3 cells. (A) Western blot analysis confirming the knockdown efficiency of anillin (ANLN) in BxPC-3 cells transfected with siRNA1 and siRNA2. Knockdown of anillin resulted in increased E-cadherin levels and decreased expression of N-cadherin, Vimentin, Snail, and TGF-β, indicating inhibition of the EMT process. (A) EdU incorporation assay showing a significant reduction in proliferation in anillin-knockdown BxPC-3 cells compared to the control group (p < 0.001). (C) Transwell migration and invasion assays demonstrating a marked decrease in the migratory and invasive capabilities of anillin-knockdown BxPC-3 cells (p < 0.001). (D) Tube formation assay revealing a significant reduction in angiogenic potential following anillin knockdown (p < 0.001). All experiments were performed in triplicate, and statistical significance was determined using appropriate tests (p < 0.001). ***p < 0.001

    Article Snippet: The siRNAs targeting anillin (si-ANLN) and the control were obtained from Sangon Biotech (Shanghai, China).

    Techniques: Migration, Western Blot, Knockdown, Transfection, Expressing, Inhibition, Control, Tube Formation Assay