ac-tub (Millipore)
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ac-tub
Ac Tub, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-tub/mouse+monoclonal+antibody+6c5/pmc11386216-63-21-25
Average 90 stars, based on 1 article reviews
Ac Tub, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ac-tub/mouse+monoclonal+antibody+6c5/pmc11386216-63-21-25
Average 90 stars, based on 1 article reviews
ac-tub - by Bioz Stars,
2026-09
90/100 stars
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1) Product Images from "Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease"
Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease
Journal: Stem Cells Translational Medicine
doi: 10.1093/stcltm/szae043
Figure Legend Snippet: Differential sensitivity to COPD- and non-COPD PBEC irradiation in ALI culture. (A) Schematic representation of the treatment plan and representative examples of immunofluorescent staining of 3D ALI culture at day 21 ALI from COPD for TP63, CK5, MUC5AC, and Ac-TUB. (B) Quantification of MUC5AC and (C) AcTUB, and (D) TP63 in the non-COPD and COPD cultures upon irradiation (0-2-4 Gy) at 21 days in ALI expressed as ratio of total cells. (E) Replating efficiency of non-COPD and COPD from ALI shows a reduced capacity for replating before and after 2, 4 Gy irradiation. N = 3 independent donors non-COPD and COPD per group. * P < .05; ** P < .01; *** P < .001.; **** P < .0001. Scale bar = 50 μm. Created with Biorender.
Techniques Used: Irradiation, Staining
Figure Legend Snippet: NOTCH inhibition reverts the pathological secretory phenotype and reduces radiation-induced DNA damage in COPD BSCs. A) Quantification of Ac-TUB+, MUC5A + cells in the ALI system shows that COPD cultures have a perturbed differentiation phenotype compared to non-COPD ALIs with an excess of MUC5A + cells at the expense of ciliated cells, which can be reverted with NOTCH inhibition. (B) Schematic representation of the treatment plan and quantification of the 53BP1 staining in TP63 + cells, 24 hours after RT in the presence or absence of NOTCH inhibition at day 19 to 21, 48 hours before RT (2-4 Gy). (C) Quantification of TP63, 24 hours after RT in the presence or absence of NOTCH inhibition at day 19 to 21, 48 hours prior to RT (2-4 Gy). N = 3 independent donors non-COPD and COPD per group * P < .05, ** P < .01; *** P < .001; **** P < .0001.
Techniques Used: Inhibition, Staining
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Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Irradiation:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Staining:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Inhibition:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Western Blot:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Immunostaining:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), CRISPR:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Amplification:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Reverse Transcription Polymerase Chain Reaction:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Two Tailed Test:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Marker:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Immunodetection:Article Title: Spontaneous Pancreatitis Caused by Tissue-Specific Gene Ablation of Hhex in Mice Article Snippet: Ac-Tub , Article Title: Developmental roles for Srf, cortical cytoskeleton and cell shape in epidermal spindle orientation Article Snippet: Primary antibodies used were: Srf (1:500, Santa-Cruz), MIIA (1:500, Covance), E-cadherin (1:200, ECCD-1, M. Takeichi), CD104 (1:200, 4-integrin, BD-Pharmigen), P-cadherin (1:100, Pcad, Zymed), Desmoplakin (1:300, Research Diagnostics), K14 (1:500, Fuchs Lab), K10 (1:500, Fuchs Lab), K6 (1:500, Fuchs Lab), Ki67 (1:500 Novocastra), cleaved Caspase 3 (1:200, Cell Signaling), β- and Article Title: Loss of NARS1 impairs progenitor proliferation in cortical brain organoids and leads to microcephaly Article Snippet: After washing with PBST 3 times for 5 min, sections were incubated with primary antibodies in 5% BSA in PBS at the following dilutions: SOX2 (Santa Cruz Biotechnology, sc17319, 1:100), TUJ1 (Biolegend, 801202, 1:1000), Cleaved Caspase 3 (Cell Signaling Technology, 9661S, 1:500), Ki67 (BD-Biosciences, 550609, 1:1000), PAX6 (BioLegend, PRB-278P, 1:300), phospho-Histone3 (Ser10) (Cell Signaling Technology, 9701, 1:500), CTIP2 (Abcam, ab28448, 1:500), TBR2 (Abcam, EPR19012, 1:250), pH3 (Abcam, ab80612, 1:500), αPKC (Abcam, ab4124, 1:250), Article Title: Amplification of olfactory signals by Anoctamin 9 is important for mammalian olfaction. Article Snippet: Sensing smells of foods, prey, or predators determines animal survival.. Olfactory sensory neurons in the olfactory epithelium (OE) detect odorants, where cAMP and Ca2+ play a significant role in transducing odorant inputs to electrical activity.. Here we show Anoctamin 9, a cation channel activated by cAMP/PKA pathway, is expressed in the OE and amplifies olfactory signals. Article Title: Mouse SAS-6 is required for centriole formation in embryos and integrity in embryonic stem cells Article Snippet: Ac-TUB , Article Title: Human airway organoids as 3D in vitro models for a toxicity assessment of emerging inhaled pollutants: Tire wear particles Article Snippet: In detail, the basement membrane matrix was depolymerized to harvest organoids from culture, and organoids were minimally washed with PBS and fixed in 4% paraformaldehyde for 10 min, blocked for 30 min, and incubated overnight with the primary antibodies p63 (1:200, ab124762, Abcam, United States), Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), |
