integrin α2β1 ligand peptide tfa tfa  (MedChemExpress)

Journal: Precision Clinical Medicine

Article Title: Blockade of integrin signaling reduces chemotherapy-induced premature senescence in collagen cultured bladder cancer cells

doi: 10.1093/pcmedi/pbac007

Figure Lengend Snippet: The integrin–AKT axis induced premature senescence via the p21/p53 pathway. ( A ) Schematic diagram of integrin reducing chemotherapy-induced cell apoptosis and promoting premature senescence through p53/p21 signaling. ( B ) Western blotting of ITGB1, phosphorylated AKT and total AKT in T24 cells (cultured in a dish or collagen gel) treated with PBS or MMC (0.5 μg/ml, 48 h). ( C ) Western blotting of p53 and p21 in T24 cells (cultured in a dish or collagen gel) treated with PBS or MMC (0.5 μg/ml, 48 h). ( D ) Western blotting of p53 and p21 in T24 cells (collagen culture, 0.5 μg/ml MMC treatment) pre-treated with PBS, Mir (10 nM), or TFA (1 μM). ( E ) SA-β-Gal-positive cells analysis of T24 cells (collagen culture, 0.5 μg/ml MMC treatment) pre-treated with PBS, Mir (10 nM), or TFA (1 μM). ( F ) Cell apoptosis of T24 cells (collagen culture, 0.5 μg/ml MMC treatment) pre-treated with PBS, Mir (10 nM) or TFA (1 μM). ( G ) Western blotting of ITGB1, phosphorylated AKT, total AKT, p53, and p21 in BP5 cells (cultured in a dish or collagen gel) treated with PBS or DOX (1 μg/ml, 48 h). ( H ) Western blotting of ITGB1, phosphorylated AKT, total AKT, p53, and p21 in BP7 cells (cultured in a dish or collagen gel) treated with PBS or DOX (1 μg/ml, 48 h).

Article Snippet: Integrin α2β1 ligand peptide TFA (TFA) and AKT inhibitor Miransertib (Mir) were purchased from MedChemExpress (USA).

Techniques: Western Blot, Cell Culture

Journal: Precision Clinical Medicine

Article Title: Blockade of integrin signaling reduces chemotherapy-induced premature senescence in collagen cultured bladder cancer cells

doi: 10.1093/pcmedi/pbac007

Figure Lengend Snippet: Blockade of integrin–ATK signaling suppressed chemotherapy-induced senescence in vivo . ( A ) Immunofluorescence of collagen in dish-cultured T24 or tumor tissues from T24-bearing mice. The scale bar is 50 μm. ( B ) Western blotting of ITGB1, phosphorylated AKT and total AKT in dish-cultured T24 or tumor tissues from T24-bearing mice. ( C ) Western blotting of p53 and p21 in tumor tissues from T24-bearing mice treated with PBS, MMC (5 mg/kg) or DOX (5 mg/kg) by tail vein injection. ( D ) SA-β-Gal-positive cells analysis in tumor tissues from T24-bearing mice treated with PBS, MMC (5 mg/kg), TFA (2.5 mg/kg) or combination. ( E ) Cell apoptosis in tumor tissues from T24-bearing mice treated with PBS, MMC (5 mg/kg), TFA (2.5 mg/kg) or combination. ( F ) Tumor volume of T24-bearing mice treated with PBS, MMC (5 mg/kg), TFA (2.5 mg/kg) or combination. ( G ) Overall survival of T24-bearing mice treated with PBS, MMC (5 mg/kg), TFA (2.5 mg/kg), or combination. n.s, no significant difference, *, P < 0.05, **, P < 0.01.

Article Snippet: Integrin α2β1 ligand peptide TFA (TFA) and AKT inhibitor Miransertib (Mir) were purchased from MedChemExpress (USA).

Techniques: In Vivo, Immunofluorescence, Cell Culture, Western Blot, Injection

Journal: Cancer Management and Research

Article Title: Extracellular Collagen Mediates Osteosarcoma Progression Through an Integrin α2β1/JAK/STAT3 Signaling Pathway

doi: 10.2147/CMAR.S273466

Figure Lengend Snippet: Type I collagen mediates tumor progression through integrin α2β1. ( A ) Western blotting of integrin α2 and integrin β1 in Saos-2 and Collagen-cultured Saos-2 cells. ( B ) Relative cell proliferation of collagen-cultured Saos-2 cells treated with α2β1 integrin ligand peptide (0.5mM, 48 hours). ( C ) tumor volume of collagen-cultured Saos-2 cells tumor-bearing mice pre-treated with α2β1 integrin ligand peptide (0.5 mM, 48 hours treatment before injection). ( D ) Relative colony formation of collagen pre-cultured Saos-2 cells pre-treated with α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours). ( E ) Tumorigenesis of collagen-cultured Saos-2 cells pre-treated with α2β1 integrin ligand peptide (0.5 mM, 48 hours treatment before injection) in NOS-SCID mice. ( F ) Relative transwell cells number of 2D collagen-cultured Sao-2 cells treated with PBS or α2β1 integrin ligand peptide (0.5mM, 48 hours). ( G ) the expression of integrin α2 and integrin β1 in the osteosarcoma tissues from HD and LD patients. ( H ) The correlation analysis of type I collagen expression and integrin α2 expression detected by Western blotting (R =0.5934). ( I ) The correlation analysis of type I collagen expression and integrin β1 expression detected by Western blotting (R =0.7431). *Indicates P <0.05.

Article Snippet: After 37°C incubation for 2 hours, the collagen mixtures became solid and cancer cells were seeded on the 2D collagen gel for 2D cells culture. α2β1 Integrin Ligand Peptide and STAT3 inhibitor FLLL32 were purchased from Med Chem Express (NJ, USA).

Techniques: Western Blot, Cell Culture, Injection, Expressing

Journal: Cancer Management and Research

Article Title: Extracellular Collagen Mediates Osteosarcoma Progression Through an Integrin α2β1/JAK/STAT3 Signaling Pathway

doi: 10.2147/CMAR.S273466

Figure Lengend Snippet: JAK/STAT3 acts as integrin downstream signal involved in tumor regulation. ( A ) the expression of phosphorylated and total JAK1, JAK2, STAT3 in Saos-2, collagen-cultured Saos-2 cells and collagen-cultured Saos-2 cells treated with α2β1 integrin ligand peptide (0.5mM, 48 hours). ( B ) Relative cell proliferation collagen-cultured Saos-2 cells treated with PBS or FLLL32 (1μM, 48 hours). ( C ) Tumor volume of Collagen-cultured Saos-2 cell tumor-bearing mice treated with PBS or FLLL32 (1 mg/kg, treated on day 14 and 17 after injection). ( D ) Relative colony formation of Collagen-cultured Saos-2 cells treated with PBS or FLLL32 (1μM, 48 hours). ( E ) Tumorigenesis of collagen treated Saos-2 cells treated with PBS or FLLL32 (1μM, 48 hours before injection) in NOD-SCID mice. ( F ) Expression of phosphorylated JAK1, JAK2 and STAT3 in tumor tissues from HD and LD patients. *Indicates P <0.05.

Article Snippet: After 37°C incubation for 2 hours, the collagen mixtures became solid and cancer cells were seeded on the 2D collagen gel for 2D cells culture. α2β1 Integrin Ligand Peptide and STAT3 inhibitor FLLL32 were purchased from Med Chem Express (NJ, USA).

Techniques: Expressing, Cell Culture, Injection

Journal: Cancer Management and Research

Article Title: Extracellular Collagen Mediates Osteosarcoma Progression Through an Integrin α2β1/JAK/STAT3 Signaling Pathway

doi: 10.2147/CMAR.S273466

Figure Lengend Snippet: Inhibition of integrin α2β1 enhances the therapeutic effects of chemotherapy/radiotherapy on osteosarcoma. ( A ) Cytotoxicity of PBS, IFO (2 mg/kg, 48 hours), and IFO (2 mg/kg, 48 hours) combined with α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) to Saos-2 and MG-63 cells. ( B ) Cytotoxicity of PBS, radiotherapy (0.5Gy) and radiotherapy (0.5Gy) combined with α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) to Saos-2 and MG-63 cells. ( C ) The presentative image and tumor volume of Saos-2-bearing mice treated with PBS, IFO (2 mg/kg, 48 hours), α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) and IFO combined with α2β1 integrin ligand peptide. The scale bar is 0.5 cm. ( D ) The survival time of Saos-2-bearing mice treated with PBS, IFO (2 mg/kg, 48 hours), α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours) and IFO combined with α2β1 integrin ligand peptide. ( E ) Tumor volumes of Saos-2-bearing mice treated with PBS, α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours), radiotherapy (0.5Gy), radiotherapy combined with α2β1 integrin ligand peptide. ( F ) the survival time of Saos-2-bearing mice treated with PBS, α2β1 integrin ligand peptide (0.5 mg/kg, 48 hours), radiotherapy (0.5Gy), radiotherapy combined with α2β1 integrin ligand peptide. *Indicates P <0.05, **Indicates P <0.01.

Article Snippet: After 37°C incubation for 2 hours, the collagen mixtures became solid and cancer cells were seeded on the 2D collagen gel for 2D cells culture. α2β1 Integrin Ligand Peptide and STAT3 inhibitor FLLL32 were purchased from Med Chem Express (NJ, USA).

Techniques: Inhibition