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adsc human adipose derived stem cell growth bulletkit medium  (Lonza)


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    Structured Review

    Lonza adsc human adipose derived stem cell growth bulletkit medium
    Adsc Human Adipose Derived Stem Cell Growth Bulletkit Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adsc-bulletkit/adipose+derived+stem+cell+growth+bulletkit+medium/pm40259783-132-4-13
    Average 90 stars, based on 1 article reviews
    adsc human adipose derived stem cell growth bulletkit medium - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Derivative Assay:

    Article Title: Trophic factor releasing agent and inflammatory disease treating agent
    Article Snippet: Human adipose derived stem cells (hADSC) were suspended in a proliferation medium (Lonza: ADSC-BulletKit (Trademark), hereinafter, also referred to as an ADSC medium), biocompatible polymer blocks (53 to 106 μm) manufactured in Example 3 were added and sown in EZSPHERE (registered trademark) DISH 35 mm Type 903 (which had a spheroid well diameter of 800 μm, a spheroid well depth of 300 μm, and several spheroid wells to about 1,000 wells, and was manufactured by AGC Techno Glass Co., Ltd.) which was a cell non-adhesive 35 mm dish having recessed portions on the bottom surface, in a state in which hADSCs (1.2×106 cells) and biocompatible polymer blocks (1 mg) were suspended in 4 mL of a medium.

    Article Title: Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells
    Article Snippet: Primary ADSCs on days 6 and 13 of culture and iADSCs on day 64 of culture were seeded at 3.0 × 10 3 cells per well in a 96-well plate and cultured in ADSC-BulletKit (Lonza, Basel, Switzerland).

    Article Title: Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells
    Article Snippet: After each well was filled with ADSC-BulletKit (Lonza), plates were degassed in a vacuum desiccator to remove air bubbles from the sheets and then preincubated for 1 h in a humidified incubator with 5% CO 2 . iADSCs-mCherry-nls (4 × 10 4 ) cells were added per well and cultured at 33 °C for 7–14 days.

    Polymer:

    Article Title: Trophic factor releasing agent and inflammatory disease treating agent
    Article Snippet: Human adipose derived stem cells (hADSC) were suspended in a proliferation medium (Lonza: ADSC-BulletKit (Trademark), hereinafter, also referred to as an ADSC medium), biocompatible polymer blocks (53 to 106 μm) manufactured in Example 3 were added and sown in EZSPHERE (registered trademark) DISH 35 mm Type 903 (which had a spheroid well diameter of 800 μm, a spheroid well depth of 300 μm, and several spheroid wells to about 1,000 wells, and was manufactured by AGC Techno Glass Co., Ltd.) which was a cell non-adhesive 35 mm dish having recessed portions on the bottom surface, in a state in which hADSCs (1.2×106 cells) and biocompatible polymer blocks (1 mg) were suspended in 4 mL of a medium.

    Article Title: Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells
    Article Snippet: Primary ADSCs on days 6 and 13 of culture and iADSCs on day 64 of culture were seeded at 3.0 × 10 3 cells per well in a 96-well plate and cultured in ADSC-BulletKit (Lonza, Basel, Switzerland).

    Article Title: Development of a Mouse Experimental System for the In Vivo Characterization of Bioengineered Adipose-Derived Stromal Cells
    Article Snippet: After each well was filled with ADSC-BulletKit (Lonza), plates were degassed in a vacuum desiccator to remove air bubbles from the sheets and then preincubated for 1 h in a humidified incubator with 5% CO 2 . iADSCs-mCherry-nls (4 × 10 4 ) cells were added per well and cultured at 33 °C for 7–14 days.



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    Encapsulated HUVECs <t>and</t> <t>hADSCs</t> in 3D hydrogels. (A) Day 14 LIVE/DEAD imaging of encapsulated cells. The images are displayed in Z-projection and merged channels (Green: live cells, red: dead cells, blue: nuclei, scale bar: 100 μm). (B) CCK-8 assay cell growth profile of GelMA, GelMA@VEGF 165 , and VEGF@bFGF in days 1, 3, 7, 14, and 28 of coculture in complete <t>ADSC</t> and complete M199:ADSC (50:50) medium.
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    Encapsulated HUVECs <t>and</t> <t>hADSCs</t> in 3D hydrogels. (A) Day 14 LIVE/DEAD imaging of encapsulated cells. The images are displayed in Z-projection and merged channels (Green: live cells, red: dead cells, blue: nuclei, scale bar: 100 μm). (B) CCK-8 assay cell growth profile of GelMA, GelMA@VEGF 165 , and VEGF@bFGF in days 1, 3, 7, 14, and 28 of coculture in complete <t>ADSC</t> and complete M199:ADSC (50:50) medium.
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    Image Search Results


    Encapsulated HUVECs and hADSCs in 3D hydrogels. (A) Day 14 LIVE/DEAD imaging of encapsulated cells. The images are displayed in Z-projection and merged channels (Green: live cells, red: dead cells, blue: nuclei, scale bar: 100 μm). (B) CCK-8 assay cell growth profile of GelMA, GelMA@VEGF 165 , and VEGF@bFGF in days 1, 3, 7, 14, and 28 of coculture in complete ADSC and complete M199:ADSC (50:50) medium.

    Journal: ACS Biomaterials Science & Engineering

    Article Title: Enhanced Vascular-like Network Formation of Encapsulated HUVECs and ADSCs Coculture in Growth Factors Conjugated GelMA Hydrogels

    doi: 10.1021/acsbiomaterials.4c00465

    Figure Lengend Snippet: Encapsulated HUVECs and hADSCs in 3D hydrogels. (A) Day 14 LIVE/DEAD imaging of encapsulated cells. The images are displayed in Z-projection and merged channels (Green: live cells, red: dead cells, blue: nuclei, scale bar: 100 μm). (B) CCK-8 assay cell growth profile of GelMA, GelMA@VEGF 165 , and VEGF@bFGF in days 1, 3, 7, 14, and 28 of coculture in complete ADSC and complete M199:ADSC (50:50) medium.

    Article Snippet: All experiments were performed with HUVECs between passages 5 to 14. hADSCs were sourced from POIETICS and were cultured in complete ADSC medium (ADSC Growth Media BulletKit, Lonza, Switzerland).

    Techniques: Imaging, CCK-8 Assay

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Integration of transcriptomes of senescent cell models with multi-tissue patient samples reveals reduced COL6A3 as an inducer of senescence

    doi: 10.1016/j.celrep.2023.113371

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ADSC Bulletkit , Lonza , PT-4505.

    Techniques: Imaging, Recombinant, Biomarker Discovery, SYBR Green Assay, Control, shRNA, Plasmid Preparation, Fluorescence, Software, Gene Expression