Journal: GeroScience

Article Title: A novel synthetic 3,4,5-tri-feruloylquinic acid enhances learning and memory via neurotrophin signaling in an aging model senescence-accelerated prone 8 mice

doi: 10.1007/s11357-025-01783-7

Figure Lengend Snippet: Effects of TFQA on modulation of TrkB, the primary receptor for BDNF. A The Biacore assay, a label-free, real-time surface plasmon resonance (SPR) technique, was used to assess the binding affinity of TFQA to TrkB. Binding response values at varying concentrations of TFQA indicate a dose-dependent interaction. B Cell activity of SH-SY5Y cells treated with increasing concentrations of TFQA for 24 and 48 h, evaluated using MTT assay ( n = 4 per group). C BDNF mRNA expression levels in SH-SY5Y cells following treatment with vehicle control, ANA-12 (a TrkB antagonist), 10 µM TFQA, and 10 µM TFQA combined with ANA-12 for 24 h ( n = 3 in each group). Data are expressed as the mean ± SD of three independent experiments. *Compared with control, # compared with TFQA-treated group, ** P < 0.01 and #### P < 0.0001 by ANOVA followed by Tukey’s post hoc test

Article Snippet: The cells were treated with 10 and 20 μM TFQA and co-treated with the inhibitor, 10 μM ANA-12 (Selleck Biotech, Japan), as a TrkB antagonist, for an additional 24 h.

Techniques: SPR Assay, Binding Assay, Activity Assay, MTT Assay, Expressing, Control

Journal: Science translational medicine

Article Title: Epstein-Barr virus reprograms autoreactive B cells as antigen presenting cells in systemic lupus erythematosus

doi: 10.1126/scitranslmed.ady0210

Figure Lengend Snippet: ( A ) Representative examples of immunostaining patterns of SLE EBV + B cell-derived mAb on HEp-2 cells. Nuclear homogeneous includes both homogeneous and fine speckled staining patterns. ( B ) Representative examples of immunostaining of HC and MS EBV + B cell-derived mAbs on HEp-2 cells. ( C ) ELISA analysis of the reactivity of SLE, HC, and MS EBV + B cell-derived mAbs to prototypical SLE autoantigens, including the ANA screen ELISA (Nuclear Ag cocktail) and individual nuclear antigens, and for EBV EBNA1. As an assessment for potential polyreactive antibodies, LPS ELISAs were performed. The Nuclear Antigen (Ag) cocktail, comprising Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52 kDa and 60 kDa), SS-B (La), Jo-1, U1-SmRNP, CENP-B, dsDNA, Histones, and C1q was obtained from a commercial source different from that used for the individual antigens in the heatmap. N = number of mAbs tested per group.

Article Snippet: The nuclear antigen cocktail ELISA was performed according to the manufacturer’s instructions using the ANA Screen ELISA kit (Eagle Biosciences, Cat# 4010), which included a cocktail of SLE antigens including Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52kDa and 60kDa), SS-B (La), Jo1, U1-SmRNP, CENP-B, dsDNA and Histones.

Techniques: Immunostaining, Immunopeptidomics, Derivative Assay, Staining, Enzyme-linked Immunosorbent Assay