Journal: Journal of Nanobiotechnology
Article Title: Dual-function MgSrCeAl-LDH nanosheets enable both ROS clearance and enhanced angiogenesis for myocardial ischemia/reperfusion injury therapy
doi: 10.1186/s12951-025-03673-8
Figure Lengend Snippet: ( a ) Schematic diagram of the animal experiment timeline. ( b ) DHE staining images of SD rats after different treatments on Day 14, Scale bar = 50 μm, n = 5. ( c ) TUNEL staining images of SD rats after different treatments on Day 14, Scale bar = 20 μm, n = 5. ( d ) Immunohistochemical staining images of C-caspase3 after different treatments on Day 14, Scale bar = 20 μm, n = 5. ( e ) EB-TTC staining images of myocardial tissue slices (thickness ~ 2 mm) from apex (left) to base (right) and ( f ) quantitative analysis of the area at risk (AAR)/left ventricular area (LV), infarct area (INF)/left ventricular area (LV), n = 4. ( g-l) Echocardiography images of cardiac function after different treatments on Day 1, Day 7, Day 14 and quantification of various ultrasonic parameters, n = 4. ( m ) Immunostaining images of CD31/Ang1 after different treatments on Day 14, Scale bar (CD31) = 50 μm, Scale bar (Ang1) = 20 μm, n ≥ 5. ( n ) Masson staining images of SD rats after different treatments on Day 14, Scale bar = 1000 μm, n = 3. ( o ) Immunohistochemical staining images of TGF-β2 after different treatments on Day 14, Scale bar = 50 μm, n = 5. Data are presented as mean values ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001
Article Snippet: Rabbit secondary antibody, mouse secondary antibody and Angiopoietin1 (Ang1) antibody were acquired from Proteintech Group (USA).
Techniques: Staining, TUNEL Assay, Immunohistochemical staining, Immunostaining
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