Journal: PLoS ONE
Article Title: Changes in Dietary Fat Content Rapidly Alters the Mouse Plasma Coagulation Profile without Affecting Relative Transcript Levels of Coagulation Factors
doi: 10.1371/journal.pone.0131859
Figure Lengend Snippet: Plasma clearance of FVIII (panel A) and the vitamin K-dependent coagulation factors VII (panel B) and IX (panel C) were determined in a separate experiment in which mice that were on a LFD (closed symbols) or HFD (open symbols) for 2 weeks received a single intravenous injection (200 μl) of either human FVIII concentrate (Aafact) or the human prothrombinase complex concentrate (Cofact). Clearance rates of the human plasma-derived factors from the mouse plasma were determined by successive blood sampling in EDTA vials via the tail vein and factor levels determination by ELISA. Values are the mean±SEM of six mice and expressed as the percentage of factor remaining in the circulation, with the amount of factor present at 1 minute after injection considered as 100%. Curves were calculated from the mean data using a one-exponential curve fit model.
Article Snippet: Plasma clearance of the vitamin K-dependent coagulation factors VIII, VII and IX were determined in a separate experiment in which mice fed a HFD for 2 weeks received a single intravenous injection (200 μl) of either the human FVIII concentrate (Aafact, Sanquin Plasma Products, Amsterdam, the Netherlands) or human prothrombinase complex concentrate (Cofact, Sanquin Plasma Products, Amsterdam, the Netherlands; both kindly provided by Dr. K. Mertens, Sanquin).
Techniques: Clinical Proteomics, Coagulation, Injection, Derivative Assay, Sampling, Enzyme-linked Immunosorbent Assay
