proteintech adipor1  (Proteintech)

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: (A, B) Bar‐graph demonstrating the expression of (A) biomarkers and (B) their receptors in endometrial cancer tissue ( n = 39). X axis‐ biomarkers (A), receptors (B). Y axis–expressions of biomarkers as fold change over benign endometrial calibrator sample (assigned value = 1, for comparison with the cancer samples). The error bars represent standard errors of mean. 1 (C) Heat map demonstrating the expression of all 6 biomarkers and their receptors in endometrial cancer tissues ( n = 39). AdipoQ, adiponectin; ADIPOR1/R2, adiponectin receptors; OBR, leptin receptor; TNF1A, TNFRSF1A; TNF1B, TNFRSF1B (TNF receptors). Gene expressions are plotted on the Y ‐axis against the individual patients on the X ‐axis. Gene expressions are plotted as fold changes over a single pooled calibrator sample value. The color scale ranges from black (lowest value) to white (median) to yellow (highest value).

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Expressing, Comparison

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: Heat map comparing the expression of (A) adiponectin, leptin and their receptors in endometrial cancer tissue and parametrial adipose tissue ( n = 39) (B) all 6 biomarkers and their receptors in endometrial cancer tissue and lymph nodes ( n = 12). AdipoQ, adiponectin; AdipoR1/R2, adiponectin receptors; ObR, leptin receptor; TNF1A, TNFRSF1A; TNF1B, TNFRSF1B (TNF receptors). Gene expressions are plotted on the Y ‐axis against the individual patients on the X ‐axis. Gene expression plotted as fold changes over a single pooled calibrator sample. The color scale ranges from black (lowest value) to white (median) to yellow (highest value).

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Expressing, Gene Expression

Journal: Cancer Medicine

Article Title: Exploring the Relationship Between Adipocytokines and Endometrial Cancer: Identifying Correlations With Clinico‐Pathological Prognostic Factors

doi: 10.1002/cam4.71007

Figure Lengend Snippet: Illustration of chromogenic 3,3′‐Diaminobenzidine (DAB) staining for ADIPOR1 (A) and ADIPOR2 (B) receptor in endometrial cancer tissue and benign tissues. Image taken at 10× magnification, Scalebar = 100 μm.

Article Snippet: The antibodies used were: ADIPOR1 (Proteintech, Manchester, UK, #66619‐1‐Ig; anti‐mouse antibody) and ADIPOR2 (Abcam, Cambridge, UK, #ab223752; anti‐rabbit antibody).

Techniques: Staining

Journal: Frontiers in Neurology

Article Title: Unlocking amyotrophic lateral sclerosis: the role of adiponectin in inflammation and disease progression

doi: 10.3389/fneur.2025.1605822

Figure Lengend Snippet: The levels of AdipoR1, AdipoR2, IL1β, and IL-10 in monocytes and monocyte-derived macrophages isolated from the blood of patients with ALS. (A–C) Results of flow cytometry for the expression levels of AdipoR1 and AdipoR2 on monocytes obtained from controls and ALS patients. Data represent the mean ± SEM. Statistical analysis was performed with an unpaired t -test, * P < 0.05; ** P < 0.01. (D) Immunocytochemistry labeling for the expression of AdipoR1, AdipoR2, IL-1β, and IL-10 on macrophages from controls and patients with ALS (Scale bar= 20 μm). Data represent the mean ± SEM, n = 10 per group. Statistical analysis was performed with an unpaired t -test, * P < 0.05. ALS, amyotrophic lateral sclerosis; AdipoR1, Adiponectin receptor 1; AdipoR2, Adiponectin receptor 2.

Article Snippet: The primary and secondary antibody used in this study were as follows: MHC-II (1:200, Abcam, Waltham, MA, USA; ab23990), CD206 (1:100, R&D Systems, Minnesota, USA; AF2535), IL-1β (1:200, proteintech, Wuhan, China; 16806-1-AP), IL-10 (1:200, Invitrogen, California, USA; 16-7108-81), AdipoR1 (1:400, Santa Cruz, CA, USA; sc-518030), AdipoR2 (1:400, Santa Cruz, CA, USA; sc-514045), donkey anti-mouse IgG H&L 647 (1:1000, Abcam, Waltham, MA, USA; ab150111), donkey anti-rabbit IgG H&L 594 (1:1000, Invitrogen, Shanghai, China; A21207), donkey anti-rat IgG H&L 488 (1:1000, Invitrogen, Shanghai, China; A21208), donkey anti-goat IgG H&L 647 (1:1000, Invitrogen, Shanghai, China; A21447).

Techniques: Derivative Assay, Isolation, Flow Cytometry, Expressing, Immunocytochemistry, Labeling

Journal: Frontiers in Neurology

Article Title: Unlocking amyotrophic lateral sclerosis: the role of adiponectin in inflammation and disease progression

doi: 10.3389/fneur.2025.1605822

Figure Lengend Snippet: Inflammation and polarization of macrophages induced by AdipoRon. (A–E) Immunocytochemistry labeling and quantitative analysis of the fluorescence intensity for the expression of AdipoR1, IL-1β, IL-10, MHC-II, and CD206 on macrophages from patients with ALS, respectively (Scale bar = 20 μm). (F, G) Levels of IL-1β and IL-10 in the cell culture supernatants from patients with ALS under different interventions were determined by ELISA. (H) Schematic diagram of experimental design for investigate the impact of AdipoRon on macrophages polarization. Data represent the mean ± SEM, n = 10 per group. Statistical analysis was performed with one-way ANOVA, * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. AdipoR1, Adiponectin receptor 1; AdipoRon, Adiponectin receptor agonists; PBMC, peripheral blood mononuclear cell; ALS, amyotrophic lateral sclerosis; M-CSF, macrophage colony-stimulating factor.

Article Snippet: The primary and secondary antibody used in this study were as follows: MHC-II (1:200, Abcam, Waltham, MA, USA; ab23990), CD206 (1:100, R&D Systems, Minnesota, USA; AF2535), IL-1β (1:200, proteintech, Wuhan, China; 16806-1-AP), IL-10 (1:200, Invitrogen, California, USA; 16-7108-81), AdipoR1 (1:400, Santa Cruz, CA, USA; sc-518030), AdipoR2 (1:400, Santa Cruz, CA, USA; sc-514045), donkey anti-mouse IgG H&L 647 (1:1000, Abcam, Waltham, MA, USA; ab150111), donkey anti-rabbit IgG H&L 594 (1:1000, Invitrogen, Shanghai, China; A21207), donkey anti-rat IgG H&L 488 (1:1000, Invitrogen, Shanghai, China; A21208), donkey anti-goat IgG H&L 647 (1:1000, Invitrogen, Shanghai, China; A21447).

Techniques: Immunocytochemistry, Labeling, Fluorescence, Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Neurology

Article Title: Unlocking amyotrophic lateral sclerosis: the role of adiponectin in inflammation and disease progression

doi: 10.3389/fneur.2025.1605822

Figure Lengend Snippet: AdipoRon-mediated polarization of M2 macrophages affects neuronal proliferation and apoptosis. (A, B) Cell proliferation curve and the cell-value-added rate of NSC34-E cells under different treatments. (C, D) Cell apoptosis curve and the cell-value-apoptosis rate of NSC34-E cells under different treatments. (E, F) Cell proliferation curve and the cell-value-added rate of NSC34-hSOD1 G93A cells under different treatments. (G, H) Cell apoptosis curve and the cell-value-apoptosis rate of NSC34-hSOD1 G93A cells under different treatments. Data represent the mean ± SEM, n = 5 per group. Statistical analysis was performed with one-way ANOVA, * P < 0.05; ** P < 0.01. AdipoRon, Adiponectin receptor agonists; AdipoR1, Adiponectin receptor 1; E, NSC34-E cell; G93A, NSC34-hSOD1 G93A cells.

Article Snippet: The primary and secondary antibody used in this study were as follows: MHC-II (1:200, Abcam, Waltham, MA, USA; ab23990), CD206 (1:100, R&D Systems, Minnesota, USA; AF2535), IL-1β (1:200, proteintech, Wuhan, China; 16806-1-AP), IL-10 (1:200, Invitrogen, California, USA; 16-7108-81), AdipoR1 (1:400, Santa Cruz, CA, USA; sc-518030), AdipoR2 (1:400, Santa Cruz, CA, USA; sc-514045), donkey anti-mouse IgG H&L 647 (1:1000, Abcam, Waltham, MA, USA; ab150111), donkey anti-rabbit IgG H&L 594 (1:1000, Invitrogen, Shanghai, China; A21207), donkey anti-rat IgG H&L 488 (1:1000, Invitrogen, Shanghai, China; A21208), donkey anti-goat IgG H&L 647 (1:1000, Invitrogen, Shanghai, China; A21447).

Techniques: