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phenylephrine  (MedChemExpress)


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    MedChemExpress phenylephrine
    Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: <t>phenylephrine</t> (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.
    Phenylephrine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Norepinephrine stimulates protein synthesis in astrocytes"

    Article Title: Norepinephrine stimulates protein synthesis in astrocytes

    Journal: bioRxiv

    doi: 10.1101/2025.07.27.665649

    Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.
    Figure Legend Snippet: Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Techniques Used: Control, Western Blot



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    ( A ) A schematic of ICV injection of different noradrenergic receptor agonists and antagonists. ( B ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle. ( C–H ) Results show the effects of ICV injection of different doses of <t>phenylephrine,</t> prazosin, clonidine, yohimbine, isoprenaline, and propranolol on the recovery time of midazolam. ( I ) Results show the effects of ICV injection of phenylephrine (20 mg/mL) and different doses of propranolol on the recovery time of midazolam. ( J ) Protocol for PS of LC NE neurons and ICV injection or intra-VLPO microinjection of <t>α1-R</t> antagonist. ( K ) Schematic of the brief process of optogenetic activation. ( L ) The representative photomicrograph showing microinjection and optical fiber locations of the co-expression of hChR2 and TH in the LC. Moreover, quantification of the percentage of eYFP (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( M, O ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle and bilateral VLPO. ( N, P ) Results show the effects of PS of LC NE neurons and ICV injection or intra-VLPO microinjection of prazosin on the recovery time of midazolam. ( Q ) Protocol for chemogenetic activation of LC NE neurons and ICV injection or intra-VLPO microinjection of an α1-R antagonist. ( R ) Schematic of the location of virus injection. ( S ) The representative photomicrograph showing the co-expression of hM3Dq and TH in the LC of male mice. Moreover, quantification of the percentage of mCherry (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( T, V ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle and bilateral VLPO in the male mice. ( U, W ) Results show the effects of chemogenetic activation of LC NE neurons and ICV injection or intra-VLPO microinjection of prazosin on the recovery time of midazolam in male mice. ( X ) A representative photomicrograph showing the co-expression of hM3Dq and TH in the LC. Moreover, quantification of the percentage of mCherry (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( Y ) The tracks of cannulas implanted into the bilateral VLPO in the female mice. ( Z ) Results show the effects of chemogenetic activation of LC NE neurons and intra-VLPO microinjection of prazosin on the recovery time of midazolam in female mice. LC: locus coeruleus; VLPO: ventrolateral preoptic nucleus; i.p.: intraperitoneal injection; LORR: loss of righting reflex; RORR: recovery of righting reflex; TH: tyrosine hydroxylase; ICV: intracerebroventricular; PS: photostimulation; *p<0.05; **p<0.01; ***p<0.001.
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    Image Search Results


    Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Journal: bioRxiv

    Article Title: Norepinephrine stimulates protein synthesis in astrocytes

    doi: 10.1101/2025.07.27.665649

    Figure Lengend Snippet: Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Article Snippet: Phenylephrine (HY-B0471) and isoproterenol (HY-B0468) were from MedChemExpress.

    Techniques: Control, Western Blot

    Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Journal: bioRxiv

    Article Title: Norepinephrine stimulates protein synthesis in astrocytes

    doi: 10.1101/2025.07.27.665649

    Figure Lengend Snippet: Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Article Snippet: Phenylephrine (HY-B0471) and isoproterenol (HY-B0468) were from MedChemExpress.

    Techniques: Control, Western Blot

    Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Journal: bioRxiv

    Article Title: Norepinephrine stimulates protein synthesis in astrocytes

    doi: 10.1101/2025.07.27.665649

    Figure Lengend Snippet: Serum-deprived astrocytes were treated with 10 µM of the following adrenoreceptor (AR) agonists: phenylephrine (α 1 -AR), clonidine (α 2 -AR), isoproterenol (β-AR) or DMSO as a control for the indicated time points. 15 min immediately before sample collection, cells were labelled with 5 µM puromycin (PMY) and analysed by Western blot to detect phosphorylated S6 ribosomal protein kinase (pS6K, upper panel), S6 ribosomal protein (pS6, middle panel), and newly synthesised proteins (anti-PMY, lower panel). Quantification of signal intensities is presented here, normalised to untreated samples. Graphs show results from n=4 biological replicates (mean ± SEM). *p < 0.05, **p < 0.01, ***p < 0.001 (one-sample t-test comparing the means with a control mean of 1). Example blots are presented in Figure S2.

    Article Snippet: Phenylephrine (HY-B0471) and isoproterenol (HY-B0468) were from MedChemExpress.

    Techniques: Control, Western Blot

    ( A ) A schematic of ICV injection of different noradrenergic receptor agonists and antagonists. ( B ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle. ( C–H ) Results show the effects of ICV injection of different doses of phenylephrine, prazosin, clonidine, yohimbine, isoprenaline, and propranolol on the recovery time of midazolam. ( I ) Results show the effects of ICV injection of phenylephrine (20 mg/mL) and different doses of propranolol on the recovery time of midazolam. ( J ) Protocol for PS of LC NE neurons and ICV injection or intra-VLPO microinjection of α1-R antagonist. ( K ) Schematic of the brief process of optogenetic activation. ( L ) The representative photomicrograph showing microinjection and optical fiber locations of the co-expression of hChR2 and TH in the LC. Moreover, quantification of the percentage of eYFP (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( M, O ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle and bilateral VLPO. ( N, P ) Results show the effects of PS of LC NE neurons and ICV injection or intra-VLPO microinjection of prazosin on the recovery time of midazolam. ( Q ) Protocol for chemogenetic activation of LC NE neurons and ICV injection or intra-VLPO microinjection of an α1-R antagonist. ( R ) Schematic of the location of virus injection. ( S ) The representative photomicrograph showing the co-expression of hM3Dq and TH in the LC of male mice. Moreover, quantification of the percentage of mCherry (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( T, V ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle and bilateral VLPO in the male mice. ( U, W ) Results show the effects of chemogenetic activation of LC NE neurons and ICV injection or intra-VLPO microinjection of prazosin on the recovery time of midazolam in male mice. ( X ) A representative photomicrograph showing the co-expression of hM3Dq and TH in the LC. Moreover, quantification of the percentage of mCherry (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( Y ) The tracks of cannulas implanted into the bilateral VLPO in the female mice. ( Z ) Results show the effects of chemogenetic activation of LC NE neurons and intra-VLPO microinjection of prazosin on the recovery time of midazolam in female mice. LC: locus coeruleus; VLPO: ventrolateral preoptic nucleus; i.p.: intraperitoneal injection; LORR: loss of righting reflex; RORR: recovery of righting reflex; TH: tyrosine hydroxylase; ICV: intracerebroventricular; PS: photostimulation; *p<0.05; **p<0.01; ***p<0.001.

    Journal: eLife

    Article Title: NE contribution to rebooting unconsciousness caused by midazolam

    doi: 10.7554/eLife.97954

    Figure Lengend Snippet: ( A ) A schematic of ICV injection of different noradrenergic receptor agonists and antagonists. ( B ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle. ( C–H ) Results show the effects of ICV injection of different doses of phenylephrine, prazosin, clonidine, yohimbine, isoprenaline, and propranolol on the recovery time of midazolam. ( I ) Results show the effects of ICV injection of phenylephrine (20 mg/mL) and different doses of propranolol on the recovery time of midazolam. ( J ) Protocol for PS of LC NE neurons and ICV injection or intra-VLPO microinjection of α1-R antagonist. ( K ) Schematic of the brief process of optogenetic activation. ( L ) The representative photomicrograph showing microinjection and optical fiber locations of the co-expression of hChR2 and TH in the LC. Moreover, quantification of the percentage of eYFP (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( M, O ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle and bilateral VLPO. ( N, P ) Results show the effects of PS of LC NE neurons and ICV injection or intra-VLPO microinjection of prazosin on the recovery time of midazolam. ( Q ) Protocol for chemogenetic activation of LC NE neurons and ICV injection or intra-VLPO microinjection of an α1-R antagonist. ( R ) Schematic of the location of virus injection. ( S ) The representative photomicrograph showing the co-expression of hM3Dq and TH in the LC of male mice. Moreover, quantification of the percentage of mCherry (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( T, V ) A representative photomicrograph showing the tracks of cannulas implanted into the lateral ventricle and bilateral VLPO in the male mice. ( U, W ) Results show the effects of chemogenetic activation of LC NE neurons and ICV injection or intra-VLPO microinjection of prazosin on the recovery time of midazolam in male mice. ( X ) A representative photomicrograph showing the co-expression of hM3Dq and TH in the LC. Moreover, quantification of the percentage of mCherry (+)/TH (+) colocalization in all TH (+) neurons in LC is shown. ( Y ) The tracks of cannulas implanted into the bilateral VLPO in the female mice. ( Z ) Results show the effects of chemogenetic activation of LC NE neurons and intra-VLPO microinjection of prazosin on the recovery time of midazolam in female mice. LC: locus coeruleus; VLPO: ventrolateral preoptic nucleus; i.p.: intraperitoneal injection; LORR: loss of righting reflex; RORR: recovery of righting reflex; TH: tyrosine hydroxylase; ICV: intracerebroventricular; PS: photostimulation; *p<0.05; **p<0.01; ***p<0.001.

    Article Snippet: Three minutes before IP injection of midazolam, 2000 nL of the α1 receptor agonist phenylephrine (10 mg/mL, 20 mg/mL, n = 8, HY-B0471, MedChemExpress) or α1 receptor antagonist prazosin (0.75 mg/mL, 1.5 mg/ml, n = 8, HY-B0193, MedChemExpress) or α2 receptor agonist clonidine (0.75 mg/mL, 1.5 mg/mL, n = 9, HY-B0409, MedChemExpress) or α2 receptor yohimbine (15 μmol/mL, 22.5 μmol/mL, 30 μmol/mL, n = 8, Y111137, Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2.5 mg/mL, 5 mg/mL, n = 6, HY-B0573, MedChemExpress) or vehicle was administered via the lateral ventricle catheter.

    Techniques: Injection, Microinjection, Activation Assay, Expressing, Virus