nafamostat mesylate  (MedChemExpress)

Journal: bioRxiv

Article Title: Hepatocyte growth factor activator inhibitor-2 rapidly inactivates airway-expressed human Type II Transmembrane Serine Proteases

doi: 10.64898/2026.01.23.701355

Figure Lengend Snippet: A , Size-exclusion chromatography (SEC) chromatogram of the dasTMPRSS2:KD1 complex injected to a HiLoad 16/60 Superdex 75 124 mL gel filtration column. The two absorbance peaks are labelled as the TMPRSS2:KD1 complex and isolated KD1 protein. B , OThe 59 mL elution fraction was separated on an SDS-PAGE gel. The SDS-PAGE sample was prepared without reducing agent and was not thermally denatured in advance of gel separation. C , Overlaid SEC chromatograms of nafamostat-treated TMPRSS2 (pink trace) and TMPRSS2:KD1 (green trace). The chromatograms were plotted as a percentage relative to the first eluting peaks for each injection. D , SDS-PAGE gel separation of the TMPRSS2:nafamostat and TMPRSS2:KD1 complexes. Samples were either heated and reduced (+) or not heated and not reduced (-) prior to gel separation.

Article Snippet: Nafamostat mesylate (MedChemExpress Cat# HY-B0190A) and 6-amidino-2-naphthol methanesulfonate (TCI Cat# A1193) were prepared as fresh DMSO stocks immediately prior to inhibition assays.

Techniques: Size-exclusion Chromatography, Injection, Filtration, Isolation, SDS Page

Journal: Viruses

Article Title: ACE2-Decoy-Conjugated PLGA-PEG Nanoparticles Loaded with Nafamostat for Potent Antiviral Activity

doi: 10.3390/v17091167

Figure Lengend Snippet: Schematic diagram of nafamostat-loaded ACE2 decoy-modified nanoparticles.

Article Snippet: In brief, 30 μL of nafamostat solution (MedChemExpress, Zhenghe Bio-Technology Co., Ltd., Taiyuan, China) was emulsified in 1 mL of dichloromethane containing 10 mg/mL PLGA15k-PEG3.4k-COOH (Xi’an Ruixi Biological Technology Co. Ltd., Xi’an, China) by ultrasonic homogenization (80% amplitude, 1 min) to form the primary emulsion ( w / o ).

Techniques: Modification

Journal: Viruses

Article Title: ACE2-Decoy-Conjugated PLGA-PEG Nanoparticles Loaded with Nafamostat for Potent Antiviral Activity

doi: 10.3390/v17091167

Figure Lengend Snippet: Physical characterization of nafamostat-loaded nanoparticles (NM-PP NPs). ( A ) FT-IR spectra of NM, B-PP NPs, and NM-PP NPs. ( B – D ) Hydrodynamic diameter distribution ( B ), TEM ( C ), and zeta potential ( D ) of NM-PP NPs. ( E ) The particle size changes of NM-PP NPs investigated after placed at 4 °C and RT for 18 days ( n = 3). ( F ) In vitro release of NM-PP NPs (mean ± s.d., n = 3).

Article Snippet: In brief, 30 μL of nafamostat solution (MedChemExpress, Zhenghe Bio-Technology Co., Ltd., Taiyuan, China) was emulsified in 1 mL of dichloromethane containing 10 mg/mL PLGA15k-PEG3.4k-COOH (Xi’an Ruixi Biological Technology Co. Ltd., Xi’an, China) by ultrasonic homogenization (80% amplitude, 1 min) to form the primary emulsion ( w / o ).

Techniques: Zeta Potential Analyzer, In Vitro