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afai  (TaKaRa)


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  • 93

    Structured Review

    TaKaRa afai
    Afai, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/afai/Afa+I/pm41318656-54-13-14
    Average 93 stars, based on 131 article reviews
    afai - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase R.CcoLI from Campylobacter coli
    Article Snippet: The DNAs were visualized with blue-LED light after GelGreen (Biotium) staining. .. Products by AfaI (TAKARA), which cleaves the sequence 5′-GTAC-3′, were also separated as a control. ..

    Article Title: Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase R.CcoLI from Campylobacter coli.
    Article Snippet: The DNAs were visualized with blue-LED light after GelGreen (Biotium) staining. .. Products by AfaI (TAKARA), which cleaves the sequence 5′-GTAC-3′, were also separated as a control. ..

    Control:

    Article Title: Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase R.CcoLI from Campylobacter coli
    Article Snippet: The DNAs were visualized with blue-LED light after GelGreen (Biotium) staining. .. Products by AfaI (TAKARA), which cleaves the sequence 5′-GTAC-3′, were also separated as a control. ..

    Article Title: Crystal structure and DNA cleavage mechanism of the restriction DNA glycosylase R.CcoLI from Campylobacter coli.
    Article Snippet: The DNAs were visualized with blue-LED light after GelGreen (Biotium) staining. .. Products by AfaI (TAKARA), which cleaves the sequence 5′-GTAC-3′, were also separated as a control. ..

    Polymerase Chain Reaction:

    Article Title: The polymorphism of bovine Cofilin-1 gene sequence variants and association analysis with growth traits in Qinchuan cattle.
    Article Snippet: In our study, four single nucleotide polymorphisms (SNPs) were identified in exon 2 of cofilin-1 (CFL1) gene in 488 Chinese Qinchuan (QC) cattle, which included two missense mutations T 2084G and G 2107C, two synonymous mutations T 2052C and T 2169C.. Further, we evaluated haplotype frequency and linkage disequilibrium (LD) coefficient of four SNPs.. At SNP T 2052C, G 2107C and T 2169C, the QC cattle population belonged to intermediate genetic diversity (0.25 < PIC-value < 0.5), whereas SNP T-2084G belonged to low polymorphism (PIC-value < 0.25).

    Article Title: Soil microbial biodiversity supports the delivery of multiple ecosystem functions under elevated CO2 and warming
    Article Snippet: PCR products were purified using PCR Fragment Purification Kit (Takara). .. Purified PCR products were used to restriction digestion using HhaI (Takara, Dalian, China) for bacterial and fungal genes, and AfaI (Takara, Dalian, China) for archaeal genes following the manufacturer’s guidelines. .. Communications Earth & Environment | (2024) 5:615 6 Fragment analysis was performed using capillary electrophoresis on anABI 3100 Genetic Analyzer (Applied Biosystems, CA), with a GeneScan ROXlabeledGS500 internal size standard.

    Article Title: Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility
    Article Snippet: .. PCR products were digested with AfaI and MspI. (TaKaRa, Kusatsu, Shiga, Japan) for 37 reactions per sample for 3 hours at 2°C,. ..

    Article Title: Gut microorganisms of Locusta migratoria in various life stages and its possible influence on cellulose digestibility.
    Article Snippet: .. PCR products were digested with AfaI and MspI. (TaKaRa, Kusatsu, Shiga, Japan) for 37 reactions per sample for 3 hours at 2°C,. ..

    Article Title: A novel restriction fragment length polymorphism (RFLP) -based method for rapid genotyping of bovine coronaviruses.
    Article Snippet: Outbreaks of diarrhea and respiratory symptoms caused by bovine coronavirus (BCoV) have been reported worldwide, leading to significant economic losses.. It is important to genetically analyze the spike (S) gene of this virus as it is closely linked to its antigenicity and virulence, in order to quickly understand the molecular characteristics of circulating BCoVs.. A previous study showed that BCoVs can be easily genotyped using reverse transcription (RT)-polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) analysis using AvaII and EcoO65I enzymes targeted on the polymorphic region in the S1 subunit of the S gene.

    Purification:

    Article Title: Soil microbial biodiversity supports the delivery of multiple ecosystem functions under elevated CO2 and warming
    Article Snippet: PCR products were purified using PCR Fragment Purification Kit (Takara). .. Purified PCR products were used to restriction digestion using HhaI (Takara, Dalian, China) for bacterial and fungal genes, and AfaI (Takara, Dalian, China) for archaeal genes following the manufacturer’s guidelines. .. Communications Earth & Environment | (2024) 5:615 6 Fragment analysis was performed using capillary electrophoresis on anABI 3100 Genetic Analyzer (Applied Biosystems, CA), with a GeneScan ROXlabeledGS500 internal size standard.



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