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a4529  (Millipore)


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    Millipore a4529
    A4529, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a4529/aprotinin/pm38878288-144-72-74
    Average 90 stars, based on 1 article reviews
    a4529 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    Membrane:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    Real-time Polymerase Chain Reaction:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    Polymerase Chain Reaction:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    RNA Sequencing:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    Reporter Assay:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    Proliferation Assay:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.

    cDNA Synthesis:

    Article Title: m6A modification inhibits miRNAs' intracellular function, favoring their extracellular export for intercellular communication.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies m6A antibody Synaptic System Cat # 202 003; RRID:AB_2279214 Normal Rabbit IgG Millipore Cat # 12-370; RRID:AB_145841 Anti-METTL3 antibody Abcam Cat # 195352; RRID:AB_2721254 Anti-hnRNP A2B1 antibody Abcam Cat # Ab31645; RRID:AB_732978 Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Millipore Cat # MAB-374; RRID:AB_2107445 Anti Mouse Ago2 Wako Cat # 018-22021; RRID:AB_2096310 Goat Anti-Rabbit IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 172-1019; RRID:AB_11125143 Goat Anti-Mouse IgG (H + L)-HRP Conjugate Bio-Rad Laboratories Cat # 170-6516; RRID:AB_11125547 Normal Mouse IgG Millipore Cat # 12-371; RRID:AB_145840 Anti-CD9 Antibody Millipore Cat # CBL 162; RRID:AB_2075914 CD81 Antibody Santa-Cruz Biotechnology Cat # sc-166029; RRID:AB_2275892 Anti-LAMP1 antibody Abcam Cat # Ab25630; RRID:AB_470708 Calnexin Antibody Novus Biologicals Cat # NB100-1965; RRID:AB_10002123 Fatso (C-3) Santa Cruz Biotechnology Cat # sc-271713; RRID:AB_10707817 Chemicals, peptides, and recombinant proteins Dimethyl sulfoxide Sigma Cat #D2650-5X10MG Fetal Bovine Serum Gibco Cat # 10270 DMEM Gibco Cat # 21969-035 RPMI-1640 Gibco Cat # 31870-025 EGF Peprotech Cat # AF-100-15-1MG IGFII Peprotech Cat # 100-12-100UG Insulin Merck Cat #M376497001 Penicillin/Streptomycin Euroclone Cat # ECB3001D L-Glutamine Euroclone Cat # ECB3000D Collagen from rat tail Life Technologies Cat # A1048301 Bronchial tracheal epithelial growth medium Sigma-Aldrich Cat # 511/500 FB23-2 MedChemExpress Cat # HY-127103 STM2457 MedChemExpress Cat # HY-134836 Doxyciclin Sigma-Aldrich Cat #D9891 Tris Sigma Cat #T1503-5KG NaCl Carlo Erba Cat # 479687 NP-40 Fluka Cat # 74385 EDTA Carlo Erba Cat # 405463 SDS Fisher Bioreagents Cat # BP166-500 DynabeadsTM Protein G Invitrogen Cat # 100040 DynabeadsTM Protein A Invitrogen Cat # 100020 Bovine Serum Albumin Promega Cat #R396E Chloroform Carlo erba Cat # 438601 Qiazol Qiagen Cat # 79306 Glycogen Sigma-Aldrich Cat #G8751 Sodium Acetate Invitrogen Cat # AM9740 Ethanol Carlo Erba Cat # 414605 InterferaseTM -MazF enzyme Takara Cat # 2415A (Continued on next page) Cell Reports 43, 114369, June 25, 2024 15 .. REAGENT or RESOURCE SOURCE IDENTIFIER Lipofectamine LTX Reagent Thermo Fisher Scientific Cat # 15338100 FuGENE HD Transfection Reagent Promega Cat #E2311 DynabeadsTM M-280 Streptavidin Thermo Fisher Cat # 11205D KCl Carlo Erba Cat # 471177 MgCl2 Carlo Erba Cat # 459337 DTT Thermo Fisher Scientific Cat #R0861 EGTA Fluka Cat # 03778 Glycerol Carlo Erba Cat # 453752 RNAse inhibitor Promega Cat #N261B Leupeptin ThermoFisher Cat # 78435 Aprotinin Sigma Cat # A4529 PMSF Sigma Cat #P76-26 Sodium Orthovanadate Sigma Cat #S6508 Yeast tRNA Life Technologies Cat # 15401011 Laemli Alfa Aesar Cat #J61337 Triton X-100 Auros Organics Cat # 327372500 Acrylamide Bio-Rad Laboratories Cat # 1610148 APS Sigma Cat # A-7460 Temed Sigma Cat # 203-744-6 Nitrocellulose membrane Bio-Rad Laboratories Cat # 1620115 Nylon Membrane Amersham Cat # RPN.203N Glycine Fisher Bioreagents Cat # BP381-5 Methanol Merck Cat # 32213-2,5L-M Tween 20 Merck Cat # 8.22184.0500 Ponceau Sigma Cat #P7170 Nonfat-dried milk PanReac AppliChem Cat # A0830,0500 PageRulerTM Plus Prestained Protein Ladder Thermo Scientific Cat # 26619 ECL Bio-Rad Laboratories Cat # 1705062 Spike-In NORGEN Cat # 59000 GoTaq qPCR MasterMix Promega Cat # A600A Formamide Sigma Cat # F-9037 Formaldehyde AppliChem Cat # A0877,0250 Methylene blue Sigma Cat #M9140-25G PBS Euroclone Cat # ECB4004L DAPI ThermoFisher Scientific Cat # 62248 Critical commercial assays Look-out Mycoplasma PCR Detection Kit Merck Cat # MP0035 NEBNext Ultra II Directional RNA Library Prep kit New England Biolabs Cat #E7760L NEXTFLEX Small RNA Seq Kit v3 Bioo Scientific Cat # 5132-05 Dual-Luciferase Reporter Assay System Promega Cat #E1910 CellTiter 96 AQueous One Solution Cell Proliferation Assay Promega Cat #G3580 ReliaPrepTM RNA Miniprep Systems Promega Cat #Z6112 iScriptTM cDNA Synthesis Kit Bio-Rad Laboratories Cat # 1708891 miRNeasy Mini Kit Qiagen Cat # 1038703 RNeasy MinElute Cleanup Kit Qiagen Cat # 74204 mistiCq Sigma-Aldrich Cat # MIRRT-100RXN (Continued on next page) 16 Cell Reports 43, 114369, June 25, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data RNA and small RNA-sequencing This paper Zenodo (https://zenodo.org/deposit/7594655) Experimental models: Cell lines HepG2 ATCC N/A HepG2 shCTR this paper N/A HepG2 shMETTL3 this paper N/A Hep3B ATCC N/A Bw1J ATCC N/A D3 https://doi.org/10.1093/emboj/16.3.495 N/A HBEpC ATCC N/A Oligonucleotides Primers for miRNA expression are listed in Table S1 This paper N/A Primers for gene expression analyses are listed in Table S2 This paper N/A Recombinant DNA pLKOTet-On Lentiviral shRNA clones (shMETTL3) Dharmacon Cat # TRCN0000289812 pLKOTet-On Lentiviral shRNA clones (Non-Target shRNA Control) Dharmacon Cat # SHC202 TRC2 pmirGLO Dual-Luciferase miRNA Target Expression Vector Promega Cat #E1330 Software and algorithms ImageJ/FiJI NIH Schindelin et al.37 https://imagej.net/software/fiji/ Prism 9 GraphPad https://www.graphpad.com/ N/A ChemiDoc Imaging System Bio-Rad Cat# 12003153 Image Lab Software Bio-Rad Cat# 12012931 FastQC tool N/A v0.11.8 miRBase https://doi.org/10.1093/nar/gkq1027 Release 22. cutadapt https://doi.org/10.14806/ej.17.1.200 v4.1 Bowtie https://doi.org/10.1186/gb-2009-10-3-r25 v1.3.1 Samtools https://doi.org/10.1093/gigascience/giab008 v1.3.1 limma R package https://doi.org/10.1093/nar/gkv007 v3.54.0 miRGeneDB https://doi.org/10.1093/nar/gkab1101 2.1 UniversalMotif R package https://doi.org/10.18129/B9.bioc.universalmotif 1.20.0.



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