Journal: Acta Biochimica et Biophysica Sinica
Article Title: FGF8 promotes lipid droplet accumulation via the FGFR1/p-p38 axis in chondrocytes
doi: 10.3724/abbs.2025075
Figure Lengend Snippet: FGF8 induces lipid droplet accumulation in chondrocytes (A) KEGG analysis based on RNA sequencing data showing changes in signaling pathways in chondrocytes exposed to FGF8 at 25 ng/mL. The left side indicates upregulated pathways, and the right side indicates downregulated pathways. The red boxes refer to the signaling pathways closely associated with lipid droplet accumulation. (B) Representative fluorescence images showing changes in lipid droplets in chondrocytes exposed to different concentrations of FGF8. Chondrocytes were treated with FGF8 at 0, 25, 50, and 100 ng/mL for 2 days and then imaged using a 60× CLSM. Green fluorescence indicates lipids, red fluorescence indicates the cytoskeleton (F-actin), and blue fluorescence indicates the nuclei. The white dashed boxes indicate the magnified regions. (C) Total fluorescence quantification per cell confirming changes in lipid accumulation in chondrocytes treated with 0, 25, 50, or 100 ng/mL FGF8 for 2 days. The data were based on 15 cells from three independent samples (n = 3). (D) Quantification of the number of visible lipid droplets per cell, confirming the changes in the number of lipid droplets in chondrocytes exposed to 0, 25, 50, and 100 ng/mL FGF8 for two days. The data were based on 15 cells from three independent samples (n = 3). (E) Representative fluorescence images showing changes in lipid accumulation in cartilage caused by AAV-FGF8 overexpression. Green fluorescence indicates lipids, red fluorescence indicates Osx staining (negative in mature cartilage and positive in calcified cartilage), and blue fluorescence indicates the nuclei. The white dashed boxes indicate the magnified regions. (F) Quantification of the mean fluorescence intensity confirming the changes in lipid accumulation in the cartilage exposed to FGF8. The data were based on three replications (n = 3). The data in (C, D and F) were analyzed via one-way analysis of variance. The data in (C and D) were shown in box (from 25%, 50% to 75%) and whisker (minimum to maximum) plots. Differences were considered statistically significant at P < 0.05.
Article Snippet: AAV-FGF8 was obtained from GENECHEM (Shanghai, China).
Techniques: RNA Sequencing, Protein-Protein interactions, Fluorescence, Over Expression, Staining, Whisker Assay
