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ab133534  (Proteintech)


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    Structured Review

    Proteintech ab133534
    Ab133534, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ab133534/pm41637189-528-133-143?v=Proteintech
    Average 94 stars, based on 4 article reviews
    ab133534 - by Bioz Stars, 2026-07
    94/100 stars

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    Image Search Results


    (A) Screen schematic: a Brunello KO Library of U2-OS cells (500x coverage) was treated with 5e3 vg/cell of the HB dual vectors. Cells were collected at 48 hpt, stained with MUG, and sorted by FACS. (B) Average fold-change of guide abundance (positive vs negative population) sorted and plotted as a waterfall plot. Rad51 and BRCA1 are highlighted. (C) Reactome pathway analysis results on the top 5% of genes in the positive population (candidate negative regulators of split transgene reconstitution), sorted by significance. Bubble size indicates the number of genes in each Reactome category.

    Journal: bioRxiv

    Article Title: Double-Strand Break Repair Pathways Differentially Affect Processing and Transduction by Dual AAV Vectors

    doi: 10.1101/2023.09.19.558438

    Figure Lengend Snippet: (A) Screen schematic: a Brunello KO Library of U2-OS cells (500x coverage) was treated with 5e3 vg/cell of the HB dual vectors. Cells were collected at 48 hpt, stained with MUG, and sorted by FACS. (B) Average fold-change of guide abundance (positive vs negative population) sorted and plotted as a waterfall plot. Rad51 and BRCA1 are highlighted. (C) Reactome pathway analysis results on the top 5% of genes in the positive population (candidate negative regulators of split transgene reconstitution), sorted by significance. Bubble size indicates the number of genes in each Reactome category.

    Article Snippet: 53BP1 – Abcam ab175188, 1:10,000; Phospho Histone H2A.X – Cell Signalling Technology 25775, 1:2500; BRCA1 Santa Cruz sc-6954 1:2000; Rad51 – Abcam ab133534, 1:1000.

    Techniques: Staining

    The effects of (A-C) Rad51 or (D-F) DNA-PKcs inhibition on split transgene reconstitution were tested in U2-OS cells by LAFA 48 hours post-transduction by the dual (or single) vector indicated above each plot. Bars represent the mean of 5 (A-C) or 4 (D-F) biological replicates; individual values are plotted as dots, bars represent SD. A 2-way ANOVA was used, with *p≤0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001. (G) U2-OSlacI-mNeonGreen cells were pretreated with DMSO or B02, transduced with 1e5 VG/cell AAV2/2.lacO.64, and immunostained for Rad51 (HDR) and 53BP1 (NHEJ) using a Cy5 secondary antibody at 48 hpt. VG and Cy5 foci overlap was quantified by high content imaging.

    Journal: bioRxiv

    Article Title: Double-Strand Break Repair Pathways Differentially Affect Processing and Transduction by Dual AAV Vectors

    doi: 10.1101/2023.09.19.558438

    Figure Lengend Snippet: The effects of (A-C) Rad51 or (D-F) DNA-PKcs inhibition on split transgene reconstitution were tested in U2-OS cells by LAFA 48 hours post-transduction by the dual (or single) vector indicated above each plot. Bars represent the mean of 5 (A-C) or 4 (D-F) biological replicates; individual values are plotted as dots, bars represent SD. A 2-way ANOVA was used, with *p≤0.05; **p≤0.01; ***p≤0.001; ****p≤0.0001. (G) U2-OSlacI-mNeonGreen cells were pretreated with DMSO or B02, transduced with 1e5 VG/cell AAV2/2.lacO.64, and immunostained for Rad51 (HDR) and 53BP1 (NHEJ) using a Cy5 secondary antibody at 48 hpt. VG and Cy5 foci overlap was quantified by high content imaging.

    Article Snippet: 53BP1 – Abcam ab175188, 1:10,000; Phospho Histone H2A.X – Cell Signalling Technology 25775, 1:2500; BRCA1 Santa Cruz sc-6954 1:2000; Rad51 – Abcam ab133534, 1:1000.

    Techniques: Inhibition, Transduction, Plasmid Preparation, Imaging

    (A) Early in transduction, rAAV vector genomes quickly associate with histones and are marked as double-strand breaks (DSB) by the host cell as evidenced by staining for ψH2AX. (B) HDR machinery appears to be recruited first (as detected with antibodies to BRCA1 and Rad51), which may block successful recruitment of NHEJ factors (such as DNA-PK and 53BP1). (C) Inhibition or loss of HDR machinery promotes increased expression and concatenation, which synergize to increase dual vector transduction.

    Journal: bioRxiv

    Article Title: Double-Strand Break Repair Pathways Differentially Affect Processing and Transduction by Dual AAV Vectors

    doi: 10.1101/2023.09.19.558438

    Figure Lengend Snippet: (A) Early in transduction, rAAV vector genomes quickly associate with histones and are marked as double-strand breaks (DSB) by the host cell as evidenced by staining for ψH2AX. (B) HDR machinery appears to be recruited first (as detected with antibodies to BRCA1 and Rad51), which may block successful recruitment of NHEJ factors (such as DNA-PK and 53BP1). (C) Inhibition or loss of HDR machinery promotes increased expression and concatenation, which synergize to increase dual vector transduction.

    Article Snippet: 53BP1 – Abcam ab175188, 1:10,000; Phospho Histone H2A.X – Cell Signalling Technology 25775, 1:2500; BRCA1 Santa Cruz sc-6954 1:2000; Rad51 – Abcam ab133534, 1:1000.

    Techniques: Transduction, Plasmid Preparation, Staining, Blocking Assay, Inhibition, Expressing