h0802  (MedChemExpress)

Journal: Antioxidants

Article Title: Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism

doi: 10.3390/antiox15050525

Figure Lengend Snippet: H0802 improved hypoxia tolerance in mice. ( A ) Screening workflow. ( B ) Screening results of 44 compounds. ( C ) Survival curves of mice in a sealed hypoxia chamber ( n = 10). ( D ) Residual oxygen concentration in sealed chambers at the time of death ( n = 5). ( E ) Survival rates in the acute hypobaric hypoxia chamber experiment. ( n = 8). Data are presented as the mean ± SEM. *** p < 0.001, **** p < 0.0001 vs. control; ns, not significant. ( F , G ) Effects of H0802 on body weight ( F ) and food intake ( G ) in mice (100 mg/kg/day, i. g. once a day, 14-day, n = 5).

Article Snippet: H0802 (75 mg/kg) and acetazolamide (100 mg/kg, MCE, Cat. #HY-B0782) were administered by oral gavage daily for 3 consecutive days.

Techniques: Concentration Assay, Control

Journal: Antioxidants

Article Title: Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism

doi: 10.3390/antiox15050525

Figure Lengend Snippet: H0802- and hypoxia-treatment mice share common key changes in gene expression. ( A ) Workflow of RNA-seq analysis. ( B ) The heatmap of the shared gene between H0802- and hypoxia- treatment. ( C ) Volcano plots of differentially expressed genes (DEGs). ( D ) Correlation and functional enrichment analyses of DEGs. ( E ) GSEA for the 3 h H0802-treated group. ( F ) GO analysis of upregulated genes in the H0802-treated group. ( G ) GO analysis of downregulated genes in the H0802-treated group. ( H ) Validation results of the overlap between H0802-induced and hypoxia-induced DEGs. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. control; ns, not significant.

Article Snippet: H0802 (75 mg/kg) and acetazolamide (100 mg/kg, MCE, Cat. #HY-B0782) were administered by oral gavage daily for 3 consecutive days.

Techniques: Gene Expression, RNA Sequencing, Functional Assay, Biomarker Discovery, Control

Journal: Antioxidants

Article Title: Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism

doi: 10.3390/antiox15050525

Figure Lengend Snippet: H0802 promotes the stabilization and transcriptional activity of HIF-1α and HIF-2α. ( A ) H0802 enhances HIF-1α and HIF-2α proteins’ stability. ( B ) Quantification of HIF-1α and HIF-2α protein levels. ( C , D ) CHX chase assays in HepG2 cells. ( E ) The impacts of H0802 (40 μM) and roxadustat (10 μM) on endogenous HIF-1α protein ubiquitination. ( F ) QPCR analysis of HIF genes’ expression in HepG2 cells. Statistical significance: two-way ANOVA followed by Tukey’s post hoc test ( B , D , F ); * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. control. ns, not significant.

Article Snippet: H0802 (75 mg/kg) and acetazolamide (100 mg/kg, MCE, Cat. #HY-B0782) were administered by oral gavage daily for 3 consecutive days.

Techniques: Activity Assay, Ubiquitin Proteomics, Expressing, Control

Journal: Antioxidants

Article Title: Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism

doi: 10.3390/antiox15050525

Figure Lengend Snippet: H0802 regulates glucose metabolism and promotes glucose uptake. ( A ) Effects of hypoxia (10% O 2 ) or H0802 (100 mg/kg) on blood glucose levels in mice, n = 9. ( B ) Time-course changes in blood glucose levels in mice over 24 h following H0802 (100 mg/kg) treatment, n = 3. ( C ) Hepatic glycogen staining in mice treated with H0802 (100 mg/kg), scale bar: 100 μm, n = 3. ( D ) Effects of H0802 treatment on hepatic glycogen content in mice under normoxic or hypoxic conditions, n = 5. ( E ) Effects of H0802 treatment on mRNA expression of Slc2a1 in the brain tissue of mice under normoxic or hypoxic conditions, n = 6. ( F ) Glucose uptake in PC12 cells treated with DMSO or H0802 under normoxia or hypoxia. Data are shown as the mean ± SEM. p < 0.001. ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. control; ns, not significant.

Article Snippet: H0802 (75 mg/kg) and acetazolamide (100 mg/kg, MCE, Cat. #HY-B0782) were administered by oral gavage daily for 3 consecutive days.

Techniques: Staining, Expressing, Control

Journal: Antioxidants

Article Title: Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism

doi: 10.3390/antiox15050525

Figure Lengend Snippet: H0802 regulated oxygen consumption in mice. ( A ) Oxygen consumption in vehicle- or H0802-treated (75 mg/kg) mice. Values represent the mean ± SEM. ( B ) Carbon dioxide (CO 2 ) production in vehicle- or H0802-treated mice. ( C ) Respiratory quotient (RQ) in vehicle- or H0802-treated mice. ( D ) Energy expenditure (EE) in vehicle- or H0802-treated mice. Statistical significance was assessed using an unpaired two-sided Student’s t -test ( A – D ), n = 8. * p < 0.05 and ** p < 0.01 vs. control.

Article Snippet: H0802 (75 mg/kg) and acetazolamide (100 mg/kg, MCE, Cat. #HY-B0782) were administered by oral gavage daily for 3 consecutive days.

Techniques: Control

Journal: Antioxidants

Article Title: Thioamide Compound H0802 Enhances Hypoxia Tolerance by Mimicking Hypoxia-Adaptive Reprogramming of Glucose and Oxygen Metabolism

doi: 10.3390/antiox15050525

Figure Lengend Snippet: H0802 alleviates hypoxia-induced lung inflammation. ( A ) Representative hematoxylin–eosin (H&E) staining images. Scale bars: 5 mm (low-magnification overview) and 300 μm (high-magnification detail). ( B ) Quantification of lung injury scores. ( C ) Inhibitory effect of H0802 and acetazolamide on serum inflammatory cytokines (TNF-α, IL-6, IL-1β). ( D ) Differential gene expression analysis under hypoxia and H0802 treatment. ( E ) Validation results for Panel ( D ). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. control. ns, not significant.

Article Snippet: H0802 (75 mg/kg) and acetazolamide (100 mg/kg, MCE, Cat. #HY-B0782) were administered by oral gavage daily for 3 consecutive days.

Techniques: Staining, Gene Expression, Biomarker Discovery, Control

Journal: Applied and Environmental Microbiology

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae

doi: 10.1128/aem.02488-25

Figure Lengend Snippet: MoCA2, MoCA4, and MoCA6 subcellular localization, interaction with MoCA1, and function in Magnaporthe oryzae . ( A ) Subcellular localization in hyphae and conidia. Red fluorescence signals of MoCA2-RFP, MoCA4-RFP, and MoCA6-RFP protein of hyphae and conidia were examined by confocal microscopy and colocalized with Mito-Tracker Green on mitochondria. Scale bar = 10 μm. ( B ) Y2H between MoCA2, MoCA4, MoCA6, and MoCA1. MoCA2-AD, MoCA4-AD, MoCA6-AD, and MoCA1-BD were co-transformed into yeast strain Y2H Gold and cultured on SD−Leu−Trp and SD−Leu−Trp−His media. pGADT7-MoCA2/pGBKT7-MoCA1, pGADT7-MoCA4/pGBKT7-MoCA1, and pGADT7-MoCA6/pGBKT7-MoCA1, experimental group; pGADT7-T/pGBKT7-53 , positive control group; pGADT7/pGBKT7-MoCA1, negative control group; pGADT7-T/pGBKT7-lam , blank group. ( C ) BiFC assay demonstrating the interaction between MoCA2, MoCA4, MoCA6, and MoCA1. YFP fluorescence signals were observed in N. benthamiana leaves following co-expression of MoCA2-YC, MoCA4-YC, and MoCA6-YC (C-terminal fragment of YFP) with MoCA1-YN (N-terminal fragment of YFP). JC-1 staining confirmed mitochondrial membrane localization. Negative controls included MoCA2-YC, MoCA4-YC, MoCA6-YC, and YN alone. Scale bar = 5 μm.

Article Snippet: Wild-type and MoCA1 , MoCA2 , MoCA4 , MoCA6 knockout mutant strains were cultured in the PDA medium containing CA inhibitor-acetazolamide (Ace) (MCE, China) ( ) at a concentration of 50 nM in the experimental group and 0 nM in the control group at a temperature of 28°C, and the lesion area was counted on the 7th day.

Techniques: Fluorescence, Confocal Microscopy, Transformation Assay, Cell Culture, Positive Control, Negative Control, Bimolecular Fluorescence Complementation Assay, Expressing, Staining, Membrane

Journal: Applied and Environmental Microbiology

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae

doi: 10.1128/aem.02488-25

Figure Lengend Snippet: Analysis of synergistic effects of MoCA genes. ( A ) Colony morphology of WT, ∆ MoCA1 , and ∆ MoCA2 , ∆ MoCA4 , and ∆ MoCA6 on media with 0 nM and 50 nM carbonic anhydrase inhibitor. ( B ) Colony area measurement and statistical analysis. ( C ) Statistical analysis of MoCA gene expression in ACE of the WT strain. ( D ) Statistical analysis of MoCA1 gene expression in WT, Δ MoCA2 , Δ MoCA4 , and Δ MoCA6 . ( E ) Statistical analysis of MoCA2 gene expression in WT, Δ MoCA1 , Δ MoCA4 , and Δ MoCA6 . ( F ) Statistical analysis of MoCA4 gene expression in WT, Δ MoCA1 , Δ MoCA2 , and Δ MoCA6 . ( G ) Statistical analysis of MoCA6 gene expression in WT, Δ MoCA1 , Δ MoCA2 , and Δ MoCA4 . Error bars represent mean ± SD from three replicates. ns, P > 0.05; * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Wild-type and MoCA1 , MoCA2 , MoCA4 , MoCA6 knockout mutant strains were cultured in the PDA medium containing CA inhibitor-acetazolamide (Ace) (MCE, China) ( ) at a concentration of 50 nM in the experimental group and 0 nM in the control group at a temperature of 28°C, and the lesion area was counted on the 7th day.

Techniques: Gene Expression

Journal: Applied and Environmental Microbiology

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae

doi: 10.1128/aem.02488-25

Figure Lengend Snippet: Pathogenicity and invasive hyphal growth mechanisms of MoCA2 , MoCA4 , and MoCA6 . ( A ) Spray inoculation assay on rice leaves. Conidia suspensions of the WT, Δ MoCAs , and Δ MoCAs / CAs were used to infect rice leaves. ( B ) Lesion area analysis. ( C ) Statistical analysis of relative fungal growth. ( D ) Rice leaf sheath infection assay. The conidial suspension of indicated strains was injected into a rice sheath. Representative photographs of infectious hyphae were taken after 24, 48, and 72 h of incubation at 25°C. Scale bar = 10 μm. IH, infectious hyphae. ( E ) The infection rate was calculated according to the number of type 1 to type 4 events. The infection status of more than 100 germinated conidia per leaf sheath was scored at 24, 48, and 72 hpi. Type 1 has no penetration; type 2 only has a penetration peg or a single infectious hypha (IH); type 3 has more than two IH in one rice cell; type 4 has extensive IH into adjacent rice cells. Error bars represent at least three independently repeated standard deviations. Error bars represent at least three independently repeated standard deviations. ns, P > 0.05, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Wild-type and MoCA1 , MoCA2 , MoCA4 , MoCA6 knockout mutant strains were cultured in the PDA medium containing CA inhibitor-acetazolamide (Ace) (MCE, China) ( ) at a concentration of 50 nM in the experimental group and 0 nM in the control group at a temperature of 28°C, and the lesion area was counted on the 7th day.

Techniques: Infection, Suspension, Injection, Incubation

Journal: Applied and Environmental Microbiology

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae

doi: 10.1128/aem.02488-25

Figure Lengend Snippet: The role of MoCA2 , MoCA4, and MoCA6 in ATP synthesis. ( A ) Mitochondrial membrane potential test using the JC-1 dye. Confocal microscopy was used for observation, with CCCP as a positive control. Scale bar = 10 µm. (B）Ratio of red to green fluorescence intensity indicating mitochondrial membrane potential. ( C ) Statistical analysis of the ATP content of mutant strains. ( D ) Statistical analysis of ATP synthase-related gene expression in WT and knockout strains of M. oryzae . Error bars represent mean ± SD from three replicates. * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Wild-type and MoCA1 , MoCA2 , MoCA4 , MoCA6 knockout mutant strains were cultured in the PDA medium containing CA inhibitor-acetazolamide (Ace) (MCE, China) ( ) at a concentration of 50 nM in the experimental group and 0 nM in the control group at a temperature of 28°C, and the lesion area was counted on the 7th day.

Techniques: Membrane, Confocal Microscopy, Positive Control, Fluorescence, Mutagenesis, Gene Expression, Knock-Out

Journal: Applied and Environmental Microbiology

Article Title: Carbonic anhydrases contribute to mitochondrial function, conidial development, and pathogenicity of Magnaporthe oryzae

doi: 10.1128/aem.02488-25

Figure Lengend Snippet: The role of MoCA2, MoCA4, and MoCA6 in nitrogen metabolism. ( A ) Strains inoculated in MM (abundant-nitrogen medium), MM-N (nitrogen-free medium), MM-N (+Gln) (nitrogen-free medium with glutamic acid), and MM-N (+Glu) (nitrogen-free medium with glutamine). The hyphae were stained with Congo red dye and photographed for images. ( B ) Statistical analysis of colony size after 7 days of cultivation in abundant-nitrogen medium, nitrogen-free medium, nitrogen-free medium with glutamic acid, and nitrogen-free medium with glutamine. ( C ) Statistical analysis of gene expression in nitrogen metabolism of WT, Δ MoCA2 , Δ MoCA4 , and Δ MoCA6 . Error bars represent at least three independently repeated standard deviations. ns, P > 0.05; * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Wild-type and MoCA1 , MoCA2 , MoCA4 , MoCA6 knockout mutant strains were cultured in the PDA medium containing CA inhibitor-acetazolamide (Ace) (MCE, China) ( ) at a concentration of 50 nM in the experimental group and 0 nM in the control group at a temperature of 28°C, and the lesion area was counted on the 7th day.

Techniques: Staining, Gene Expression