Journal: Redox Biology

Article Title: Triclosan exacerbates post-myocardial infarction injury via Nur77 ubiquitination: Linking NTRK2/PGC-1α-mediated mitochondrial dysfunction to senescence and ferroptosis

doi: 10.1016/j.redox.2026.104022

Figure Lengend Snippet: NTRK2 is identified as a pivotal downstream molecule mediating TCS-induced aggravation of ischemic myocardial injury. (A) Relative mRNA levels of Ntrk2 detected by RT-qPCR in heart tissues on day 3 post-MI surgery (Scale bar = 50 μm, n = 4 per group). (B) Representative immunohistochemistry images and quantitative analysis of NTRK2 expression levels in heart tissues on day 3 post-sham operation or post-MI surgery (n = 3 per group). (C) Representative fluorescence images and quantitative analyses of MitoSox staining in hypoxia-stimulated AC16 cells following exposure to DMSO, ANA-12, TCS, or co-exposure to TCS and ANA-12 (Scale bar = 20 μm, n = 3 per group). (D) Relative mRNA levels of P16 and P21 detected by RT-qPCR in hypoxia-stimulated AC16 cells following exposure to DMSO, ANA-12, TCS, or co-exposure to TCS and ANA-12 (n = 3 per group). (E) Representative Western blots and quantitative analyses of P16 and P21 in hypoxia-stimulated AC16 cells following exposure to DMSO, ANA-12, TCS, or co-exposure to TCS and ANA-12 (n = 3 per group). (F, G) Representative fluorescence images and quantitative analysis of γ-H2AX staining in hypoxia-stimulated AC16 cells across four groups (Scale bar = 10 μm, n = 3 per group). (H) Relative mRNA levels of SASP-related genes detected by RT-qPCR in hypoxia-stimulated AC16 cells across four groups, including Edn3, Gdf15, Tgfb2, and Il-1β (n = 3 per group). (I – J) Representative fluorescence images and quantitative analyses of FerroOrange staining in hypoxia-stimulated AC16 cells across four groups (Scale bar = 10 μm, n = 3 per group). (K) Relative mRNA levels of Acsl4 and Gpx4 detected by RT-qPCR in hypoxia-stimulated AC16 cells across four groups (n = 3 per group). (L) Representative Western blots and quantitative analyses of ACSL4 and GPX4 in hypoxia-stimulated AC16 cells across four groups (n = 3 per group). (M) Representative echocardiographic images and quantitative analyses of EF% and FS% in MI mice after 8 weeks of TCS exposure with/without ANA-12 treatment (n = 4 per group). (N) Representative Western blots and quantitative analyses of ACSL4, GPX4, P16, and P21 in MI mice 3 days post-infarction after 8 weeks of TCS exposure with/without ANA-12 treatment (n = 4 per group). (O) Representative Western blots and quantitative analyses of α-SMA and Col3a1 in MI mice 21 days post-infarction after 8 weeks of TCS exposure with/without ANA-12 treatment (n = 4 per group). Data are presented as mean ± SD. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001. TCS, Triclosan; RT-qPCR, Quantitative Reverse Transcription Polymerase Chain Reaction; MI, Myocardial Infarction; MitoSox, Red Mitochondrial Superoxide Indicator; DMSO, Dimethyl Sulfoxide; SASP, Senescence-associated Secretory Phenotype; EF, Ejection Fraction; FS, Fractional Shortening.

Article Snippet: TCS (HY–B1119), ANA-12 (HY-12497), ZLN005 (HY-17538), SR-18292 (HY-101491), LY294002 (HY-10108), MG-132 (HY-13259) and chloroquine (CQ, HY-17589A) were purchased from MedChemExpress Co., Ltd. (Shanghai, China).

Techniques: Quantitative RT-PCR, Immunohistochemistry, Expressing, Fluorescence, Staining, Western Blot, Reverse Transcription, Polymerase Chain Reaction