Journal: Journal of Clinical Medicine
Article Title: Increased Production of Angiopoietin-like Protein 2 in a Ligature- and LPS-Induced Periodontitis Mouse Model May Promote Colorectal Tumor Progression
doi: 10.3390/jcm15062359
Figure Lengend Snippet: Histological analysis of periodontal tissue and ANGPTL2 production. ( A ) Representative hematoxylin- and eosin-stained sections from mice with and without periodontitis (scale bar: 100 µm; magnifications ×100 and ×400). ( B ) mRNA expression of ANGPTL2 and inflammatory cytokines (TNF-α, IL-1β) in gingival tissue detected by qPCR ( n = 8/group). ( C ) Immunohistochemical images using an ANGPTL2 antibody in mice with and without periodontitis (scale bar: 100 µm; magnifications ×100 and ×400). ( D ) Comparison of the number of ANGPTL2-positive cells in the periodontal tissues of control and periodontitis mice (control: n = 5; periodontitis: n = 4). Intergroup differences were analyzed using the Mann–Whitney U test. Data are presented as median and interquartile range (IQR). * p < 0.05, ** p < 0.01. AOM, azoxymethane; DSS, dextran sulfate sodium; ANGPTL2, angiopoietin-like protein 2.
Article Snippet: Immunohistochemistry was performed using anti-ANGPTL2 antibody (Proteintech Group, Chicago, IL, USA) and anti-Ki67 antibody (Abcam, Waltham, MA, USA) [ ].
Techniques: Staining, Expressing, Immunohistochemical staining, Comparison, Control, MANN-WHITNEY