α ionone  (MedChemExpress)

Journal: Pharmacological research

Article Title: Microbiota-derived acetate suppresses sympathetic outflow via olfactory receptor 59 in the rostral ventrolateral medulla.

doi: 10.1016/j.phrs.2025.107766

Figure Lengend Snippet: Fig. 4. Inhibition of Olr59 in RVLM has no significant effect on ABP, A, Representative original records showed the effect of unilateral microinjection of α-ionone (1 mmol/L in 100 nL) into RVLM on ABP, HR, and RSNA in WKY rats. B, Bar charts show that unilateral microinjection of α-ionone into RVLM had no significant effect on MAP, HR, or RSNA in rats. n = 7, paired t-test. C. Schematic illustration of microinjection of AAV-Olr59 shRNA-EGFP in the RVLM of WKY rats to knockdown Olr59. D, Typical immunofluorescence staining pattern illustrates Olr59 knockdowns in the RVLM and presents a quantitative analysis of OLR59 fluorescence signal intensity. n = 4 per group, **P < 0.01, unpaired t-test. E, Knockdown of Olr59 in RVLM neurons had no effect on MAP or HR in WKY rats. n = 6 in each group, two-way ANOVA with Bonferroni’s post hoc test. WKY-EGFP, WKY rats injected with control virus; WKY-KD, Olr59 knockdown in RVLM of WKY rats.

Article Snippet: The changes in ABP, HR, and RSNA after microinjection of β-ionone (1 mmol/L, Cat# HY-W015084, MedChemExpress, USA), α-ionone (1 mmol/L, Cat# 03642, Merck, Germany), and sodium acetate (1 mmol/L, Cat# S2289, Sigma-Aldrich, USA) were recorded.

Techniques: Inhibition, Microinjection, shRNA, Knockdown, Immunofluorescence, Staining, Fluorescence, Injection, Control, Virus

Journal: Pharmacological research

Article Title: Microbiota-derived acetate suppresses sympathetic outflow via olfactory receptor 59 in the rostral ventrolateral medulla.

doi: 10.1016/j.phrs.2025.107766

Figure Lengend Snippet: Fig. 5. Acetate decreases blood pressure via Olr59 in RVLM, A, Representative traces show the effects of RVLM unilateral injection of α-ionone (1 mmol/L in 100 nL) and acetate (1 mmol/L in 100 nL) on ABP, HR, and RSNA in WKY rats. B, The bar charts show that after pretreatment with α-ionone, acetate did not decrease MAP or RSNA. n = 6, paired t-test. C, The representative original recordings show the effects of unilateral microinjection of acetate (1 mmol/L in 100 nL) into the RVLM on ABP, HR, and RSNA in Olr59 knockdown WKY rats. D, Mean data indicates no significant changes were found in MAP, HR, or RSNA in Olr59 knockdown rats following the microinjection of acetate into the RVLM. n = 6, paired t-test.

Article Snippet: The changes in ABP, HR, and RSNA after microinjection of β-ionone (1 mmol/L, Cat# HY-W015084, MedChemExpress, USA), α-ionone (1 mmol/L, Cat# 03642, Merck, Germany), and sodium acetate (1 mmol/L, Cat# S2289, Sigma-Aldrich, USA) were recorded.

Techniques: Injection, Microinjection, Knockdown