dgat1i a922500  (MedChemExpress)

Journal: iScience

Article Title: Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivation

doi: 10.1016/j.isci.2026.115274

Figure Lengend Snippet: The optimized culture condition increases lipid droplet formation (A) Gene function enrichment of upregulated genes in neoblasts cultured in the U-bottom plate with planarian extract compared with no extract. Fold change≥2, padj<0.05. (B) Particles are obviously increased inside SiRNeoblasts cultured in the U-bottom plate with planarian extract. The particles are pointed out by black arrows. Scale bars, 10 μm. (C) Lipid droplets stained with Nile red. More and larger lipid droplets exist in SiRNeoblasts under the optimized condition. Scale bars, 10 μm. (D) Flow cytometry analysis of Nile red signaling intensity in SiRNeoblasts. Blue histogram, culture in the U-bottom plate with the extract. Red histogram, culture in the U-bottom plate without the planarian extract. Orange histogram, culture in the plastic flat without the planarian extract. Gray histogram, SiRNeoblats without culture. (E) Flow cytometry analysis shows that 50 μM A922500 inhibits lipid droplet accumulation with treatment of planarian extract. (F) Inhibition of lipid droplet with 50 μM A922500 increases cell death after culture in the U-bottom plate with planarian extract for 3 days. Three biological replicates were assayed in each group. Data are represented as mean ± SEM. Unpaired two-tailed Student’s t test calculates p values. ∗∗∗0.0001 < p < 0.001. See also .

Article Snippet: For the lipid droplet inhibitor, 50 μM A922500 (TargetMol, T6365) was added to the culture medium when seeding cells before culture.

Techniques: Cell Culture, Staining, Flow Cytometry, Inhibition, Two Tailed Test

Journal: bioRxiv

Article Title: Synergistic activation of TLR2 and Dectin-2 by mannose-capped lipoarabinomannan reprograms macrophage lipid metabolism in tuberculosis

doi: 10.64898/2026.02.18.706227

Figure Lengend Snippet: A. iBMDM, control (C, non-targeting guide RNAs), TLR2 knock-down (KD), and Dectin-2 (Dec-2) KD iBMDM clones were left uninfected or infected with M. tuberculosis mc 2 6206 (MOI= 15) for 48h in triplicate wells, washed, and stained with Bodipy 493/503 and antibodies to the macrophage marker F4/80 for imaging flow cytometry. Data were generated and expressed as in . B. Representative microscopy images (60x magnification) of uninfected (U) and infected (Mtb) iBMDM in the experiment shown in panel A. Panel description as in . C, D. iBMDM were treated with ManLAM (500ng/ml) and vehicle-treated or treated with increasing doses of the diacylglycerol transferase inhibitor, A922500 (DGAT-i; 60, 90, 120ng/ml) and the acyl-coenzyme A:cholesterol acyltransferase inhibitor, CAS 615264-52-3 (ACAT-i; 5, 10, 15µg/ml). Imaging flow cytometry data for lipid droplet content were generated and expressed as in . *, p <0.05 by unpaired t test in all relevant panels. E. iBMDM were treated with ManLAM (500ng/ml) and vehicle-treated or treated with increasing doses of the PPARγ inhibitor GW9662 (0.5, 1, 2µM), mTORC1 inhibitor rapamycin (0.2, 0.4, 0.8nM), and the NF-κB inhibitor JSH-23 (3.5, 7, 14µM). Imaging flow cytometry data for lipid droplet content were generated and expressed as in . *, p <0.05 by unpaired t test in all relevant panels.

Article Snippet: Inhibitors of diacylglycerol transferase (A922500) and acyl-coenzyme A:cholesterol acyltransferase (CAS 615264-52-3), both from Santa Cruz Biotechnology, Dallas, TX, were stored as per manufacturer’s instructions.

Techniques: Control, Knockdown, Clone Assay, Infection, Staining, Marker, Imaging, Flow Cytometry, Generated, Microscopy