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anti af647 secondary antibodies  (MedChemExpress)


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    MedChemExpress anti af647 secondary antibodies
    (A) RNA sequencing of the gene expression of neutrophils in the BALF of Casp1 -/- mice and Casp1 + / + mice at 12 hours after pulmonary E. coli infection. FPKM, fragments per kilobase per million mapped fragments. n = 2 for each group. (B) IP assay using anti-ZBP1 antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (C) IP assay using anti-dsRNA antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (D) Dot blot of MLE12 lysate sup, bac. Sup and pyrop. sup, as well as the control medium, via the dsRNA antibody. (E) Dot blot of BALF from the indicated chimeric mice at 12 hours after pulmonary E. coli infection using an anti-dsRNA antibody. (F) Neutrophil interactions of FITC-conjugated poly(I:C) and neutrophil death (PI + ) were measured by flow cytometry after stimulation with E. coli culture supernatant (bac. sup) or control medium with/without FITC-conjugated poly(I:C) (n = 3). (G) Representative immunofluorescence image of neutrophils cocultured with control/bac. sup and FITC-conjugated poly(I:C) using PKH26 and anti-ZBP1 antibodies and quantification of the percentage of <t>AF647/FITC</t> colocalized cells ( n = 3 biologically independent samples). Scale bars, 10 μm. (H) Neutrophils were pretreated with an anti-dsRNA antibody or a control IgG antibody and stimulated with pyrop. sup or control medium, and cell death was assayed by using flow cytometry (n = 3). (I) Survival of mice pretreated intratracheally with anti-dsRNA antibody (n = 7) or IgG control antibody (n = 6) 1 hour before pulmonary E. coli infection. This experiment was conducted across three independent replicates, with each replicate including 2–3 mice. (J and K) Neutrophil numbers (J) and proportions of PI + neutrophils (K) in the BALF of WT mice treated with anti-dsRNA antibody (n = 9) or IgG control antibody (n = 10) at 12 hours after pulmonary E. coli infection. The data are shown as the means ± SDs in (F, G, H, J and K) and are representative of 3 independent experiments in (B, C, D, E and G). Statistical differences were determined by the Mantel‒Cox test (I), two-way ANOVA (F and H) and Student’s t test (G, J and K). * P < 0.05; ** P < 0.01; **** P < 0.0001. ns, not significant. See also .
    Anti Af647 Secondary Antibodies, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti af647 secondary antibodies/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    anti af647 secondary antibodies - by Bioz Stars, 2026-02
    94/100 stars

    Images

    1) Product Images from "Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumonia"

    Article Title: Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumonia

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1013167

    (A) RNA sequencing of the gene expression of neutrophils in the BALF of Casp1 -/- mice and Casp1 + / + mice at 12 hours after pulmonary E. coli infection. FPKM, fragments per kilobase per million mapped fragments. n = 2 for each group. (B) IP assay using anti-ZBP1 antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (C) IP assay using anti-dsRNA antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (D) Dot blot of MLE12 lysate sup, bac. Sup and pyrop. sup, as well as the control medium, via the dsRNA antibody. (E) Dot blot of BALF from the indicated chimeric mice at 12 hours after pulmonary E. coli infection using an anti-dsRNA antibody. (F) Neutrophil interactions of FITC-conjugated poly(I:C) and neutrophil death (PI + ) were measured by flow cytometry after stimulation with E. coli culture supernatant (bac. sup) or control medium with/without FITC-conjugated poly(I:C) (n = 3). (G) Representative immunofluorescence image of neutrophils cocultured with control/bac. sup and FITC-conjugated poly(I:C) using PKH26 and anti-ZBP1 antibodies and quantification of the percentage of AF647/FITC colocalized cells ( n = 3 biologically independent samples). Scale bars, 10 μm. (H) Neutrophils were pretreated with an anti-dsRNA antibody or a control IgG antibody and stimulated with pyrop. sup or control medium, and cell death was assayed by using flow cytometry (n = 3). (I) Survival of mice pretreated intratracheally with anti-dsRNA antibody (n = 7) or IgG control antibody (n = 6) 1 hour before pulmonary E. coli infection. This experiment was conducted across three independent replicates, with each replicate including 2–3 mice. (J and K) Neutrophil numbers (J) and proportions of PI + neutrophils (K) in the BALF of WT mice treated with anti-dsRNA antibody (n = 9) or IgG control antibody (n = 10) at 12 hours after pulmonary E. coli infection. The data are shown as the means ± SDs in (F, G, H, J and K) and are representative of 3 independent experiments in (B, C, D, E and G). Statistical differences were determined by the Mantel‒Cox test (I), two-way ANOVA (F and H) and Student’s t test (G, J and K). * P < 0.05; ** P < 0.01; **** P < 0.0001. ns, not significant. See also .
    Figure Legend Snippet: (A) RNA sequencing of the gene expression of neutrophils in the BALF of Casp1 -/- mice and Casp1 + / + mice at 12 hours after pulmonary E. coli infection. FPKM, fragments per kilobase per million mapped fragments. n = 2 for each group. (B) IP assay using anti-ZBP1 antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (C) IP assay using anti-dsRNA antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (D) Dot blot of MLE12 lysate sup, bac. Sup and pyrop. sup, as well as the control medium, via the dsRNA antibody. (E) Dot blot of BALF from the indicated chimeric mice at 12 hours after pulmonary E. coli infection using an anti-dsRNA antibody. (F) Neutrophil interactions of FITC-conjugated poly(I:C) and neutrophil death (PI + ) were measured by flow cytometry after stimulation with E. coli culture supernatant (bac. sup) or control medium with/without FITC-conjugated poly(I:C) (n = 3). (G) Representative immunofluorescence image of neutrophils cocultured with control/bac. sup and FITC-conjugated poly(I:C) using PKH26 and anti-ZBP1 antibodies and quantification of the percentage of AF647/FITC colocalized cells ( n = 3 biologically independent samples). Scale bars, 10 μm. (H) Neutrophils were pretreated with an anti-dsRNA antibody or a control IgG antibody and stimulated with pyrop. sup or control medium, and cell death was assayed by using flow cytometry (n = 3). (I) Survival of mice pretreated intratracheally with anti-dsRNA antibody (n = 7) or IgG control antibody (n = 6) 1 hour before pulmonary E. coli infection. This experiment was conducted across three independent replicates, with each replicate including 2–3 mice. (J and K) Neutrophil numbers (J) and proportions of PI + neutrophils (K) in the BALF of WT mice treated with anti-dsRNA antibody (n = 9) or IgG control antibody (n = 10) at 12 hours after pulmonary E. coli infection. The data are shown as the means ± SDs in (F, G, H, J and K) and are representative of 3 independent experiments in (B, C, D, E and G). Statistical differences were determined by the Mantel‒Cox test (I), two-way ANOVA (F and H) and Student’s t test (G, J and K). * P < 0.05; ** P < 0.01; **** P < 0.0001. ns, not significant. See also .

    Techniques Used: RNA Sequencing, Gene Expression, Infection, Control, Dot Blot, Flow Cytometry, Immunofluorescence



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    anti af647 secondary antibodies  (MedChemExpress)
    94
    MedChemExpress anti af647 secondary antibodies
    (A) RNA sequencing of the gene expression of neutrophils in the BALF of Casp1 -/- mice and Casp1 + / + mice at 12 hours after pulmonary E. coli infection. FPKM, fragments per kilobase per million mapped fragments. n = 2 for each group. (B) IP assay using anti-ZBP1 antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (C) IP assay using anti-dsRNA antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (D) Dot blot of MLE12 lysate sup, bac. Sup and pyrop. sup, as well as the control medium, via the dsRNA antibody. (E) Dot blot of BALF from the indicated chimeric mice at 12 hours after pulmonary E. coli infection using an anti-dsRNA antibody. (F) Neutrophil interactions of FITC-conjugated poly(I:C) and neutrophil death (PI + ) were measured by flow cytometry after stimulation with E. coli culture supernatant (bac. sup) or control medium with/without FITC-conjugated poly(I:C) (n = 3). (G) Representative immunofluorescence image of neutrophils cocultured with control/bac. sup and FITC-conjugated poly(I:C) using PKH26 and anti-ZBP1 antibodies and quantification of the percentage of <t>AF647/FITC</t> colocalized cells ( n = 3 biologically independent samples). Scale bars, 10 μm. (H) Neutrophils were pretreated with an anti-dsRNA antibody or a control IgG antibody and stimulated with pyrop. sup or control medium, and cell death was assayed by using flow cytometry (n = 3). (I) Survival of mice pretreated intratracheally with anti-dsRNA antibody (n = 7) or IgG control antibody (n = 6) 1 hour before pulmonary E. coli infection. This experiment was conducted across three independent replicates, with each replicate including 2–3 mice. (J and K) Neutrophil numbers (J) and proportions of PI + neutrophils (K) in the BALF of WT mice treated with anti-dsRNA antibody (n = 9) or IgG control antibody (n = 10) at 12 hours after pulmonary E. coli infection. The data are shown as the means ± SDs in (F, G, H, J and K) and are representative of 3 independent experiments in (B, C, D, E and G). Statistical differences were determined by the Mantel‒Cox test (I), two-way ANOVA (F and H) and Student’s t test (G, J and K). * P < 0.05; ** P < 0.01; **** P < 0.0001. ns, not significant. See also .
    Anti Af647 Secondary Antibodies, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti af647 secondary antibodies/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    anti af647 secondary antibodies - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) RNA sequencing of the gene expression of neutrophils in the BALF of Casp1 -/- mice and Casp1 + / + mice at 12 hours after pulmonary E. coli infection. FPKM, fragments per kilobase per million mapped fragments. n = 2 for each group. (B) IP assay using anti-ZBP1 antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (C) IP assay using anti-dsRNA antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (D) Dot blot of MLE12 lysate sup, bac. Sup and pyrop. sup, as well as the control medium, via the dsRNA antibody. (E) Dot blot of BALF from the indicated chimeric mice at 12 hours after pulmonary E. coli infection using an anti-dsRNA antibody. (F) Neutrophil interactions of FITC-conjugated poly(I:C) and neutrophil death (PI + ) were measured by flow cytometry after stimulation with E. coli culture supernatant (bac. sup) or control medium with/without FITC-conjugated poly(I:C) (n = 3). (G) Representative immunofluorescence image of neutrophils cocultured with control/bac. sup and FITC-conjugated poly(I:C) using PKH26 and anti-ZBP1 antibodies and quantification of the percentage of AF647/FITC colocalized cells ( n = 3 biologically independent samples). Scale bars, 10 μm. (H) Neutrophils were pretreated with an anti-dsRNA antibody or a control IgG antibody and stimulated with pyrop. sup or control medium, and cell death was assayed by using flow cytometry (n = 3). (I) Survival of mice pretreated intratracheally with anti-dsRNA antibody (n = 7) or IgG control antibody (n = 6) 1 hour before pulmonary E. coli infection. This experiment was conducted across three independent replicates, with each replicate including 2–3 mice. (J and K) Neutrophil numbers (J) and proportions of PI + neutrophils (K) in the BALF of WT mice treated with anti-dsRNA antibody (n = 9) or IgG control antibody (n = 10) at 12 hours after pulmonary E. coli infection. The data are shown as the means ± SDs in (F, G, H, J and K) and are representative of 3 independent experiments in (B, C, D, E and G). Statistical differences were determined by the Mantel‒Cox test (I), two-way ANOVA (F and H) and Student’s t test (G, J and K). * P < 0.05; ** P < 0.01; **** P < 0.0001. ns, not significant. See also .

    Journal: PLOS Pathogens

    Article Title: Caspase-1-licensed pyroptosis drives dsRNA-mediated necroptosis and dampens host defense against bacterial pneumonia

    doi: 10.1371/journal.ppat.1013167

    Figure Lengend Snippet: (A) RNA sequencing of the gene expression of neutrophils in the BALF of Casp1 -/- mice and Casp1 + / + mice at 12 hours after pulmonary E. coli infection. FPKM, fragments per kilobase per million mapped fragments. n = 2 for each group. (B) IP assay using anti-ZBP1 antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (C) IP assay using anti-dsRNA antibody or IgG control antibody in BALF cells from WT mice at 12 hours after pulmonary E. coli infection. (D) Dot blot of MLE12 lysate sup, bac. Sup and pyrop. sup, as well as the control medium, via the dsRNA antibody. (E) Dot blot of BALF from the indicated chimeric mice at 12 hours after pulmonary E. coli infection using an anti-dsRNA antibody. (F) Neutrophil interactions of FITC-conjugated poly(I:C) and neutrophil death (PI + ) were measured by flow cytometry after stimulation with E. coli culture supernatant (bac. sup) or control medium with/without FITC-conjugated poly(I:C) (n = 3). (G) Representative immunofluorescence image of neutrophils cocultured with control/bac. sup and FITC-conjugated poly(I:C) using PKH26 and anti-ZBP1 antibodies and quantification of the percentage of AF647/FITC colocalized cells ( n = 3 biologically independent samples). Scale bars, 10 μm. (H) Neutrophils were pretreated with an anti-dsRNA antibody or a control IgG antibody and stimulated with pyrop. sup or control medium, and cell death was assayed by using flow cytometry (n = 3). (I) Survival of mice pretreated intratracheally with anti-dsRNA antibody (n = 7) or IgG control antibody (n = 6) 1 hour before pulmonary E. coli infection. This experiment was conducted across three independent replicates, with each replicate including 2–3 mice. (J and K) Neutrophil numbers (J) and proportions of PI + neutrophils (K) in the BALF of WT mice treated with anti-dsRNA antibody (n = 9) or IgG control antibody (n = 10) at 12 hours after pulmonary E. coli infection. The data are shown as the means ± SDs in (F, G, H, J and K) and are representative of 3 independent experiments in (B, C, D, E and G). Statistical differences were determined by the Mantel‒Cox test (I), two-way ANOVA (F and H) and Student’s t test (G, J and K). * P < 0.05; ** P < 0.01; **** P < 0.0001. ns, not significant. See also .

    Article Snippet: After drying, fixation, and permeabilization, the cells were stained with anti-ZBP1 and anti-AF647 secondary antibodies (MedChemExpress) for analysis.

    Techniques: RNA Sequencing, Gene Expression, Infection, Control, Dot Blot, Flow Cytometry, Immunofluorescence