a600 phosphorylcholine macklin  (Quidel)

Journal: bioRxiv

Article Title: Neutrophils are critical for placental and fetal infection with the human pathogen Listeria monocytogenes

doi: 10.64898/2026.03.16.712023

Figure Lengend Snippet: (A) Percentage of blood cells positive for CFSE-labeled intracellular Lm obtained from peripheral blood of non-pregnant human adults exposed to CFSE-labeled Lm (mean±SEM, n=5 independent experiments, 1-way ANOVA, Tukey’s multiple comparison). (B) Percentage of blood cells positive for CFSE-labeled intracellular Lm from isolated non-pregnant human peripheral blood cells exposed to CFSE-labeled Lm (mean±SEM, n=4 independent experiments, 1-way ANOVA, Tukey’s multiple comparison). (C) CFUs of intracellular Lm of Lm-infected lysed human neutrophils, PBMCs and platelets (mean±SEM, n=3 independent experiments, 1-way ANOVA, Tukey’s multiple comparison). (D) Percentage of human non-pregnant neutrophils positive for intracellular CFSE-labeled Lm after blocking c-Met and/or FcγR (mean±SEM, n=5 independent experiments, 1-way ANOVA, Tukey’s multiple comparison). (E) Percentage of human non-pregnant neutrophils positive for intracellular CFSE-labeled Lm after serum treatment via heat inactivation (Hi) and Cobra venom factor (CVF) treatment. Neutrophils in Hank’s Balanced Salt Solution (HBSS) without Lm-infection served as control (mean±SEM, n=3-11 independent experiments, 1-way ANOVA, Tukey’s multiple comparison). *p <0.05, **p <0.01, ***p<0.001, ****p<0.0001.

Article Snippet: Prior to Listeria opsonization, human serum was de-complemented using 12 μg of cobra venom factor (CVF) (Quidel, San Diego, USA) per 500 μl of extracted serum and incubated for 1 h at 37 °C ( ).

Techniques: Labeling, Comparison, Isolation, Infection, Blocking Assay, Combined Bisulfite Restriction Analysis Assay, Control