acvr1c  (Proteintech)

Journal: JBMR Plus

Article Title: An inducible knock-in mouse model of fibrodysplasia ossificans progressiva shows spontaneous formation of heterotopic ossification

doi: 10.1093/jbmrpl/ziaf123

Figure Lengend Snippet: Acvr1 ARC-R206H/+ ;R26 creERT2/creERT2 mice. (A) Genetic construct of Acvr1 ARC-R206H/+ knock-in allele (see text for additional description). (B) Experimental design showing the dosing and timing of Tamoxifen administration and assay time points. (C) Representative microCT images (VivaCT80) to detect mineralized bone at indicated days post-injury (dpi) by CTX injection. Arrow points to HO. n = 3 per time point. Scale bar = 5 mm. (D) Quantification of HO bone volume. Mean ± SEM displayed. n = 3. (E) Representative images of hind limb sections stained with H&E, Alcian Blue, and Orange G at indicated time points show immune cell infiltration (I) at 3 dpi, fibroproliferation (FP) at 7 dpi, and hypertrophic chondrocytes (C) with adjacent regions of (B) maturing heterotopic bone at 21 dpi. Scale bar = 50 μm for low magnification images (4×, top panel); 10 μm for high magnification images (40×, lower panel) (i-iii). Abbreviations: F, fibula; G, gastrocnemius; FP, fibroproliferation; C, chondrocytes; B, bone.

Article Snippet: A total of 3 doses were given via intraperitoneal (i.p.) injections every 2 d. Acvr1 ARC-R206H/+ mice were crossed with mice double transgenic for Rosa26-rtTA and tetO-Cre (Gt(ROSA)26Sortm1(rtTA*M2)Jae and Tg(tetO-Cre)1Jaw; Jackson Laboratories 006965 and 006224) that express Cre recombinase in response to doxycycline.

Techniques: Construct, Knock-In, Injection, Staining

Journal: JBMR Plus

Article Title: An inducible knock-in mouse model of fibrodysplasia ossificans progressiva shows spontaneous formation of heterotopic ossification

doi: 10.1093/jbmrpl/ziaf123

Figure Lengend Snippet: Acvr1 ARC-R206H/+ ;rt/rt;tetO-Cre mice. (A) Experimental design showing timing of doxycycline induction and assay time points. (B) Quantification of Acvr1 ARC-R206H/+ allele recombination from tail snips of mice after 5 d of doxycycline treatment. n = 15. (C) MicroCT images (VivaCT40) at indicated days post-injury (dpi) by CTX injection. Arrows point to HO. n = 3-5 per time point. Scale bar = 5 mm. (D) Quantification of heterotopic ossification (HO) bone volume. Graph represents the mean ± SEM. n = 3-5 per timepoint. (E) Representative images of hind limb sections stained with H&E, Alcian Blue, and Orange G at indicated time points showing (i) immune cell infiltration (I) at 3 dpi, (ii) fibroproliferation (FP) at 6 dpi), (iii) hypertrophic chondrocytes (C) at 10 dpi, and (iv) mature heterotopic bone (B) at 14 dpi. Scale bar = 50 μm for low magnification images (top panel); 10 μm for high magnification images (i-iv). Abbreviations: F, fibula; G, gastrocnemius; FP, fibroproliferation; C, chondrocytes; B, bone.

Article Snippet: A total of 3 doses were given via intraperitoneal (i.p.) injections every 2 d. Acvr1 ARC-R206H/+ mice were crossed with mice double transgenic for Rosa26-rtTA and tetO-Cre (Gt(ROSA)26Sortm1(rtTA*M2)Jae and Tg(tetO-Cre)1Jaw; Jackson Laboratories 006965 and 006224) that express Cre recombinase in response to doxycycline.

Techniques: Injection, Staining

Journal: JBMR Plus

Article Title: An inducible knock-in mouse model of fibrodysplasia ossificans progressiva shows spontaneous formation of heterotopic ossification

doi: 10.1093/jbmrpl/ziaf123

Figure Lengend Snippet: Acvr1 ARC-R206H/+ ;rt/rt;tetO-Cre mice acquire non-injury induced HO. (A) Experimental design indicating timing of doxycycline induction and assay time points. (B) Whole-body microCT representative images (VivaCT40) 2, 3, and 4 wk after induction of mutation expression. Arrows point to HO. n = 3-5 per time point. Scale bar = 5 mm. (C) Quantification of HO volume at times post mutation induction. Data are plotted as mean ± SEM. n = 3-5. (D) Representative image stained with H&E, Alcian Blue, and Orange G at 4 wk post-mutation expression shows an example of new bone formation adjacent to spine with (i) cartilage formation (C) within skeletal muscle (M) and (ii) hypertrophic chondrocytes and new mineralized bone (B). Scale bar = 50 μm for low magnification image (4×, left panel); 10 μm for high magnification images (i and ii) (40×, right panels). Abbreviations: M, skeletal muscle; C, chondrocytes; B, bone.

Article Snippet: A total of 3 doses were given via intraperitoneal (i.p.) injections every 2 d. Acvr1 ARC-R206H/+ mice were crossed with mice double transgenic for Rosa26-rtTA and tetO-Cre (Gt(ROSA)26Sortm1(rtTA*M2)Jae and Tg(tetO-Cre)1Jaw; Jackson Laboratories 006965 and 006224) that express Cre recombinase in response to doxycycline.

Techniques: Mutagenesis, Expressing, Staining