candesartan cilexetil  (MedChemExpress)

Journal: Cell Death Discovery

Article Title: Single cell RNA sequencing reveals the role of local renin-angiotensin system in regulating ovarian physiological cycle and promoting PCOS

doi: 10.1038/s41420-025-02531-8

Figure Lengend Snippet: A The protocol of constructing and testing PCOS mouse model. B Representative microscopic images of the complete estrous cycle. C – E Representative estrous cyclicity of mice in the Control, PCOS, and Candesartan group during 8 consecutive days. F Proportion of each stage during 8 consecutive days. G – J Serum testosterone, anti-Müllerian hormone, luteinizing hormone and follicle stimulating hormone levels in mice. n = 8 per group, each point represents a sample, Control and Candesartan both vs. PCOS, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, “ns” for non-significant results.

Article Snippet: The in vivo experiments on female C57BL/6 mice aged 6 weeks were divided into three groups: 24 mice were randomly divided into (a) Control group with 8 mice; (b) PCOS group with 8 mice and (c) MSCs group with 8 mice. (a) mice were fed a chow diet (10% fat) and injected with DHEA solvent (soybean oil) into the back of the neck for 21 days; (b-c) mice were fed a high-fat diet (60% fat) and injected with DHEA (6 mg/kg/day, dissolved in DMSO and fused with soybean oil) into the back of the neck for 21 days; (c) mice were also treated with Candesartan cilexetil (3 mg/kg/day, dissolved in DMSO and fused with sterilized water, MedChemExpress, USA) via intragastric administration for 21 days.

Techniques: Control

Journal: Cell Death Discovery

Article Title: Single cell RNA sequencing reveals the role of local renin-angiotensin system in regulating ovarian physiological cycle and promoting PCOS

doi: 10.1038/s41420-025-02531-8

Figure Lengend Snippet: A – C The trajectory of body weight in mice, and statistical analysis of the area under the curve (AUC) and the area between the curves (ABC). D , G – H Serum fasting blood glucose and insulin levels in mice, and HOMA-IR, HOMA-IS and HOMA-β were calculated. E , F The trajectory of glucose tolerance test by intraperitoneal injection (ipGTT) and statistical analysis of the area under the curve AUC. I Serum total cholesterol, triglyceride and low density lipoprotein levels in mice. n = 8 per group, each point represents a sample, Control and Candesartan both vs. PCOS, ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, “ns” for non-significant results.

Article Snippet: The in vivo experiments on female C57BL/6 mice aged 6 weeks were divided into three groups: 24 mice were randomly divided into (a) Control group with 8 mice; (b) PCOS group with 8 mice and (c) MSCs group with 8 mice. (a) mice were fed a chow diet (10% fat) and injected with DHEA solvent (soybean oil) into the back of the neck for 21 days; (b-c) mice were fed a high-fat diet (60% fat) and injected with DHEA (6 mg/kg/day, dissolved in DMSO and fused with soybean oil) into the back of the neck for 21 days; (c) mice were also treated with Candesartan cilexetil (3 mg/kg/day, dissolved in DMSO and fused with sterilized water, MedChemExpress, USA) via intragastric administration for 21 days.

Techniques: Injection, Control

Journal: Cell Death Discovery

Article Title: Single cell RNA sequencing reveals the role of local renin-angiotensin system in regulating ovarian physiological cycle and promoting PCOS

doi: 10.1038/s41420-025-02531-8

Figure Lengend Snippet: A – C Representative images of the Control, PCOS, and Candesartan group ovarian tissue sections stained by H&E ( n = 6 per group). * show cystic follicles and # corpora lutea in microscopic images. D , E Single ovarian weight and the ratio of ovarian to body weight ( n = 16 per group). F , G The counting and proportion of cystic follicles and corpora lutea ( n = 6 per group). H The transcription and translation level of IRS1/PI3K/AKT mRNAs and proteins in mouse ovary ( n = 4 per group). I The transcription level of IRS1/PI3K/AKT mRNAs in mouse ovary ( n = 3 per group). J Representative images of western blot and the translation level of IRS1/PI3K/AKT (phosphorylated) proteins in mouse ovary ( n = 3 per group). Each point represents a sample, Control and Candesartan both vs. PCOS, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, “ns” for non-significant results.

Article Snippet: The in vivo experiments on female C57BL/6 mice aged 6 weeks were divided into three groups: 24 mice were randomly divided into (a) Control group with 8 mice; (b) PCOS group with 8 mice and (c) MSCs group with 8 mice. (a) mice were fed a chow diet (10% fat) and injected with DHEA solvent (soybean oil) into the back of the neck for 21 days; (b-c) mice were fed a high-fat diet (60% fat) and injected with DHEA (6 mg/kg/day, dissolved in DMSO and fused with soybean oil) into the back of the neck for 21 days; (c) mice were also treated with Candesartan cilexetil (3 mg/kg/day, dissolved in DMSO and fused with sterilized water, MedChemExpress, USA) via intragastric administration for 21 days.

Techniques: Control, Staining, Western Blot