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ranolazine dihydrochloride  (MedChemExpress)


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    MedChemExpress ranolazine dihydrochloride
    PITPNC1-mediated anoikis resistance is dependent on FAO metabolism. (A-E) BGC823 or AGS stably transfected with sh-PITPNC1 or ox-PITPNC1 were seeded and treated with 50 μM etomoxir for 48 h, and the metabolic state of the cells were detected as follows. (A) The basal respiration, maximal respiration, ATP production, and spare respiration capacity was measured after co-culture using Seahorse XF96 extracellular flux analyzer. (B) Fatty acid oxidation rate was measured by Fatty Acid β-Oxidation Kit. (C) ATP level was measured by firefly luciferase ATP Assay Kit. (D) ROS content was detected by measuring the fluorescence intensity of DCF-DA through flow cytometry. (E) Cell adhesion was detected by crystal violet staining after co-culture with adipocytes for 48 h. (F) Vector or PITPNC1 overexpressing BGC823 and AGS was seeded and treated with 50 μM etomoxir, and anoikis was detected by Calcein AM/EthD-1 staining method. (G) PITPNC1-silencing or overexpressing BGC823 was cultured in collagen I 3D culture systems and then treated with 50 μM etomoxir for 48 h. The sphere formation was photographed, and cleaved caspase 3 was examined as an indicator of anoikis by immunofluorescence. (H) The relative change in BAX, BCL2, SREBP1, PPARγ, CD36, CPT1B and PITPNC1 expression was analyzed by Western blot after PITPNC1 overexpression or silencing. Vector or PITPNC1 overexpressing BGC823 and AGS were seeded and treated with etomoxir (I) or <t>ranolazine</t> (J) , and the relative change in BAX, BCL2, CPT1B and PITPNC1 expression was analyzed by Western blot. (K) The nuclear-cytoplasmic distribution of PPARγ after PITPNC1 overexpression or silencing through immunofluorescence. (L) PITPNC1 overexpressing BGC823 and AGS were co-transfected with PPARγ silencing sequence, and the relative change in PPARγ, CPT1B and PITPNC1 expression was analyzed by Western blot. Error bars, SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.001.
    Ranolazine Dihydrochloride, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ranolazine dihydrochloride/product/MedChemExpress
    Average 90 stars, based on 5 article reviews
    ranolazine dihydrochloride - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming"

    Article Title: Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming

    Journal: Theranostics

    doi: 10.7150/thno.28219

    PITPNC1-mediated anoikis resistance is dependent on FAO metabolism. (A-E) BGC823 or AGS stably transfected with sh-PITPNC1 or ox-PITPNC1 were seeded and treated with 50 μM etomoxir for 48 h, and the metabolic state of the cells were detected as follows. (A) The basal respiration, maximal respiration, ATP production, and spare respiration capacity was measured after co-culture using Seahorse XF96 extracellular flux analyzer. (B) Fatty acid oxidation rate was measured by Fatty Acid β-Oxidation Kit. (C) ATP level was measured by firefly luciferase ATP Assay Kit. (D) ROS content was detected by measuring the fluorescence intensity of DCF-DA through flow cytometry. (E) Cell adhesion was detected by crystal violet staining after co-culture with adipocytes for 48 h. (F) Vector or PITPNC1 overexpressing BGC823 and AGS was seeded and treated with 50 μM etomoxir, and anoikis was detected by Calcein AM/EthD-1 staining method. (G) PITPNC1-silencing or overexpressing BGC823 was cultured in collagen I 3D culture systems and then treated with 50 μM etomoxir for 48 h. The sphere formation was photographed, and cleaved caspase 3 was examined as an indicator of anoikis by immunofluorescence. (H) The relative change in BAX, BCL2, SREBP1, PPARγ, CD36, CPT1B and PITPNC1 expression was analyzed by Western blot after PITPNC1 overexpression or silencing. Vector or PITPNC1 overexpressing BGC823 and AGS were seeded and treated with etomoxir (I) or ranolazine (J) , and the relative change in BAX, BCL2, CPT1B and PITPNC1 expression was analyzed by Western blot. (K) The nuclear-cytoplasmic distribution of PPARγ after PITPNC1 overexpression or silencing through immunofluorescence. (L) PITPNC1 overexpressing BGC823 and AGS were co-transfected with PPARγ silencing sequence, and the relative change in PPARγ, CPT1B and PITPNC1 expression was analyzed by Western blot. Error bars, SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.001.
    Figure Legend Snippet: PITPNC1-mediated anoikis resistance is dependent on FAO metabolism. (A-E) BGC823 or AGS stably transfected with sh-PITPNC1 or ox-PITPNC1 were seeded and treated with 50 μM etomoxir for 48 h, and the metabolic state of the cells were detected as follows. (A) The basal respiration, maximal respiration, ATP production, and spare respiration capacity was measured after co-culture using Seahorse XF96 extracellular flux analyzer. (B) Fatty acid oxidation rate was measured by Fatty Acid β-Oxidation Kit. (C) ATP level was measured by firefly luciferase ATP Assay Kit. (D) ROS content was detected by measuring the fluorescence intensity of DCF-DA through flow cytometry. (E) Cell adhesion was detected by crystal violet staining after co-culture with adipocytes for 48 h. (F) Vector or PITPNC1 overexpressing BGC823 and AGS was seeded and treated with 50 μM etomoxir, and anoikis was detected by Calcein AM/EthD-1 staining method. (G) PITPNC1-silencing or overexpressing BGC823 was cultured in collagen I 3D culture systems and then treated with 50 μM etomoxir for 48 h. The sphere formation was photographed, and cleaved caspase 3 was examined as an indicator of anoikis by immunofluorescence. (H) The relative change in BAX, BCL2, SREBP1, PPARγ, CD36, CPT1B and PITPNC1 expression was analyzed by Western blot after PITPNC1 overexpression or silencing. Vector or PITPNC1 overexpressing BGC823 and AGS were seeded and treated with etomoxir (I) or ranolazine (J) , and the relative change in BAX, BCL2, CPT1B and PITPNC1 expression was analyzed by Western blot. (K) The nuclear-cytoplasmic distribution of PPARγ after PITPNC1 overexpression or silencing through immunofluorescence. (L) PITPNC1 overexpressing BGC823 and AGS were co-transfected with PPARγ silencing sequence, and the relative change in PPARγ, CPT1B and PITPNC1 expression was analyzed by Western blot. Error bars, SD. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.001.

    Techniques Used: Stable Transfection, Transfection, Co-Culture Assay, Luciferase, ATP Assay, Fluorescence, Flow Cytometry, Staining, Plasmid Preparation, Cell Culture, Immunofluorescence, Expressing, Western Blot, Over Expression, Sequencing



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