Journal: Journal of Inflammation Research
Article Title: The m6A/m1A/m5C-Related Methylation Modification Patterns and Immune Landscapes in Rheumatoid Arthritis and Osteoarthritis Revealed by Microarray and Single-Cell Transcriptome
doi: 10.2147/JIR.S431076
Figure Lengend Snippet: Validation of biomarkers. ( A ) IHC for ADAMDEC1 and IGHM , as well as HE staining and its scoring on RA and OA synovial tissues (n = 10 per group). ( B ) HE staining, Safranin-O/Fast Green staining, and Toluidine Blue staining of the sham group and DMM group. ( C ) IHC of cartilage metabolic biomarkers. ( D ) OARSI score of knee joints in each group of mice. ( E ) IHC of OGN, TNFRSF11B, SCARA3 , and PTN in the sham group and DMM group (n = 6 per group). Scale bar = 200μm, * P < 0.05, ** P < 0.01, and **** P < 0.0001.
Article Snippet: For immunostaining of ADAMDEC1 and IGHM , the following primary antibodies were utilized: rabbit anti-ADAMDEC1 (Proteintech, Wuhan, China; cat. no. 17899-1-AP, 1:200) and mouse anti-IGHM (Proteintech, Wuhan, China; cat. no. 66484-1-Ig, 1:3000).
Techniques: Biomarker Discovery, Staining