hy n0314  (MedChemExpress)

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: The construction and characterization of PF‐127@P dressings. (A) Image of PF‐127@P hydrogels at 4°C and 37°C. (B) Cumulative release rate (%) of PF‐127@P hydrogels containing different concentrations of pectolinarin at 1, 2, 3, 6, 12, 24 and 36 h in vitro.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: In Vitro

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Biocompatibility of PF‐127@P dressings. (A) Live/dead staining of coculture with dressing's extract for 1, 2 and 3 days, red is dead cells and green is live cells. (B) Hemolysis rate. * p < 0.05 when compared with PF‐127. (C) Cytoskeleton staining of coculture with dressing's extract for 1, 2 and 3 days, blue is nucleus and green is cytoskeleton. (D) Changes in body weight of experimental group (red, hydrogel wiping group) and blank group (black). (E) Major organ changes in rats with dressing (experimental) and control group.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Staining, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Dynamic process of wound healing under PF‐127@P dressings. (A) The dynamic process and schematic diagram of skin defect repair in rats with PF‐127@P hydrogels at Days 0, 3, 7, 10 and 14. (B–E) Wound healing rates at days 3, 7, 10 and 14. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Wound healing image and the scarless regeneration mechanism. (A) Haematoxylin and eosin and Masson staining images of wound on day 14 under PF‐127@P dressing. (B) The results of molecular docking experiments of the action of pectolinarin and YAP. (Yellow is the hydrogen bond, red is the site of pectolinarin binding on YAP and green is pectolinarin.) (C) Expression of α‐SMA; YAP and En1. α‐SMA, α‐smooth muscle Actin; En1, Engrailed‐1; YAP, Yes1 associated transcriptional regulator. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Staining, Binding Assay, Expressing, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Immunohistochemistry (IHC) image of wound healing. (A) Expression of CD34 (yellow arrows represented CD34) in the Control and PF‐127@50 groups on days 7 and 14. (B) Immunohistochemical statistics of vessel number in the Control and PF‐127@50 groups on days 7 and 14. (C) Statistical plot of YAP expression in tissues at day 14. (D) Statistical plot of YAP expression in tissues at day 14. (E) Expression of YAP in tissues at day 14. (F) Expression of α‐SMA in tissues at day 14. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Immunohistochemistry, Expressing, Control, Immunohistochemical staining

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: The construction and characterization of PF‐127@P dressings. (A) Image of PF‐127@P hydrogels at 4°C and 37°C. (B) Cumulative release rate (%) of PF‐127@P hydrogels containing different concentrations of pectolinarin at 1, 2, 3, 6, 12, 24 and 36 h in vitro.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: In Vitro

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Evaluation of the antioxidant capacity of pectolinarin. (A) Cell viability at different concentrations of pectolinarin (0, 5, 10, 20, 30 and 100 μg/mL). * p < 0.05 when compared with 0 μg/mL. (B) Cell viability under the effect of different concentrations of H 2 O 2 with (green) or without (white) 20 μg/mL of pectolinarin. (C) Fluorescence pictures of fibroblast ROS (green) under H 2 O 2 , Control, H 2 O 2 + Pec, cell nucleus (blue) and bright field cell pictures. (D) Comparison of fluorescence intensity under H 2 O 2 (white) and H 2 O 2 + Pec(green). Pec, pectolinarin; ROS, reactive oxygen species.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Fluorescence, Control, Comparison

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: The proliferation, migration and gene activation of endothelial cells. (A) Migration images at 12 h and 24 h. (B, E) Statistical analysis of scratch healing images at 12 h and 24 h. (C) Schematics of pectolinarin promoting angiogenesis. (D) Expression of angiogenic genes. (F) Proliferation of endothelial cells (Days 1, 2 and 3). ANG‐1, Angiopoietin 1; eNOS, endothelial nitric oxide synthase; VEGFR, vascular endothelial growth factor receptor; vWF, von Willebrand factor. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Migration, Activation Assay, Expressing, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Temperature‐sensitive hydrogel releasing pectolinarin facilitate scarless wound healing

doi: 10.1111/jcmm.18130

Figure Lengend Snippet: Wound healing image and the scarless regeneration mechanism. (A) Haematoxylin and eosin and Masson staining images of wound on day 14 under PF‐127@P dressing. (B) The results of molecular docking experiments of the action of pectolinarin and YAP. (Yellow is the hydrogen bond, red is the site of pectolinarin binding on YAP and green is pectolinarin.) (C) Expression of α‐SMA; YAP and En1. α‐SMA, α‐smooth muscle Actin; En1, Engrailed‐1; YAP, Yes1 associated transcriptional regulator. * p < 0.05 when compared with control.

Article Snippet: PF‐127 solutions containing pectolinarin (MedChemExpress, China) at concentrations of 0, 25, 50, 100 and 200 μg/mL were prepared in an ice bath, and the gel performance was evaluated.

Techniques: Staining, Binding Assay, Expressing, Control