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Effects of Heated <t>AITC</t> on hTRPA1 Activity A, B) Typical traces, current-voltage curves, and graph of temperature changes of HEK293T cells expressing hTRPA1 in response to room temperature (A) and heating (B) AITC. The holding potentials were −60 mV with ramp pulses (−100∼+100 mV, 300 ms). The insets show current-voltage curves at the time points of a, b, and c. C) The plot of hTRPA1 current density in response to AITC application over the range of about 25 °C–45 °C.
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Effects of Heated AITC on hTRPA1 Activity A, B) Typical traces, current-voltage curves, and graph of temperature changes of HEK293T cells expressing hTRPA1 in response to room temperature (A) and heating (B) AITC. The holding potentials were −60 mV with ramp pulses (−100∼+100 mV, 300 ms). The insets show current-voltage curves at the time points of a, b, and c. C) The plot of hTRPA1 current density in response to AITC application over the range of about 25 °C–45 °C.

Journal: Biochemistry and Biophysics Reports

Article Title: Propofol reduces human TRPA1 activity in a warm environment

doi: 10.1016/j.bbrep.2025.101918

Figure Lengend Snippet: Effects of Heated AITC on hTRPA1 Activity A, B) Typical traces, current-voltage curves, and graph of temperature changes of HEK293T cells expressing hTRPA1 in response to room temperature (A) and heating (B) AITC. The holding potentials were −60 mV with ramp pulses (−100∼+100 mV, 300 ms). The insets show current-voltage curves at the time points of a, b, and c. C) The plot of hTRPA1 current density in response to AITC application over the range of about 25 °C–45 °C.

Article Snippet: Allyl isothiocyanate (AITC) was purchased from Tokyo Chemical Industry Co., Ltd. Stock solutions were prepared in DMSO; the final concentration of DMSO did not exceed 0.3 %.

Techniques: Activity Assay, Expressing