ac-tub  (Millipore)

Journal: Bioactive Materials

Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

doi: 10.1016/j.bioactmat.2026.01.026

Figure Lengend Snippet: Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.

Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

Techniques: Staining, Marker

Journal: Nature Communications

Article Title: Capturing disease severity in LIS1-lissencephaly reveals proteostasis dysregulation in patient-derived forebrain organoids

doi: 10.1038/s41467-025-64980-0

Figure Lengend Snippet: A Bar graphs of –log₁₀-transformed p -values from GO molecular function enrichment for actin binding, actin filament binding, tubulin binding, and cytoskeleton structural constituent; nominal significance p = 0.05; p -value adjusted with Bonferroni correction; two-sided Hypergeometric test. B Representative day 20 organoids stained for acetylated α-tubulin (Ac-TUB); yellow dashed lines, apical/basal VZ regions. C Quantification of Ac-TUB strand density at day 20: n = 11 control (4 cell lines), 10 mild, 12 moderate, 18 severe (2 cell lines each), across four differentiations. VZ loops: 41, 54, 49, 80, respectively; two-sided Wilcoxon test. D Day 20 organoids stained for N-cadherin (N-CAD); yellow dashed lines, VZ edges and N-cadherin diameter expansion. E Quantification of apical N-CAD signal at day 20: VZ structures n = 32 control, 39 mild, 42 moderate, 43 severe for a total of 6, 8, 7, and 9 differentiations, respectively; 2 cell lines each; two-sided Kruskal–Wallis with post hoc Wilcoxon correction. F GO enrichment for cadherin binding; nominal significance p = 0.05; p -value adjusted with Bonferroni correction; two-sided Hypergeometric test. G Heatmap of log₂FC for WNT pathway genes in cycling progenitors by severity grade versus control (* P < 0.05, ** P < 0.01, *** P < 0.001); two-sided Wilcoxon test. H WNT-GFP reporter organoids at day 20; yellow dashed lines, VZ boundaries. I Mean value of WNT-GFP intensity per VZ: n = 20 control (2 cell lines), 20 mild (2 cell lines), 10 moderate (one cell line), 10 severe (one cell line); 3 organoids per condition from two differentiations per cell line; two-sided Kruskal–Wallis with post hoc Wilcoxon correction. J Quantification of cell division plane orientation: n = 13 control, 12 mild, 12 moderate, 15 severe. VZ loops: 59, 56, 43, 57; cells: 206, 359, 172, 249, respectively; Chi-square test. K Examples of division planes in control and severe organoids; yellow dashed lines, VZ edges; white dashed lines, division plane orientation. Scale bars: B left 200 μm, B right, H 50 μm; D , K 20 μm; All boxplots show median, 25th/75th percentiles, and 1.5 interquartile range whiskers. Source data are provided as a Source Data file.

Article Snippet: Primary antibodies were diluted according to the manufacturer’s instructions and incubated overnight at 4 °C with the following dilutions: Ac-TUB (1:500, Cell Signaling, cat. no. 5335S), AFP (1:200, Sino Biologicals, cat.no.

Techniques: Transformation Assay, Binding Assay, Staining, Control

Journal: Stem Cells Translational Medicine

Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease

doi: 10.1093/stcltm/szae043

Figure Lengend Snippet: Differential sensitivity to COPD- and non-COPD PBEC irradiation in ALI culture. (A) Schematic representation of the treatment plan and representative examples of immunofluorescent staining of 3D ALI culture at day 21 ALI from COPD for TP63, CK5, MUC5AC, and Ac-TUB. (B) Quantification of MUC5AC and (C) AcTUB, and (D) TP63 in the non-COPD and COPD cultures upon irradiation (0-2-4 Gy) at 21 days in ALI expressed as ratio of total cells. (E) Replating efficiency of non-COPD and COPD from ALI shows a reduced capacity for replating before and after 2, 4 Gy irradiation. N = 3 independent donors non-COPD and COPD per group. * P < .05; ** P < .01; *** P < .001.; **** P < .0001. Scale bar = 50 μm. Created with Biorender.

Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Ac-TUB (1:1000; Cat #T7451-200UL; Sigma-Aldrich), TP63 (1:1000; Cat #ab124762; Abcam), CK5 (1:1000; Lot# B241498; Biolegend), and 53BP1 (1:750; Lot #612522; BD Biosciences).

Techniques: Irradiation, Staining

Journal: Stem Cells Translational Medicine

Article Title: Enhanced radiation sensitivity, decreased DNA damage repair, and differentiation defects in airway stem cells derived from patients with chronic obstructive pulmonary disease

doi: 10.1093/stcltm/szae043

Figure Lengend Snippet: NOTCH inhibition reverts the pathological secretory phenotype and reduces radiation-induced DNA damage in COPD BSCs. A) Quantification of Ac-TUB+, MUC5A + cells in the ALI system shows that COPD cultures have a perturbed differentiation phenotype compared to non-COPD ALIs with an excess of MUC5A + cells at the expense of ciliated cells, which can be reverted with NOTCH inhibition. (B) Schematic representation of the treatment plan and quantification of the 53BP1 staining in TP63 + cells, 24 hours after RT in the presence or absence of NOTCH inhibition at day 19 to 21, 48 hours before RT (2-4 Gy). (C) Quantification of TP63, 24 hours after RT in the presence or absence of NOTCH inhibition at day 19 to 21, 48 hours prior to RT (2-4 Gy). N = 3 independent donors non-COPD and COPD per group * P < .05, ** P < .01; *** P < .001; **** P < .0001.

Article Snippet: The samples were incubated overnight at 4 °C with the primary antibodies dissolved in blocking solution (MUC5 (1:1000; Cat #ab3649; Abcam), Ac-TUB (1:1000; Cat #T7451-200UL; Sigma-Aldrich), TP63 (1:1000; Cat #ab124762; Abcam), CK5 (1:1000; Lot# B241498; Biolegend), and 53BP1 (1:750; Lot #612522; BD Biosciences).

Techniques: Inhibition, Staining