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william sellers  (Addgene inc)


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    Structured Review

    Addgene inc william sellers
    William Sellers, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/akt3/1236+pcDNA3+Myr+HA+Akt3+(Plasmid+%239017)/us12533423-484-21-23
    Average 93 stars, based on 17 article reviews
    william sellers - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Specific Akt Family Members Impair Stress-Mediated Transactivation of Viral Promoters and Enhance Neuronal Differentiation: Important Functions for Maintaining Latency
    Article Snippet: Neuro-2A cells 318 A plasmid that expresses Akt1 was a gift from Jie Chen (pCDNA3-HA-Akt1; plasmid 319 73408; Addgene). .. A plasmid that expresses Akt3 was a gift from William Sellers (1236 320 pcDNA3 Myr HA Akt3; plasmid 9017; Addgene). .. A plasmid that expresses Akt2 was a gift 321 from William Sellers (1227 pcDNA3 Myr HA Akt2; plasmid # 9016; Addgene).

    Article Title: Akt3-Mediated Resistance to Apoptosis in B-RAF–Targeted Melanoma Cells
    Article Snippet: .. Cells transfected for 72 hours before subsequent treatment lysis. ation of lentiviruses and cell lines d-type Akt3 and NH2-terminal myristylated Akt3 were ied from human Akt3 cDNA plasmid (Addgene) using OD Hot Start DNA polymerase kit (Novagen). ..

    Article Title: The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency
    Article Snippet: A plasmid that expresses 471 Akt1 was a gift from Dr. Jie Chen (pCDNA3-HA-Akt1; Addgene plasmid # 73408). .. A plasmid 472 that expresses Akt3 was a gift from Dr. William Sellers (1236 pcDNA3 Myr HA Akt3; Addgene 473 plasmid # 9017). .. 474 All plasmids were transfected into Neuro-2A cells in 60 mm dishes using 475 Lipofectamine® 3000 Transfection Reagent (L3000075, Invitrogen) according to the 476 manufacturers’ instructions.

    Expressing:

    Article Title: Expression Signatures of the Lipid-Based Akt Inhibitors Phosphatidylinositol Ether Lipid Analogues in NSCLC Cells
    Article Snippet: .. Cell lines expressing Akt isoform–specific short hairpin RNA (shRNA) were created by lentiviral infection and shRNA vectors used were from Sigma-Aldrich unless otherwise noted: Akt1, NM_005163.1-628s1c1; Akt2, NM_001626.2-1509s1c1; Akt3, NM_005465.3-671s1c1; nontargeting, pLKO-scr (Addgene). ..

    Article Title: An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.
    Article Snippet: .. To establish Myr Kinases stable cell lines on top of the NELFB-AID parental cells, cells were infected with retroviruses expressing the following kinases: AKT1, PKM2, RPS6KB1, AKT3, PIK3CB, PIK3CG, CDK9, MAP2K7, PAK4, PDK1, and NTRK3 (Addgene, 1000000012). ..

    shRNA:

    Article Title: Expression Signatures of the Lipid-Based Akt Inhibitors Phosphatidylinositol Ether Lipid Analogues in NSCLC Cells
    Article Snippet: .. Cell lines expressing Akt isoform–specific short hairpin RNA (shRNA) were created by lentiviral infection and shRNA vectors used were from Sigma-Aldrich unless otherwise noted: Akt1, NM_005163.1-628s1c1; Akt2, NM_001626.2-1509s1c1; Akt3, NM_005465.3-671s1c1; nontargeting, pLKO-scr (Addgene). ..

    Infection:

    Article Title: Expression Signatures of the Lipid-Based Akt Inhibitors Phosphatidylinositol Ether Lipid Analogues in NSCLC Cells
    Article Snippet: .. Cell lines expressing Akt isoform–specific short hairpin RNA (shRNA) were created by lentiviral infection and shRNA vectors used were from Sigma-Aldrich unless otherwise noted: Akt1, NM_005163.1-628s1c1; Akt2, NM_001626.2-1509s1c1; Akt3, NM_005465.3-671s1c1; nontargeting, pLKO-scr (Addgene). ..

    Article Title: An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.
    Article Snippet: .. To establish Myr Kinases stable cell lines on top of the NELFB-AID parental cells, cells were infected with retroviruses expressing the following kinases: AKT1, PKM2, RPS6KB1, AKT3, PIK3CB, PIK3CG, CDK9, MAP2K7, PAK4, PDK1, and NTRK3 (Addgene, 1000000012). ..

    Stable Transfection:

    Article Title: An essential signaling function of cytoplasmic NELFB is independent of RNA polymerase II pausing.
    Article Snippet: .. To establish Myr Kinases stable cell lines on top of the NELFB-AID parental cells, cells were infected with retroviruses expressing the following kinases: AKT1, PKM2, RPS6KB1, AKT3, PIK3CB, PIK3CG, CDK9, MAP2K7, PAK4, PDK1, and NTRK3 (Addgene, 1000000012). ..

    Transfection:

    Article Title: Akt3-Mediated Resistance to Apoptosis in B-RAF–Targeted Melanoma Cells
    Article Snippet: .. Cells transfected for 72 hours before subsequent treatment lysis. ation of lentiviruses and cell lines d-type Akt3 and NH2-terminal myristylated Akt3 were ied from human Akt3 cDNA plasmid (Addgene) using OD Hot Start DNA polymerase kit (Novagen). ..

    Lysis:

    Article Title: Akt3-Mediated Resistance to Apoptosis in B-RAF–Targeted Melanoma Cells
    Article Snippet: .. Cells transfected for 72 hours before subsequent treatment lysis. ation of lentiviruses and cell lines d-type Akt3 and NH2-terminal myristylated Akt3 were ied from human Akt3 cDNA plasmid (Addgene) using OD Hot Start DNA polymerase kit (Novagen). ..



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