Journal: Journal of Translational Medicine
Article Title: Macrophage-derived IL-6 reprograms lipid metabolism to promote colorectal cancer development through USP14-mediated FASN deubiquitination
doi: 10.1186/s12967-026-07911-x
Figure Lengend Snippet: FASN-mediated lipogenesis in CRC cells was upregulated by macrophages. (A-D ) Western blot and quantification of FASN, ACC1, SCD and ACLY in tumor tissues from a chemically induced murine CRC model ( A and B ) and CT26 allograft tumors ( C and D ). ( E ) Representative Oil Red O staining and BODIPY 493/503 staining of HCT-8 cells in the presence or absence of macrophage-conditioned medium are shown. Scale bars, 100 μm. ( F and G ) FASN expression in various colon cancer cells in the presence or absence of macrophage-conditioned medium was measured via western blotting. ( H ) Principal component analysis (PCA) model based on lipidomic analysis of the control and CM-treated cells. ( I ) Parametric volcano plot showing the fold change against the p value adjusted for the false discovery rate. Dark blue circles indicate downregulation (95 lipids), and dark red circles indicate upregulation (95 lipids) of lipids. ( J ) Number of various lipid molecules identified by lipidomics experiments. ( K ) Heatmap of differential lipids. ** P < 0.01, *** P < 0.001. Ctrl, control; CM, macrophage-conditioned medium
Article Snippet: The membranes were blocked in TBST containing 5% nonfat milk for 1 h at room temperature and incubated overnight with primary antibodies against FASN (Cat#10624-2-AP, Proteintech), USP14 (Cat#14517-1-AP, Proteintech), SREBP1 (Cat#14088-1-AP, Proteintech), ACC1 (Cat#21923-1-AP, Proteintech), SCD1 (Cat#28678-1-AP, Proteintech), ACLY (Cat#15421-1-AP, Proteintech) and β-actin (Cat#66009-1-Ig, Proteintech), followed by incubation with an HRP-labeled secondary antibody.
Techniques: Western Blot, Staining, Expressing, Control
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