Journal: Botanical Studies
Article Title: Anti-ageing and attenuating cognitive declines of Dicliptera chinensis extracts and purified compounds in vitro and in scopolamine-induced cognitive dysfunction mice
doi: 10.1186/s40529-025-00478-8
Figure Lengend Snippet: A The flowchart of isolation protocol of compound 1 and compound 2 and the structures from the BuOH fractions of DC-95EE. The compound 3 was repeatedly re-crystallized from precipitates of BuOH fraction and aqueous layer of DC-95EE. B The HPLC fingerprints of DC-95EE. The HPLC chromatograms of compound 1 , compound 2 , and the overlapping figures of compound 1 and compound 2 . The arrows indicated the positions of compound 1 and compound 2 in the DC-95EE. The analytical Galaksil EF-C18 (5 μm, 250 × 4.6 mm) was performed using Shimadzu SCL-40 chromatography system with a gradient elution program was set in solvent mixtures of distilled water and acetonitrile as follows: water/acetonitrile, 90/10, at 0–35 min; 0/100, at 35–40 min; 90/10, at 40.01–55 min. The concentrations of DC-95EE, compound 1 , and compound 2 , respectively, were 100 mg/mL, 1 mg/mL, and 1 mg/mL, and 10 μL was injected for analysis. The flow rate was 1.0 mL/min and the wavelength was set at 335 nm for monitoring. The identified compounds included compound 1 (vicenin II, 7.415 min) and compound 2 (schaftoside, 8.112 min)
Article Snippet: The vicenin II and schaftoside with purity higher than 99.5% were purchased MedChemExpress LLC (NJ, USA).
Techniques: Isolation, Chromatography, Solvent, Injection